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Identification of “Bacillus cellulasensis” strain NIO-1130T as a member of Bacillus altitudinis and emendation of the latter

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Abstract

In the study by Mawlankar et al. in Arch Microbiol 198:83–89 (2016), the phylogenetic position of strain “Bacillus cellulasensis” NIO-1130T based on 16S rRNA and gyrB genes was inconsistent. Therefore, the aim of this study is to re-determine its taxonomic status using diverse genotypic approaches including single gene analysis, multilocus sequence analysis, and genomic analyses. The reconstructed phylogenetic trees based on 16S rRNA gene and six concatenated genes showed that “B. cellulasensis” NIO-1130T (=NCIM 5461T = CCTCC AB 2011126T) revealed the closest genetic relationship with type strain Bacillus altitudinis 41KF2bT, with 98.6–100 % similarities of 16S rRNA gene, gyrB, pycA, pyrE, mutL, aroE, trpB, and six concatenated housekeeping genes. The high similarities for gene(s) sequences between “B. cellulasensis” NIO-1130T and B. altitudinis 41KF2bT indicated that they should be conspecific. The DNA G+C content for strain NIO-1130T was determined to be 41.3 mol% and identical to that of B. altitudinis 41KF2bT. Moreover, 88.4 % of digital DNA–DNA hybridization and 98.7 % of average nucleotide identity values between two strains were much higher than the standard criteria for delineation of bacterial species, suggesting that they belonged to the same species. Therefore, the data from the combined genotypic analyses suggest that “Bacillus cellulasensis” should be classified as a member of Bacillus altitudinis.

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Fig. 1
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Abbreviations

MLSA:

Multilocus sequence analysis

dDDH:

Digital DNA–DNA hybridization

ANI:

Average nucleotide identity

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Acknowledgments

This work was financially supported by National Infrastructure of Natural Resources for Science and Technology Program of China (No. NIMR-2016-9) and COMRA program (No. DY125-15-R-01).

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Authors and Affiliations

  1. State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen, 361005, China

    Yang Liu, Qiliang Lai & Zongze Shao

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  1. Yang Liu
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  2. Qiliang Lai
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  3. Zongze Shao
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Correspondence to Zongze Shao.

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Communicated by Erko Stackebrandt.

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Liu, Y., Lai, Q. & Shao, Z. Identification of “Bacillus cellulasensis” strain NIO-1130T as a member of Bacillus altitudinis and emendation of the latter. Arch Microbiol 198, 835–838 (2016). https://doi.org/10.1007/s00203-016-1259-8

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  • DOI: https://doi.org/10.1007/s00203-016-1259-8

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