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Pannonibacter indica sp. nov., a highly arsenate-tolerant bacterium isolated from a hot spring in India

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Abstract

A novel aerobic bacterium, strain HT23T, able to grow on 500 mM sodium arsenate was isolated from a hot-spring sediment sample collected from Athamallik, Orissa, India. Cells of this isolate were Gram negative. Heterotrophic growth was observed at pH 6.0–11.0 and 20–45 °C. Optimum growth was observed at 37 °C and pH 7.0–10.0. The major polar lipids are diphosphatidyl glycerol, phosphatidyl glycerol, phosphatidyl ethanolamine, phosphatidyl choline and phosphatidyl monomethyl ethanolamine. The major isoprenoid quinone was Q-10. 16S rRNA gene sequence analysis indicated that the bacterium clustered with the genus Pannonibacter and showed 98.9 % similarity with Pannonibacter phragmitetus C6-19T (DSM 14782T) and 98 % with the P. phragmitetus group B and P. phragmitetus group E strains. Levels of DNA–DNA relatedness between the strain HT23T and P. phragmitetus C6-19T (DSM 14782T) and other strains of P. phragmitetus group B and group E strains were below 55 %. On the basis of phenotypic and chemotaxonomic characteristics, 16S rRNA gene sequence analysis and DNA–DNA hybridization data, strain HT23T is considered to represent a novel species of the genus Pannonibacter, for which the name Pannonibacter indica sp. nov. is proposed. The type strain is HT23T (=JCM 16851T = DSM 23407T = LMG 25769T).

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Acknowledgments

We are grateful to the All India Institute of Medical Sciences, New Delhi, for electron microscopic facility. Pannonibacter phragmitetus C6-19T (DSM 14782T) and the representative of Pannonibacter phragmitetus group B strains (LMG 5410, LMG 5411, LMG 5412 and LMG 5421) and Pannonibacter phragmitetus group E strains (LMG 5430 and LMG 5431) used in this study were provided by the DSMZ, Braunschweig, Germany and the BCCM/LMG Bacteria collection, Universiteit Gent, Belgium. This work was supported by the Department of Biotechnology, Ministry of Science and Technology, Govt. of India. The author, S. Bandyopadhyay, acknowledges the Council of Scientific and Industrial Research (CSIR), Government of India, New Delhi, for providing the research fellowship.

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Authors and Affiliations

  1. Department of Biotechnology, Institute of Life Sciences, Nalco Square, Bhubaneswar, 751 023, India

    Saumya Bandyopadhyay & Subrata K. Das

  2. Leibniz-Institut DSMZ-Deutsche Sammlung von Mikro-organismen und Zellkulturen GmbH, Inhoffenstraße 7B, 38124, Braunschweig, Germany

    Peter Schumann

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  1. Saumya Bandyopadhyay
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  2. Peter Schumann
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  3. Subrata K. Das
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Correspondence to Subrata K. Das.

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Communicated by Erko Stackebrandt.

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Bandyopadhyay, S., Schumann, P. & Das, S.K. Pannonibacter indica sp. nov., a highly arsenate-tolerant bacterium isolated from a hot spring in India. Arch Microbiol 195, 1–8 (2013). https://doi.org/10.1007/s00203-012-0840-z

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