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Paenibacillus ginsengiterrae sp. nov., a ginsenoside-hydrolyzing bacteria isolated from soil of ginseng field

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Abstract

A novel bacterial strain DCY89T was isolated from soil sample of ginseng field and was characterized using a polyphasic approach. Cells were Gram-reaction-positive, rod-shaped, spore-forming and motile with flagella. The strain was aerobic, esculin and starch positive, catalase- and oxidase-negative, optimum growth temperature, and pH were 25–30 °C and 6.0–7.5, respectively. On the basis of 16S rRNA gene sequence analysis, strain DCY89T was shown to belong to the genus Paenibacillus and the closest phylogenetic relatives were Paenibacillus cellulosilyticus KACC 14175T (98.2%), Paenibacillus kobensis KACC 15273T (98.1%), Paenibacillus xylaniclasticus KCTC 13719T (96.9%), and Paenibacillus curdlanolyticus KCTC 3759T (96.64%). The DNA G+C content was 52.5 mol%, and the predominant respiratory quinone was MK-7. The major fatty acids were iso-C15:0, iso-C16:0, and anteiso-C15:0. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol. The results of the genotypic analysis in combination with chemotaxonomic and physiological data demonstrated that DCY89T represented a novel species within the genus Paenibacillus, for which we propose the name Paenibacillus ginsengiterrae. The type strain is DCY89T (JCM 19887T = KCTC 33430T).

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Acknowledgments

This research was supported by Korea Institute of Planning & Evaluation for Technology in Food, Agriculture, Forestry & Fisheries (KIPET NO: 309019-03-3-SB010) and Next-Generation BioGreen 21 Program (SSAC, Grant#: PJ009529032014), Republic of Korea.

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Authors and Affiliations

  1. Graduate School of Biotechnology and Ginseng Bank, College of Life Science, Kyung Hee University, Seocheon-dong, Giheung-gu, Yongin-Si, Gyeonggi-do, 446-701, Republic of Korea

    Md. Amdadul Huq, Yeon-Ju Kim, Muhammad Zubair Siddiqi & Deok-Chun Yang

  2. Department of Oriental Medicinal Materials and Processing, College of Life Science, Kyung Hee University, Yongin, 449-701, Republic of Korea

    Yeon-Ju Kim & Van-An Hoang

Authors
  1. Md. Amdadul Huq
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  2. Yeon-Ju Kim
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  3. Van-An Hoang
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  4. Muhammad Zubair Siddiqi
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  5. Deok-Chun Yang
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Corresponding authors

Correspondence to Yeon-Ju Kim or Deok-Chun Yang.

Additional information

Communicated by Erko Stackebrandt.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain DCY89T is KF915799.

Electronic supplementary material

Below is the link to the electronic supplementary material.

203_2014_1073_MOESM1_ESM.tif

Two-dimensional TLC of the total polar lipids of strain P. ginsengiterrae DCY89T (A) and P. cellulosilyticus KACC 14175T (B), stained for total polar lipids with 5% ethanolic molybdophosphoric acid. Abbreviations: DPG, diphosphatidylglycerol; PE, phosphatidylethanolamine; PG, phosphatidylglycerol. (TIFF 18,421kb)

Transmission electron micrograph of P. ginsengiterrae DCY89T. Bar, 0.5μm (TIFF 3,270kb)

Spore-formation by P. ginsengiterrae DCY89T. (TIFF 2,258 kb)

203_2014_1073_MOESM4_ESM.pptx

The maximum-likelihood (ML) tree based on 16S rRNA gene sequence analysis showing phylogenetic relationships of strain DCY89T and members of the genus Paenibacillus. (PPTX 89kb)

203_2014_1073_MOESM5_ESM.tif

Time-course TLC analysis of metabolite of ginsenoside Rb1 by P. ginsengiterrae DCY89T. C, control; S, saponin standards (TIFF 390kb)

203_2014_1073_MOESM6_ESM.tif

HPLC profiles of metabolite of ginsenoside Rb1 converted by P. ginsengiterrae DCY89T. A, ginsenoside standards; B, ginsenoside Rb1 control and C, ginsenoside Rb1 metabolite. (TIFF 977kb)

203_2014_1073_MOESM7_ESM.tif

Mass spectra of ginsenoside Rb1 after hydrolysis by P. ginsengiterrae DCY89T. (A) Mass spectrum of standard ginsenoside Rd; (B) Mass spectrum of bioconverted ginsenoside Rd. (TIFF 1,507kb)

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Huq, M.A., Kim, YJ., Hoang, VA. et al. Paenibacillus ginsengiterrae sp. nov., a ginsenoside-hydrolyzing bacteria isolated from soil of ginseng field. Arch Microbiol 197, 389–396 (2015). https://doi.org/10.1007/s00203-014-1073-0

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