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Chlamydia pneumoniae and osteoporosis-associated bone loss: a new risk factor?

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Abstract

Summary

We found an association between the presence of Chlamydia pneumoniae DNA both in osteoporotic bone tissue and peripheral blood mononuclear cells (PBMCs) and the increase in circulating resorptive cytokines.

Introduction

Our study was designed to determine whether C. pneumoniae infection may be involved in osteoporosis-associated bone loss.

Methods

The study included 59 women undergoing hip joint replacement surgery for femoral neck fracture: 32 with osteoporosis and 27 with osteoarthritis. A total of 118 tissue specimens (59 bone tissues, 59 PBMCs) were examined for C. pneumoniae DNA by real-time polymerase chain reaction (PCR). Serum levels of soluble receptor activator of nuclear factor kappa B ligand (sRANKL), osteoprotegerin (OPG), interleukin (IL)-1β, tumor necrosis factor-α, and IL-6 were also measured.

Results

C. pneumoniae DNA was detected in osteoporotic bone tissue whereas it was not found in non-osteoporotic bone tissue (p < 0.05). A significantly higher rate of C. pneumoniae DNA (p < 0.05) was found in PBMCs of osteoporotic patients than in those of osteoarthritis patients. Among osteoporotic patients, serum sRANKL, IL-1, and IL-6 concentrations as well as sRANKL/OPG ratio significantly differ between patients with bone tissue and PBMCs positive to C. pneumoniae and C. pneumoniae-negative patients.

Conclusion

The association between the presence of C. pneumoniae DNA, both in bone tissue and PBMCs, and the increase in sRANKL/OPG ratio as well as in IL-1β and IL-6 levels observed in osteoporotic patients suggests C. pneumoniae infection as a new risk factor for osteoporosis.

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References

  1. Rachner TD, Khosla S, Hofbauer LC (2011) Osteoporosis: now and the future. Lancet 377:1276–1287

    Article  PubMed  CAS  Google Scholar 

  2. Brewer L, Williams D, Moore A (2011) Current and future treatment options in osteoporosis. Eur J Clin Pharmacol 67:321–331

    Article  PubMed  CAS  Google Scholar 

  3. Claro T, Widaa A, O’Seaghdha M, Miajlovic H, Foster TJ, O’Brien FJ, Kerrigan SW (2011) Staphylococcus aureus protein A binds to osteoblasts and triggers signals that weaken bone in osteomyelitis. PLoS One 6:e18748

    Article  PubMed  CAS  Google Scholar 

  4. Schreiner H, Markowitz K, Miryalkar M, Moore D, Diehl S, Fine DH (2011) Aggregatibacter actinomycetemcomitans-induced bone loss and antibody response in three rat strains. J Periodontol 82:142–150

    Article  PubMed  Google Scholar 

  5. Delpino MV, Fossati CA, Baldi PC (2009) Proinflammatory response of human osteoblastic cell lines and osteoblast-monocyte interaction upon infection with Brucella spp. Infect Immun 77:984–995

    Article  PubMed  CAS  Google Scholar 

  6. Graves D (2008) Cytokines that promote periodontal tissue destruction. J Periodontol 79:1585–1591

    Article  PubMed  CAS  Google Scholar 

  7. Marriott I (2004) Osteoblast responses to bacterial pathogens: a previously unappreciated role for bone-forming cells in host defense and disease progression. Immunol Res 30:291–308

    Article  PubMed  CAS  Google Scholar 

  8. Bailey L, Engström P, Nordström A, Bergström S, Waldenström A, Nordström P (2008) Chlamydia pneumoniae infection results in generalized bone loss in mice. Microbes Infect 10:1175–1181

    Article  PubMed  CAS  Google Scholar 

  9. Rizzo A, Di Domenico M, Carratelli CR, Mazzola N, Paolillo R (2011) Induction of proinflammatory cytokines in human osteoblastic cells by Chlamydia pneumoniae. Cytokine 56:450–457

    Article  PubMed  CAS  Google Scholar 

  10. Di Pietro M, Tramonti A, De Santis F, De Biase D, Schiavoni G, Sessa R (2012) Analysis of gene expression in penicillin G induced persistence of Chlamydia pneumoniae. J Biological Regulators & Homeostatic Agents 26:277–284

    Google Scholar 

  11. Bellmann-Weiler R, Martinz V, Kurz K, Engl S, Feistritzer C, Fuchs D, Rupp J, Paldanius M, Weiss G (2010) Divergent modulation of Chlamydia pneumoniae infection cycle in human monocytic and endothelial cells by iron, tryptophan availability and interferon gamma. Immunobiology 215:842–848

    Article  PubMed  CAS  Google Scholar 

  12. Rosenfeld ME, Campbell LA (2011) Pathogens and atherosclerosis: update on the potential contribution of multiple infectious organisms to the pathogenesis of atherosclerosis. Thromb Haemost 106:858–867

    Article  PubMed  CAS  Google Scholar 

  13. Sessa R, Nicoletti M, Di Pietro M, Schiavoni G, Santino I, Zagaglia C, Del Piano M, Cipriani P (2009) Chlamydia pneumoniae and atherosclerosis: current state and future prospectives. Int J Immunopathol Pharmacol 22:9–14

    PubMed  CAS  Google Scholar 

  14. Gracey E, Inman RD (2011) Chlamydia-induced ReA: immune imbalances and persistent pathogens. Nat Rev Rheumatol 8:55–59

    Article  PubMed  Google Scholar 

  15. Villareal C, Whittum-Hudson JA, Hudson AP (2002) Persistent Chlamydiae and chronic arthritis. Arthritis Res 4:5–9

    Article  PubMed  Google Scholar 

  16. Netea MG, Selzmann CH, Kullberg BJ (2000) Acellular components of Chlamydia pneumoniae stimulate cytokine production in human blood mononuclear cells. Eur J Immunol 30:541–549

    Article  PubMed  CAS  Google Scholar 

  17. Takano R, Yamaguchi H, Sugimoto S, Nakamura S, Friedman H, Yamamoto Y (2005) Cytokine response of lymphocytes persistently infected with Chlamydia pneumoniae. Curr Microbiol 50:160–166

    PubMed  CAS  Google Scholar 

  18. Merkel KD, Erdmann JM, McHugh KP, Abu-Amer Y, Ross FP, Teitelbaum SL (1999) Tumor necrosis factor-α mediates orthopedic implant osteolysis. Am J Pathol 154:203–210

    Article  PubMed  CAS  Google Scholar 

  19. WHO Study Group (1994) Assessment of fracture risk and its application to screening for postmenopausal osteoporosis. Report of a WHO Study Group World Health Organ Tech Rep Ser 843:1–129

    Google Scholar 

  20. Sessa R, Di Pietro M, Schiavoni G, Petrucca A, Cipriani P, Zagaglia C, Nicoletti M, Santino I, del Piano M (2007) Measurement of Chlamydia pneumoniae bacterial load in peripheral blood mononuclear cells may be helpful to assess the state of chlamydial infection in patients with carotid atherosclerotic disease. Atherosclerosis 195:e224–e230

    Article  PubMed  CAS  Google Scholar 

  21. Gieffers J, van Zandbergen G, Rupp J, Sayk F, Krüger S, Ehlers S, Solbach W, Maass M (2004) Phagocytes transmit Chlamydia pneumoniae from the lungs to the vasculature. Eur Respir J 23:506–510

    Article  PubMed  CAS  Google Scholar 

  22. Gérard HC, Schumacher HR, El-Gabalawy H, Goldbach-Mansky R, Hudson AP (2000) Chlamydia pneumoniae present in the human synovium are viable and metabolically active. Microb Pathog 29:17–24

    Article  PubMed  Google Scholar 

  23. Carter JD, Gérard HC, Espinoza LR, Ricca LR, Valeriano J, Snelgrove J, Oszust C, Vasey FB, Hudson AP (2009) Chlamydiae as etiologic agents in chronic undifferentiated spondylarthritis. Arthritis Rheum 60:1311–1316

    Article  PubMed  Google Scholar 

  24. Teti A (2011) Bone development: overview of bone cells and signaling. Curr Osteoporos Rep 9:264–273

    Article  PubMed  Google Scholar 

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Acknowledgments

Funding for this study was provided by the Center for Social Disease Research, “Sapienza” University, Rome to R. Sessa.

Conflicts of interest

None.

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Authors and Affiliations

  1. Department of Public Health and Infectious Diseases, “Sapienza” University, P.le Aldo Moro 5, 00185, Rome, Italy

    M. Di Pietro, G. Schiavoni & R. Sessa

  2. Department of Orthopaedics, San Giovanni Calibita-Fatebenefratelli Hospital, Isola Tiberina,, Rome, Italy

    V. Sessa

  3. Department of General Surgery, San Camillo Forlanini Hospital, Rome, Italy

    F. Pallotta

  4. Department of Anatomical, Histological, Forensic Medicine and Orthopedics Sciences, “Sapienza” University, Rome, Italy

    G. Costanzo

Authors
  1. M. Di Pietro
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  2. G. Schiavoni
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  3. V. Sessa
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  4. F. Pallotta
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  5. G. Costanzo
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  6. R. Sessa
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Corresponding author

Correspondence to R. Sessa.

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Di Pietro, M., Schiavoni, G., Sessa, V. et al. Chlamydia pneumoniae and osteoporosis-associated bone loss: a new risk factor?. Osteoporos Int 24, 1677–1682 (2013). https://doi.org/10.1007/s00198-012-2217-1

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  • DOI: https://doi.org/10.1007/s00198-012-2217-1

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