Archives of Toxicology

, Volume 76, Issue 11, pp 664–670

Inhibitory effect of berberine on tert-butyl hydroperoxide-induced oxidative damage in rat liver

Authors

  • Jin-Ming Hwang
    • Department of Biochemistry, Medical College, Chung Shan Medical University, No. 110, Section 1, Chien Kuo N. Rd., Taichung 402, Taiwan
  • Chau-Jong Wang
    • Department of Biochemistry, Medical College, Chung Shan Medical University, No. 110, Section 1, Chien Kuo N. Rd., Taichung 402, Taiwan
  • Feu-Pi Chou
    • Department of Biochemistry, Medical College, Chung Shan Medical University, No. 110, Section 1, Chien Kuo N. Rd., Taichung 402, Taiwan
  • Tsui-Hwa Tseng
    • Department of Biochemistry, Medical College, Chung Shan Medical University, No. 110, Section 1, Chien Kuo N. Rd., Taichung 402, Taiwan
  • Yih-Shou Hsieh
    • Department of Biochemistry, Medical College, Chung Shan Medical University, No. 110, Section 1, Chien Kuo N. Rd., Taichung 402, Taiwan
  • Wea-Lung Lin
    • Department of Pathology, Medical College, Chung Shan Medical University, No. 110, Section 1, Chien Kuo N. Rd., Taichung 402, Taiwan
  • Chia-Yih Chu
    • Department of Biochemistry, Medical College, Chung Shan Medical University, No. 110, Section 1, Chien Kuo N. Rd., Taichung 402, Taiwan
Organ Toxicity and Mechanisms

DOI: 10.1007/s00204-002-0351-9

Cite this article as:
Hwang, J., Wang, C., Chou, F. et al. Arch Toxicol (2002) 76: 664. doi:10.1007/s00204-002-0351-9

Abstract.

Berberine, a main protoberberine component of Coptidis Rhizoma, was studied for the mechanism of its inhibitory effects on the tert-butyl hydroperoxide (t-BHP)-induced cytotoxicity and lipid peroxidation in rat liver. In the preliminary study, berberine expressed an antioxidant property by its capacity for quenching the free radicals of 1,1-diphenyl-2-picrylhydrazyl (DPPH). Further investigations were conducted using t-BHP-induced cytotoxicity in rat primary hepatocytes and hepatotoxicity in rats to evaluate the antioxidative bioactivity of berberine. The results in rat primary hepatocytes demonstrated that berberine, at the concentrations of 0.01–1.0 mM, significantly decreased the leakage of lactate dehydrogenase (LDH) and alanine aminotransferase (ALT), and the formation of malondialdehyde (MDA) induced by 30 min treatment of t-BHP (1.5 mM). Berberine also attenuated the t-BHP-induced depletion of glutathione (GSH) and induced a high level of DNA repair synthesis. The in vivo study showed that the intraperitoneal pretreatment with berberine (0.5 and 5 mg/kg) for 5 days before a single dose of t-BHP (0.1 mmol/kg) significantly lowered the serum levels of hepatic enzyme markers (ALT and aspartate aminotransferase) and reduced oxidative stress in the liver. The histopathological evaluation of the livers revealed that berberine reduced the incidence of liver lesions, including hepatocyte swelling, leukocyte infiltrations, and necrosis induced by t-BHP. These results lead us to speculate that berberine may play a chemopreventive role via reducing oxidative stress in living systems.

Berberine tert-Butyl hydroperoxide Cytotoxicity Genotoxicity Hepatocyte

Copyright information

© Springer-Verlag 2002