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Massilia humi sp. nov. isolated from soil in Incheon, South Korea

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Abstract

A Gram-negative, strictly aerobic, non-motile, rod-shaped bacterium, designated THG-S6.8T, was isolated from soil in Incheon, South Korea. Based on 16S rRNA gene sequence, strain THG-S6.8T was moderately related to Massilia plicata 76T (97.3 %), Massilia dura 16T (97.2 %) and Massilia albidiflava 45T (96.9 %). Chemotaxonomic data revealed that strain THG-S6.8T possesses ubiquinone-8 as the predominant respiratory quinone, and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c) and C16:0 as the major fatty acids. The major polar lipids were found to be phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA–DNA relatedness between strain THG-S6.8T and Massilia plicata KCTC 12344T and Massilia dura KACC 12342T was 38.7 and 40.5 %, respectively. The DNA G+C content was 66.8 %. These data, together with phenotypic characterization, suggest the isolate represents a novel species, for which the name Massilia humi sp. nov. is proposed, with THG-S6.8T as the type strain (=KCTC 42737T = CCTCC AB 2015296T).

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Acknowledgments

This work was conducted under the industrial infrastructure program (No. N0000888) for fundamental technologies which is funded by the Ministry of Trade, Industry & Energy (MOTIE, Korea).

Author information

Authors and Affiliations

  1. Department of Oriental Medicinal Material & Processing College of Life Science, Kyung Hee University, 1 Seocheon, Kihung Yongin, Kyunggi, 446-701, Republic of Korea

    Juan Du

  2. Department of Acupuncture Meridian Science Research Center, College of Korean Medicine, Kyung Hee University, # 1 Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, Republic of Korea

    Chang Shik Yin

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  1. Juan Du
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Correspondence to Chang Shik Yin.

Additional information

Communicated by Erko Stackebrandt.

The NCBI GenBank accession number for the 16S rRNA gene sequence of strain THG-S6.8T is KM073948.

Electronic supplementary material

Below is the link to the electronic supplementary material.

The negative (–) properties of API ZYM, API 20NE and API 32GN tests of THG-S6.8T. (PPTX 50 kb)

203_2016_1195_MOESM2_ESM.pdf

Maximum-likelihood tree based on 16S rRNA gene sequence showing phylogenetic placement of strain THG-S6.8T and related members of the genus Massilia. Numbers at nodes represent bootstrap values over 50 % (1000 resampled datasets). (PDF 15 kb)

Transmission electron micrograph of cells of THG-S6.8T. Bar, 0.5 µm. (PDF 85 kb)

203_2016_1195_MOESM4_ESM.pdf

Two-dimensional TLC of total polar lipids of THG-S6.8T (a) and M. plicata KCTC 12344T (b). Abbreviations: phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) unidentified aminolipid (AL), unidentified lipids (L1-4) and unidentified phospholipid (PL) (PDF 53 kb)

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Du, J., Yin, C.S. Massilia humi sp. nov. isolated from soil in Incheon, South Korea. Arch Microbiol 198, 363–367 (2016). https://doi.org/10.1007/s00203-016-1195-7

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  • DOI: https://doi.org/10.1007/s00203-016-1195-7

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