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Brevibacterium metallicus sp. nov., an endophytic bacterium isolated from roots of Prosopis laegivata grown at the edge of a mine tailing in Mexico

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Abstract

A Gram-positive, aerobic, nonmotile strain, NM2E3T was identified as Brevibacterium based on the 16S rRNA gene sequence analysis and had the highest similarities to Brevibacterium jeotgali SJ5-8T (97.3 %). This novel bacterium was isolated from root tissue of Prosopis laegivata grown at the edge of a mine tailing in San Luis Potosí, Mexico. Its cells were non-spore-forming rods, showing catalase and oxidase activities and were able to grow in LB medium added with 40 mM Cu2+, 72 mM As5+ and various other toxic elements. Anteiso-C15:0 (41.6 %), anteiso-C17:0 (30 %) and iso-C15:0 (9.5 %) were the major fatty acids. MK-8(H2) (88.4 %) and MK-7(H2) (11.6 %) were the major menaquinones. The DNA G + C content of the strain NM2E3T was 70.8 mol % (Tm). DNA–DNA hybridization showed that the strain NM2E3T had 39.8, 21.7 and 20.3 % relatedness with B. yomogidense JCM 17779T, B. jeotgali JCM 18571T and B. salitolerans TRM 45T, respectively. Based on the phenotypic and genotypic analyses, the strain NM2E3T (=CCBAU 101093T = HAMBI 3627T = LMG 8673T) is reported as a novel species of the genus Brevibacterium, for which the name Brevibacterium metallicus sp. nov., is proposed.

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Acknowledgments

This research was funded with Projects SIP-IPN 20130722 and 20130828. B.R.P. received scholarships support from the CONACyT and BEIFI. M.S.V.M., P.E.dl.S. and E.T.W. appreciate the scholarships funded by COFAA and EDI-IPN and SNI-CONACyT. This research was partially supported by China Natural Foundation (No. 31270052) to W. F. C.

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Authors and Affiliations

  1. Departamento de Microbiología, Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, México, D. F., 11340, Mexico

    Brenda Román-Ponce, María Soledad Vásquez-Murrieta, Wen Feng Chen, Paulina Estrada-de los Santos & En Tao Wang

  2. State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China

    Yong Hua Li, Xin Hua Sui & En Tao Wang

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  1. Brenda Román-Ponce
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Correspondence to En Tao Wang.

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Additional information

Communicated by Erko Stackebrandt.

The accession number of the 16S rRNA gene for the type strain NM2E3T in GenBank is KM874400.

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Román-Ponce, B., Li, Y.H., Vásquez-Murrieta, M.S. et al. Brevibacterium metallicus sp. nov., an endophytic bacterium isolated from roots of Prosopis laegivata grown at the edge of a mine tailing in Mexico. Arch Microbiol 197, 1151–1158 (2015). https://doi.org/10.1007/s00203-015-1156-6

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