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Response to different oxidants of Saccharomyces cerevisiae ure2Δ mutant

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Abstract

Growth of Saccharomyces cerevisiae ure2Δ mutant strain was investigated in the presence of diverse oxidant compounds. The inability of the strain to grow on a medium supplemented with H2O2 was confirmed and a relationship between diminishing levels of glutathione (GSH) and peroxide sensitivity was established. Data for the lack of significant effect of URE2 disruption on the cellular growth in the presence of paraquat and menadione were obtained. The possible role of Ure2p in acquiring sensitivity to oxidative stress by means of its regulatory role in the GATA signal transduction pathway was discussed. It was suggested that the susceptibility of ure2Δ mutant to the exogenous hydrogen peroxide can result from increased GSH degradation due to the deregulated localization of the γ-glutamyl transpeptidase activating factors Gln3/Gat1. The important role of Ure2p in in vivo glutathione-mediated reactive oxygen species (ROS) scavenging was shown by measuring the activity of antioxidant enzymes glutathione peroxidase, superoxide dismutase (SOD) and catalase in an URE2 disrupted strain. A time-dependent increase in SOD and catalase activity was observed. More importantly, it was shown that the ure2 mutation could cause significant disturbance in cellular oxidant balance and increased ROS level.

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Acknowledgments

We thank Ivan Tarassov for providing the p413 vector and to Ales Vancura for pET23b-URE2 and pET23b-URE2(ΔN) plasmids. This work was supported by a CNRS ATIP grant to S.V., by a grant from National Science Fund of Bulgarian Ministry of Education and Science (Project Б-BУ-201/06) to A.K., and by a visiting scientist grant of the European Doctoral College of Strasbourg to A.K. T.T. was the recipient of an Agence Universitaire de la Francophonie PhD grant, and was a member of the European Doctoral College of Strasbourg.

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Authors and Affiliations

  1. Department “General and Industrial Microbiology”, Faculty of Biology, The Sofia University “St. Kliment Ohridski”, 8 Dragan Tsankov Street, 1164, Sofia, Bulgaria

    Ventsislava Y. Petrova & Anna V. Kujumdzieva

  2. University of Strasbourg UMR 7156 CNRS, 28 rue Goethe, 67083, Strasbourg Cedex, France

    Tatina T. Todorova & Stéphane Vuilleumier

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  1. Tatina T. Todorova
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  2. Ventsislava Y. Petrova
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  3. Stéphane Vuilleumier
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  4. Anna V. Kujumdzieva
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Correspondence to Anna V. Kujumdzieva.

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Communicated by Axel Brakhage.

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Todorova, T.T., Petrova, V.Y., Vuilleumier, S. et al. Response to different oxidants of Saccharomyces cerevisiae ure2Δ mutant. Arch Microbiol 191, 837–845 (2009). https://doi.org/10.1007/s00203-009-0512-9

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  • DOI: https://doi.org/10.1007/s00203-009-0512-9

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