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Proteomic profiling of Botrytis cinerea conidial germination

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Abstract

Botrytis cinerea is one of the most relevant plant pathogenic fungi. The first step during its infection process is the germination of the conidia. Here, we report on the first proteome analysis during the germination of B. cinerea conidia, where 204 spots showed significant differences in their accumulation between ungerminated and germinated conidia by two-dimensional polyacrylamide gel electrophoresis and qPCR. The identified proteins were grouped by gene ontology revealing that the infective tools are mainly preformed inside the ungerminated conidia allowing a quick fungal development at the early stages of conidial germination. From 118 identified spots, several virulence factors have been identified while proteins, such as mannitol-1-phosphate dehydrogenase, 6,7-dimethyl-8-ribityllumazine synthase or uracil phosphoribosyltransferase, have been disclosed as a new potential virulence factors in botrytis whose role in pathogenicity needs to be studied to gain new insights about the role of these proteins as therapeutic targets and virulence factors.

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Abbreviations

SDW:

Sterile distilled water

HPI:

Hour post inoculation

GO:

Gene ontology

ID:

Identification

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Acknowledgments

We thank the Prof. Dr. Tudzynski for donated the strain B. cinerea B05.10 used in this study. This research has been financed by the Spanish Government DGICYT—AGL2009-13359-CO2/AGR and AGL2012-39798-C02-02 (www.micinn.es/portal/site/MICINN/), by the Andalusian Government (Junta de Andalucía, PO7-FQM-002689; www.juntadeandalucia.es/innovacioncienciayempresa), and by the CeiA3 International Campus of excellence in Agrifood (18INACO177.002AA; http://www.uco.es/cei-A3/). Victoria E. González-Rodríguez was supported by the grant FPU of the Ministerio de Educación, Government of Spain (AP2009-1309). Eva Liñeiro was supported by a FPI grant from the University of Cadiz (2010-152).

Conflict of interest

The authors have declared no conflict of interest.

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Authors and Affiliations

  1. Laboratory of Microbiology, Marine and Environmental Sciences Faculty, Andalusian Center for Grape and Grapevine Research, CeiA3 International Campus of Excellence in Agrifood, University of Cádiz, Pol. Río San Pedro s/n, 11510, Puerto Real, Cádiz, Spain

    Victoria E. González-Rodríguez, Eva Liñeiro, Carlos Garrido, Jesús Manuel Cantoral & Francisco Javier Fernández-Acero

  2. Max Planck Institute for Plant Breeding Research, MS Group, Carl-von-Linné-Weg 10, 50829, Cologne, Germany

    Thomas Colby, Anne Harzen & Jürgen Schmidt

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  1. Victoria E. González-Rodríguez
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  2. Eva Liñeiro
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Corresponding author

Correspondence to Francisco Javier Fernández-Acero.

Additional information

Communicated by Erko Stackebrandt.

Victoria E. González-Rodríguez and Eva Liñeiro have contributed equally to this work.

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González-Rodríguez, V.E., Liñeiro, E., Colby, T. et al. Proteomic profiling of Botrytis cinerea conidial germination. Arch Microbiol 197, 117–133 (2015). https://doi.org/10.1007/s00203-014-1029-4

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