Skip to main content
Log in

Vegetation type and layer depth influence nitrite-dependent methane-oxidizing bacteria in constructed wetland

  • Short Communication
  • Published:
Archives of Microbiology Aims and scope Submit manuscript

Abstract

Nitrite-dependent anaerobic methane oxidation (n-damo) process might be an important methane sink in wetland system. However, information on n-damo microorganisms in constructed wetland (CW) system for water treatment is still lacking. The present study investigated the n-damo communities in five full-scale vertical-flow CW systems with different plants. N-damo bacterial abundance did not show a considerable shift in CW planted with Cyperus papyrus, but varied greatly in other CW systems. However, the evident vertical change of n-damo community diversity occurred in each CW system. These CW systems displayed the different vertical change trends for either n-damo community abundance or diversity. In addition, CW n-damo community structure could change with wetland layer depth. At a given wetland layer depth, the evident difference of n-damo community abundance, diversity and structure could be observed in the five different CW systems. Both wetland layer depth and vegetation type could contribute to the shift of n-damo bacterial abundance and community structure in CWs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

References

  • Bakhshoodeh R, Alavi N, Mohammadi AS, Ghanavati H (2016) Removing heavy metals from Isfahan composting leachate by horizontal subsurface flow constructed wetland. Environ Sci Pollut Res 23:12384–12391

    Article  CAS  Google Scholar 

  • Chen J, Zhou ZC, Gu JD (2015) Complex community of nitrite-dependent anaerobic methane oxidation bacteria in coastal sediments of the Mai Po wetland by PCR amplification of both 16S rRNA and pmoA genes. Appl Microbiol Biotechnol 99:1463–1473

    Article  CAS  PubMed  Google Scholar 

  • Chowdhury TR, Dick RP (2013) Ecology of aerobic methanotrophs in controlling methane fluxes from wetlands. Appl Soil Ecol 65:8–22

    Article  Google Scholar 

  • de la Varga D, Ruiz I, Alvarez JA, Soto M (2015) Methane and carbon dioxide emissions from constructed wetlands receiving anaerobically pretreated sewage. Sci Total Environ 538:824–833

    Article  PubMed  Google Scholar 

  • DeJournett TD, Arnold WA, LaPara TM (2007) The characterization and quantification of methanotrophic bacterial populations in constructed wetland sediments using PCR targeting 16S rRNA gene fragments. Appl Soil Ecol 35:648–659

    Article  Google Scholar 

  • Ettwig KF, van Alen T, van de Pas-Schoonen KT, Jetten MS, Strous M (2009) Enrichment and molecular detection of denitrifying methanotrophic bacteria of the NC10 phylum. Appl Environ Microbiol 75:3656–3662

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Fausser A, Dusek J, Cizkova H, Hoppert M, Walther P, Kazda M (2013) Internal oxygen dynamics in rhizomes of Phragmites australis and presence of methanotrophs in root biofilms in a constructed wetland for wastewater treatment. Desalin Water Treat 51:3026–3031

    Article  CAS  Google Scholar 

  • Guan W, Yin M, He T, Xie SG (2015) Influence of substrate type on microbial community structure in vertical-flow constructed wetland treating polluted river water. Environ Sci Pollut Res 22:16202–16209

    Article  CAS  Google Scholar 

  • Han P, Gu JD (2013) A newly designed degenerate PCR primer based on pmoA gene for detection of nitrite-dependent anaerobic methane-oxidizing bacteria from different ecological niches. Appl Microbiol Biotechnol 97:10155–10162

    Article  CAS  PubMed  Google Scholar 

  • He T, Guan W, Luan ZY, Xie SG (2016) Spatiotemporal variation of bacterial and archaeal communities in a pilot-scale constructed wetland for surface water treatment. Appl Microbiol Biotechnol 100:1479–1488

    Article  CAS  Google Scholar 

  • Hu BL, Shen LD, Lian X, Zhu Q, Liu S, Huang Q, He ZF, Geng S, Cheng DQ, Lou LP, Xu XY, Zheng P, He YF (2014) Evidence for nitrite-dependent anaerobic methane oxidation as a previously overlooked microbial methane sink in wetlands. Proc Natl Acad Sci U S A 111:4495–4500

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Jiang N, Wang Y, Dong X (2010) Methanol as the primary methanogenic and acetogenic precursor in the cold Zoige wetland at Tibetan Plateau. Microb Ecol 60:206–213

    Article  CAS  PubMed  Google Scholar 

  • Lee SH, Kang H (2016) The activity and community structure of total bacteria and denitrifying bacteria across soil depths and biological gradients in estuary ecosystem. Appl Microbiol Biotechnol 100:1999–2010

    Article  CAS  PubMed  Google Scholar 

  • Lee SH, Kim SY, Ding WX, Kang H (2015) Impact of elevated CO2 and N addition on bacteria, fungi, and archaea in a marsh ecosystem with various types of plants. Appl Microbiol Biotechnol 99:5295–5305

    Article  CAS  PubMed  Google Scholar 

  • Liu Y, Zhang JX, Zhao L, Li YZ, Yang YY, Xie SG (2015) Aerobic and nitrite-dependent methane-oxidizing microorganisms in sediments of freshwater lakes on the Yunnan Plateau. Appl Microbiol Biotechnol 99:2371–2381

    Article  CAS  PubMed  Google Scholar 

  • Luesken FA, Zhu BL, van Alen TA, Butler MK, Diaz MR, Song B, den Camp HJMO, Jetten MSM, Ettwig KF (2011) pmoA primers for detection of anaerobic methanotrophs. Appl Environ Microbiol 77:3877–3880

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Niu CZ, He ZX, Ge Y, Chang J, Lu ZM (2015) Effect of plant species richness on methane fluxes and associated microbial processes in wetland microcosms. Ecol Eng 84:250–259

    Article  Google Scholar 

  • Rietl AJ, Overlander ME, Nyman AJ, Jackson CR (2016) Microbial community composition and extracellular enzyme activities associated with Juncus roemerianus and Spartina alterniflora vegetated sediments in Louisiana saltmarshes. Microb Ecol 71:290–303

    Article  CAS  PubMed  Google Scholar 

  • Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, Hollister EB, Lesniewski RA, Oakley BB, Parks DH, Robinson CJ, Sahl JW, Stres B, Thallinger GG, Van Horn DJ, Weber CF (2009) Introducing MOTHUR: open-source, platform-independent, community supported software for describing and comparing microbial communities. Appl Environ Microbiol 75:7537–7541

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Shen LD, Liu S, He ZF, Lian X, Huang Q, He YF, Lou LP, Xu XY, Zheng P, Hu BL (2015a) Depth-specific distribution and importance of nitrite-dependent anaerobic ammonium and methane-oxidising bacteria in an urban wetland. Soil Biol Biochem 83:43–51

    Article  CAS  Google Scholar 

  • Shen LD, Huang Q, He ZF, Lian X, Liu S, He YF, Lou LP, Xu XY, Zheng P, Hu BL (2015b) Vertical distribution of nitrite-dependent anaerobic methane-oxidising bacteria in natural freshwater wetland soils. Appl Microbiol Biotechnol 99:349–357

    Article  CAS  PubMed  Google Scholar 

  • Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Tang YS, Wang L, Jia JW, Fu XH, Le YQ, Chen XZ, Sun Y (2011) Response of soil microbial community in Jiuduansha wetland to different successional stages and its implications for soil microbial respiration and carbon turnover. Soil Biol Biochem 43:638–646

    Article  CAS  Google Scholar 

  • Tian JQ, Chen H, Dong XZ, Wang YF (2012) Relationship between archaeal community structure and vegetation type in a fen on the Qinghai–Tibetan Plateau. Biol Fertil Soils 48:349–356

    Article  Google Scholar 

  • Tournebize J, Chaumont C, Fesneau C, Guenne A, Vincent B, Garnier J, Mander U (2015) Long-term nitrate removal in a buffering pondreservoir system receiving water from an agricultural drained catchment. Ecol Eng 80:32–45

    Article  Google Scholar 

  • Uggetti E, Hughes-Riley T, Morris RH, Newton MI, Trabi CL, Hawes P, Puigagut J, Garcia J (2016) Intermittent aeration to improve wastewater treatment efficiency in pilot-scale constructed wetland. Sci Total Environ 559:212–217

    Article  CAS  PubMed  Google Scholar 

  • Wang Y, Zhu G, Harhangi HR, Zhu B, Jetten MSM, Yin C, Op den Camp HJM (2012) Co-occurrence and distribution of nitrite-dependent anaerobic ammonium and methane-oxidizing bacteria in a paddy soil. FEMS Microbiol Lett 336:79–88

    Article  CAS  PubMed  Google Scholar 

  • Wang Y, Huang P, Ye F, Jiang Y, Song LY, Op den Camp HJM, Zhu GB, Wu SJ (2016) Nitrite-dependent anaerobic methane oxidizing bacteria along the water level fluctuation zone of the Three Gorges Reservoir. Appl Microbiol Biotechnol 100:1977–1986

    Article  CAS  PubMed  Google Scholar 

  • Yun JL, Zhang HX, Deng YC, Wang YF (2015) Aerobic methanotroph diversity in Sanjiang Wetland, Northeast China. Microb Ecol 69:567–576

    Article  PubMed  Google Scholar 

  • Zhang J, Wang Q, Fan JL, Xie HJ, Liu C, Liang S, Hu Z, Yang ZC, Zhao CC (2015) Comparisons of microbial abundance and community among different plant species in constructed wetlands in summer. Ecol Eng 82:376–380

    Article  Google Scholar 

  • Zhi W, Ji GD (2014) Quantitative response relationships between nitrogen transformation rates and nitrogen functional genes in a tidal flow constructed wetland under C/N ratio constraints. Water Res 64:32–41

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This work was supported by Integration Research and Demonstration of Water Quality Conservation and Ecological Restoration Technology of Urban Lake Landscape Water: the Major Projects of Synergy Creation of Production, Learning and Research of Guangzhou (No. 201508020078), and National Natural Science Foundation of China (Nos. 50908095, 41001323).

Author information

Authors and Affiliations

Authors

Corresponding authors

Correspondence to Shuguang Xie or Yan Long.

Ethics declarations

Conflict of interest

The authors declare that they have no competing interests.

Human and animal rights

This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Communicated by Erko Stackebrandt.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 13 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yang, M., Guo, Q., Tong, T. et al. Vegetation type and layer depth influence nitrite-dependent methane-oxidizing bacteria in constructed wetland. Arch Microbiol 199, 505–511 (2017). https://doi.org/10.1007/s00203-016-1328-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00203-016-1328-z

Keywords

Navigation