Abstract
The nanchangmycin (NAN) produced by Streptomyces nanchangensis NS3226 is a polyether antibiotic resembling monensin in their gene clusters and the chemical structures. They can inhibit gram-positive bacteria and be a growth promoter for ruminants. Within the nanchangmycin gene cluster (nan), we identified that two SARP-family regulatory genes, nanR1 and nanR2, were both required to activate the transcription of all nan polyketide genes. Overexpression of NanR1 and NanR2 in wild-type increase NAN yields by at least three folds. Bioinformatic analysis of the immediate upstream DNA sequence of each nan gene and quantitative real-time RT-PCR analysis of the nan operons identified five putative SARP binding sites. Moreover, deletion of an AraC-family repressor gene nanR4 increased expression of NanR1 and R2 and led to a threefold increase in NAN production.
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Acknowledgments
Authors gave their thanks to Tobias Kieser for his helpful comment and manuscript editing. This work was supported by grants from the Ministry of Science and Technology (973 and 863 Programs), the National Science Foundation of China, the Ministry of Education, and the Science and Technology Commission of Shanghai Municipality.
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Communicated by Erko Stackebrandt.
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Yu, Q., Du, A., Liu, T. et al. The biosynthesis of the polyether antibiotic nanchangmycin is controlled by two pathway-specific transcriptional activators. Arch Microbiol 194, 415–426 (2012). https://doi.org/10.1007/s00203-011-0768-8
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DOI: https://doi.org/10.1007/s00203-011-0768-8