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Repression of oxalic acid-mediated mineral phosphate solubilization in rhizospheric isolates of Klebsiella pneumoniae by succinate

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Abstract

Two strains of Klebsiella (SM6 and SM11) were isolated from rhizospheric soil that solubilized mineral phosphate by secretion of oxalic acid from glucose. Activities of enzymes for periplasmic glucose oxidation (glucose dehydrogenase) and glyoxylate shunt (isocitrate lyase and glyoxylate oxidase) responsible for oxalic acid production were estimated. In presence of succinate, phosphate solubilization was completely inhibited, and the enzymes glucose dehydrogenase and glyoxylate oxidase were repressed. Significant activity of isocitrate lyase, the key enzyme for carbon flux through glyoxylate shunt and oxalic acid production during growth on glucose suggested that it could be inducible in nature, and its inhibition by succinate appeared to be similar to catabolite repression.

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Abbreviations

C:

Carbon

N:

Nitrogen

P:

Phosphorus

MPS:

Mineral phosphate solubilization

PSMs:

Phosphate solubilizing microorganisms

PQQ:

Pyrroloquinoline quinone

GDH:

Glucose dehydrogenase

GO:

Glyoxylate oxidase

ICL:

Isocitrate lyase

TRP:

Tris rock phosphate

SMCR:

Succinate-mediated catabolite repression

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Authors and Affiliations

  1. Institute of Science, Nirma University, Sarkhej-Gandhinagar Highway, Ahmedabad, 382481, Gujarat, India

    Mahendrapal Singh Rajput & Shalini Rajkumar

  2. Molecular Microbial Biochemistry Laboratory, Department of Biochemistry, Faculty of Science, Maharaja Sayajirao University of Baroda, Vadodara, 390002, Gujarat, India

    G. Naresh Kumar

Authors
  1. Mahendrapal Singh Rajput
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  2. G. Naresh Kumar
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  3. Shalini Rajkumar
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Correspondence to Shalini Rajkumar.

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Communicated by John Helmann.

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Rajput, M.S., Naresh Kumar, G. & Rajkumar, S. Repression of oxalic acid-mediated mineral phosphate solubilization in rhizospheric isolates of Klebsiella pneumoniae by succinate. Arch Microbiol 195, 81–88 (2013). https://doi.org/10.1007/s00203-012-0850-x

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  • DOI: https://doi.org/10.1007/s00203-012-0850-x

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