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Haemophilus influenzae triggers autophagy in HEp-2 cells

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Abstract

The MAP-LC3 system regulates the intracellular formation of autophagy-associated vacuoles. These vacuoles contain the LC3 protein; thus it has been utilized as a marker to identify autophagosomes. The aim of our study was to investigate whether Haemophilus influenzae strains and their supernatants could activate autophagy in human larynx carcinoma cell line (HEp-2). We demonstrate that higher expression of the LC3B-II protein was induced, particularly by nontypeable Haemophilus influenzae (NTHi) 49766 and by supernatants, containing <50 kDa proteins, of both strains. Ultrastructural studies demonstrate vacuoles with a double membrane and/or membrane material inside, showing similar features to those of autophagic vacuoles. Together, our findings demonstrate that H. influenzae strains and their supernatants trigger an autophagic process.

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Acknowledgments

We thank V. Flores-López for help with the statistical data analysis, E. Rosales-Cruz for her assistance with the confocal microscopy, and J. Vidal-Graniel for critical reading of the manuscript. MREM has been a recipient of a scholarship from CONACyT, México to 2013. SGC and EOLV are the recipients of SNI, COFAA, and EDI fellowships.

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Authors and Affiliations

  1. Central de instrumentación de microscopía, Departamento de Investigación, Escuela Nacional de Ciencias Biológicas-Instituto Politécnico Nacional (ENCB- IPN), Prolongación de Carpio y Plan de Ayala, 11340, Mexico, DF, Mexico

    María del Rosario Espinoza-Mellado & Edgar O. López-Villegas

  2. Laboratorio de Bacteriología Médica, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas-Instituto Politécnico Nacional (ENCB- IPN), Prolongación de Carpio y Plan de Ayala, 11340, Mexico, DF, Mexico

    Carolina Reyes-Picaso, Miriam S. Garcés-Pérez, Cynthia V. Jardón-Serrano & Silvia Giono-Cerezo

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  1. María del Rosario Espinoza-Mellado
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  2. Carolina Reyes-Picaso
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  5. Edgar O. López-Villegas
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  6. Silvia Giono-Cerezo
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Correspondence to María del Rosario Espinoza-Mellado.

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Communicated by Erko Stackebrandt.

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Espinoza-Mellado, M.R., Reyes-Picaso, C., Garcés-Pérez, M.S. et al. Haemophilus influenzae triggers autophagy in HEp-2 cells. Arch Microbiol 198, 199–204 (2016). https://doi.org/10.1007/s00203-015-1167-3

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  • DOI: https://doi.org/10.1007/s00203-015-1167-3

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