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Sphingomonas flavus sp. nov. isolated from road soil

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Abstract

A yellow-colored, Gram-negative, strictly aerobic, non-motile, rod-shaped bacterium, designated THG-MM5T, was isolated from road soil in Yongin-si, Gyeonggi-do, Republic of Korea. Based on 16S rRNA gene sequence, strain THG-MM5T was moderately related to Sphingomonas sediminicola KACC 15039T (96.1 %), Sphingomonas ginsengisoli KACC 16858T (96.1 %) and Sphingomonas jaspsi KACC 13230T (96.0 %). Chemotaxonomic data revealed that strain THG-MM5T possesses ubiquinone-10 as the only respiratory quinone, sym-homospermidine as the major polyamine and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), C18:1 ω7c and C16:0 as the major fatty acids. The polar lipid profile included sphingoglycolipid. The DNA G + C content was 60.7 mol%. These data, together with phenotypic characterization, corroborated the affiliation of strain THG-MM5T to the genus Sphingomonas. Thus, the isolate represents a novel species, for which the name Sphingomonas flavus sp. nov. is proposed, with THG-MM5T as the type strain (=KACC 18277T = CCTCC AB 2014320T).

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Acknowledgments

This work was conducted under the industrial infrastructure program (No. N0000888) for fundamental technologies which is funded by the Ministry of Trade, Industry and Energy (MOTIE, Korea).

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    Authors and Affiliations

    1. Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdaero, Giheung-gu, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea

      Juan Du, Hina Singh, KyungHwa Won, Jung-Eun Yang, Shahina Akter & Tae-Hoo Yi

    2. College of Bio and Food Technology, Dalian Polytechnic University, Qinggong-yuan No. 1 Ganjingzi-qu, Dalian, 116034, People’s Republic of China

      Feng-Xie Jin

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    1. Juan Du
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    2. Hina Singh
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    3. KyungHwa Won
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    4. Jung-Eun Yang
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    5. Shahina Akter
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    7. Tae-Hoo Yi
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    Corresponding author

    Correspondence to Tae-Hoo Yi.

    Additional information

    Communicated by Erko Stackebrandt.

    Juan Du and Hina Singh have equally contributed to this work.

    The NCBI GenBank accession number for the 16S rRNA gene sequence of strain THG-MM5T is KM658502.

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    Fig. S1

    Maximum-likelihood tree based on 16S rRNA gene sequence analysis showing phylogenetic relationships between strain THG-MM5T and related members of the genus Sphingomonas. Numbers at nodes represent percentages of bootstrap support based on a maximum-likelihood analysis of 1000 resampled datasets (PDF 16 kb)

    Fig. S2

    Two-dimensional TLC of the total polar lipids of THG-MM5T. a and b: Total lipids were detected by spraying with 5 % molybdophosphoric acid for strain THG-MM5T and S. sediminicola KACC 15039T, respectively; c: aminolipids revealed by α-naphthol–sulfuric acid; d: phospholipids detected by spraying with 0.2 % ninhydrin; e: glycolipids detected by spraying with molybdenum blue. Abbreviations: sphingoglycolipid (SGL), phosphatidylethanolamine (PE), diphosphatidylglycerol (DPE), phosphatidylglycerol (PG), phosphatidylcholine (PC), unidentified lipids (L1–2), unidentified glycolipid (GL1–2) and unidentified aminophospholipids (APL) (PDF 102 kb)

    Table 1

    The negative (−) properties of API ZYM and API 20NE tests of THG-MM5T (PPTX 53 kb)

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    Du, J., Singh, H., Won, K. et al. Sphingomonas flavus sp. nov. isolated from road soil. Arch Microbiol 197, 883–888 (2015). https://doi.org/10.1007/s00203-015-1123-2

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    • DOI: https://doi.org/10.1007/s00203-015-1123-2

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