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Bacillus thermophilum sp. nov., isolated from a microbial fuel cell

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Abstract

A novel thermophilic, Gram-staining positive bacterium, designated DX-2T, was isolated from the anode biofilm of a microbial fuel cell. Cells of the strain were oxidase positive, catalase positive, facultative anaerobic, motile rods. The isolate grew at 30–60 °C (optimum 50 °C) and pH 5–9 (optimum pH 8–8.5). The pairwise 16S rRNA gene sequence similarities showed that strain DX-2T was most closely related to Bacillus fumarioli LMG 17489T (96.2 %), B. firmus JCM 2512T (96.0 %) and B. foraminis DSM 19613T (95.7 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DX-2T formed a cluster with B. smithii (95.5 %) and B. infernus (94.9 %). The genomic G+C content of DX-2T was 43.7 mol%. The predominant respiratory quinone was MK-7. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and unknown phospholipids. The major cellular fatty acid was iso-C16:0. Based on its phenotypic characteristics, chemotaxonomic features, and results of phylogenetic analysis, the strain was identified to represent a distinct novel species in the genus Bacillus, and the name proposed is B. thermophilum sp. nov. The type strain is DX-2T (=CCTCC AB2012194T = KCTC 33128T).

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Acknowledgments

This work was supported by the Key Projects of the National Science & Technology Pillar Program of China (2012BAD14B16) and the Science and Technology Planning Project of Guangdong Province, China (2011B030900003, 2011A090200038 and 2011A030600003).

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Authors and Affiliations

  1. Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou, 510650, People’s Republic of China

    Jia Tang, Guiqin Yang, Junlin Wen, Zhen Yu & Shungui Zhou

  2. College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, People’s Republic of China

    Zhi Liu

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  1. Jia Tang
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  2. Guiqin Yang
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  3. Junlin Wen
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  4. Zhen Yu
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  5. Shungui Zhou
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  6. Zhi Liu
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Corresponding authors

Correspondence to Shungui Zhou or Zhi Liu.

Additional information

Communicated by Erko Stackebrandt.

Jia Tang and Guiqin Yang contributed equally to this work.

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Tang, J., Yang, G., Wen, J. et al. Bacillus thermophilum sp. nov., isolated from a microbial fuel cell. Arch Microbiol 196, 629–634 (2014). https://doi.org/10.1007/s00203-014-1001-3

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  • DOI: https://doi.org/10.1007/s00203-014-1001-3

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