Abstract
The present investigation deals with the optimization of cell growth rate of the candidate probiotic Pediococcus acidilactici in the presence of the specific prebiotic inulin. Three independent variables viz. concentration of inulin, concentration of glucose and pH have been selected for optimization study using response surface methodology. Theoretical analysis indicates that the maximum cell growth rate occurs at pH 7, 20 g/dm3 concentration of inulin and 20 g/dm3 concentration of glucose. Validation of these values has been done through a set of programmed experiments. Studies on cell dynamics in the presence of different concentrations of inulin have also been carried out to identify any limitation on the initial inulin concentration. Results clearly indicate that cell growth is enhanced with the increase in inulin concentration. However, there is a critical value of the prebiotic concentration (20 g/dm3 inulin) beyond which the cell growth is inhibited. A summative type growth model has been proposed to explain the growth behaviour of P. acidilactici in the presence of the dual substrate, i.e. glucose and inulin. While growth on glucose follows Monod model, Haldane-type substrate-inhibited growth model holds good for growth on inulin. Intrinsic kinetic parameters for all the model equations have been determined experimentally.
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The authors are thankful to University Grant Commission for providing UGC-BSR fellowship to the first author.
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Communicated by Erko Stackebrandt.
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Samanta Koruri, S., Chowdhury, R. & Bhattacharya, P. Analysis of cell growth dynamics of Pediococcus acidilactici in the presence of inulin in an optimized microenvironment. Arch Microbiol 197, 955–963 (2015). https://doi.org/10.1007/s00203-015-1131-2
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DOI: https://doi.org/10.1007/s00203-015-1131-2