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Description of Hydrogenophaga laconesensis sp. nov. isolated from tube well water

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Abstract

A light cream color colony was isolated, using oligotrophic LB agar medium, from a water sample collected from a tube well and designated as HWB-10T. Cells of strain HWB-10T were Gram-negative, motile rods, non-spore forming, positive for catalase, oxidase, nitrate reduction and esculin. The predominant fatty acids were C16:0, summed feature 4 (C16:1 ω7c/iso-C15:0 2-OH) and summed feature 7 (C18:1 ω7c, C18:1 ω9t and/or C18:1 ω12t), and the major ubiquinone was Q-8. NCBI-BLAST- and EzTaxon-based 16S rRNA gene sequence similarity search identified strain HWB-10T as a member of the genus Hydrogenophaga and H. atypica DSM 15342T, H. defulvi DSM 15341T, H. palleronii LMG2366T and H. taeniospiralis LMG7170T being the nearest phylogenetic species with a similarity (%) of 99.3, 99.1, 98.4 and 98.2, respectively, while the similarity was <98.0 % with other species of the genus. However, DNA–DNA similarities between HWB-10T and H. atypica DSM 15342T and H. defulvi DSM 15341T were 37.0 and 43.0 %, respectively, indicating that strain HWB-10T is a novel species. Further, the DNA fingerprinting, based on BOX-, ERIC-, (GTG)5- and REP-PCR amplifications, distinguished strain HWB-10T from its closest species, H. atypica DSM 15342T and H. defulvi DSM 15341T with similarity coefficients of 0.45 and 0.37, respectively, a value sufficient to establish the species status within the genus Hydrogenophaga. In addition, strain HWB-10T exhibited several phenotypic differences with its closely related species. Based on the above cumulative differentiating characteristics, strain HWB-10T was identified as a new species of the genus Hydrogenophaga and proposed as Hydrogenophaga laconesensis sp. nov. with strain HWB-10T (KTCC 42478T = LMG 28681T) as its type strain.

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Acknowledgments

We acknowledge Council for Scientific and Industrial Research (CSIR), India, for funding INDIA’s IDEA (PSC0108) project. Our special thanks to Prof. Peter Kämpfer for kindly providing the types strains of species Hydrogenophaga defluvii (DSM 15341) and Hydrogenophaga atypica (DSM 15342). Our thanks to Dr. SWA Naqvi, Director, CSIR-NIO, for coordinating the activities of the INDIA’s IDEA project.

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  1. Laboratory for Conservation of Endangered Species (LaCONES), CSIR-Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad, 500007, India

    Soniya Mantri, Mohan Rao Chinthalagiri & Sathyanarayana Reddy Gundlapally

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  1. Soniya Mantri
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  2. Mohan Rao Chinthalagiri
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  3. Sathyanarayana Reddy Gundlapally
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Correspondence to Sathyanarayana Reddy Gundlapally.

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Communicated by Erko Stackebrandt.

GenBank/EMBL accession number for the 16S rRNA gene sequence of Hydrogenophaga laconesensis sp. nov. (HWB-10T) is KT756664.

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Mantri, S., Chinthalagiri, M.R. & Gundlapally, S.R. Description of Hydrogenophaga laconesensis sp. nov. isolated from tube well water. Arch Microbiol 198, 637–644 (2016). https://doi.org/10.1007/s00203-016-1224-6

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