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Spongiimicrobium salis gen. nov., sp. nov., a bacterium of the family Flavobacteriaceae isolated from a marine sponge

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An Erratum to this article was published on 16 July 2016

Abstract

A Gram-stain-negative, strictly aerobic, pale-yellow pigmented, rod-shaped, chemoheterotrophic bacterium, designated A6F-11T, was isolated from a marine sponge collected in Japan. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the novel marine strain was affiliated with the family Flavobacteriaceae of the phylum Bacteroidetes and that it shared the highest (92.9 %) sequence similarity with Arenibacter palladensis LMG 21972T. The strain could be differentiated phenotypically from related members of the family Flavobacteriaceae. The major fatty acids of strain A6F-11T were iso-C15:1 G, iso-C15:0, C16:1 ω6c and/or C16:1 ω7c and iso-C17:0 3-OH. The polar lipid profile consisted of phosphatidylglycerol, two unidentified aminolipids and two unidentified lipids. The DNA G+C content was 34.7 mol%, and the major respiratory quinone was menaquinone 6 (MK-6). From the distinct phylogenetic position and combination of genotypic and phenotypic characteristics, the strain is considered to represent a novel taxon in the family Flavobacteriaceae, for which the name Spongiimicrobium salis gen. nov., sp. nov. is proposed. The type strain of S. salis gen. nov., sp. nov. is A6F-11T (= KCTC 42753T = NBRC 111401T).

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Acknowledgments

This work was supported by the New Energy and Industrial Technology Development Organization (NEDO), and in part by a research grant (2009–2011) of the Institute for Fermentation, Osaka, Japan.

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Authors and Affiliations

  1. College of Pharmacy, Keimyung University, 1095 Dalgubeoldaero, Dalseo-Gu, Daegu, 42601, Republic of Korea

    Jaewoo Yoon

  2. Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi, Iwate, 026-0001, Japan

    Kyoko Adachi

  3. Marine Biosciences Kamaishi Research Laboratory, Kitasato University, 160-4, Utou, Okirai, Sanriku-cho, Ofunato, Iwate, 022-0101, Japan

    Hiroaki Kasai

  4. Basic Research Laboratories, Nippon Steel & Sumikin Chemical Co., Ltd., 1-Tsukiji, Kisarazu, Chiba, 292-0835, Japan

    Kyoko Adachi

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  1. Jaewoo Yoon
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Correspondence to Jaewoo Yoon.

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Communicated by Erko Stackebrandt.

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203_2016_1227_MOESM1_ESM.pdf

Supplementary Fig. 1 Thin-layer chromatograms showing the total polar lipid compositions of A6F-11T. Total polar lipids were detected by spraying the plate with molybdophosphoric acid, molybdenum blue, α-naphthol and ninhydrin. PG: phosphatidylglycerol, UAL: unidentified amino lipid, UL: unidentified lipid (PDF 64 kb)

Supplementary Fig. 2 Negative results from the API 20E, API 50CH and API ZYM strips. (PDF 52 kb)

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Yoon, J., Adachi, K. & Kasai, H. Spongiimicrobium salis gen. nov., sp. nov., a bacterium of the family Flavobacteriaceae isolated from a marine sponge. Arch Microbiol 198, 663–670 (2016). https://doi.org/10.1007/s00203-016-1227-3

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