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Amiodarone inhibits multiple drug resistance in yeast Saccharomyces cerevisiae

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Abstract

Amiodarone is a widely used antiarrhythmic drug. There is also evidence that amiodarone decreases multidrug resistance in human cell lines. In this paper, we have shown that amiodarone has similar effect on yeast, Saccharomyces cerevisiae, decreasing multiple drug resistance. Amiodarone stimulates the accumulation of ethidium bromide by inhibiting its efflux from the cells. The effect of amiodarone is much stronger on wild-type cells compared to the mutant with inactivated ABC-transporters. Interestingly, the action of amiodarone is additive with the one of chloroquine, a known inhibitor of ABC-transporters. We speculate that these findings could help in the development of antifungal drug mixes.

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Abbreviations

ABC:

ATP-binding cassette

MDR:

Multiple drug resitance

EB:

Ethidium bromide

C12-TPP:

Dodecyltriphenylphosphonium

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Authors and Affiliations

  1. A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, GSP-2, Leninskie Gory, 119992, Moscow, Russia

    Dmitry A. Knorre, Tatiana N. Krivonosova, Olga V. Markova & Fedor F. Severin

Authors
  1. Dmitry A. Knorre
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  2. Tatiana N. Krivonosova
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  3. Olga V. Markova
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  4. Fedor F. Severin
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Corresponding author

Correspondence to Dmitry A. Knorre.

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Communicated by Axel Brakhage.

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Knorre, D.A., Krivonosova, T.N., Markova, O.V. et al. Amiodarone inhibits multiple drug resistance in yeast Saccharomyces cerevisiae . Arch Microbiol 191, 675–679 (2009). https://doi.org/10.1007/s00203-009-0493-8

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  • DOI: https://doi.org/10.1007/s00203-009-0493-8

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