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Molekulargenetische Identifikation von Canidae in der forensischen Praxis

Molecular genetic identification of Canidae in the forensic practice

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Zusammenfassung

Die Zahl der Straftaten mit Tieren als „Tatwaffe“ bzw. als Opfer nimmt stetig zu. Um den daraus resultierenden Fragestellungen, wie z. B. der molekulargenetischen Identitätsbestimmung, gerecht zu werden, müssen in der forensischen DNA-Analytik entsprechende molekulargenetische sowie biostatistische Methoden entwickelt und validiert werden. Im vorgestellten Fall konnte die Identität eines Hundes im Fall einer schweren Tierquälerei mit resultierender Todesfolge nachgewiesen und der Täter so überführt werden. Hierzu wurden verschiedene Vortests an den asservierten Spuren, eine selbst entwickelte Restriktionsfragmentlängenpolymorphismus(RFLP)-Analyse, eine kommerziell erhältliche „Short-tandem-repeats“(STR)-Bestimmung und die anschließende biostatistische Berechnung mithilfe einer selbst entwickelten Software durchgeführt.

Abstract

The number of offences with animals as a “weapon” or as victims is steadily increasing; therefore, the establishment of molecular genetic animal-specific assays is recommended and one goal was to develop a simple genetic testing system for species determination. In addition to forensic genetic tests, biostatistical methods must be developed and validated. This article presents a case where a dog was beaten to death. Employing preliminary tests for blood determination, a self-designed restriction fragment length polymorphism (RFLP) analysis, short tandem repeat (STR) typing and subsequent biostatistical calculations using a self-developed software program, the identity of the dead dog could be proven and the animal could be linked to the potential weapon leading to the conviction of the offender.

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Correspondence to T. Schwark.

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J.-H. Modrow, O. Revyakina, H. Reder, J. Hasse, N. von Wurmb-Schwark und T. Schwark geben an, dass kein Interessenkonflikt besteht.

Dieser Beitrag beinhaltet keine Studien an Menschen oder Tieren.

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Modrow, JH., Revyakina, O., Reder, H. et al. Molekulargenetische Identifikation von Canidae in der forensischen Praxis. Rechtsmedizin 26, 42–46 (2016). https://doi.org/10.1007/s00194-015-0036-3

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  • DOI: https://doi.org/10.1007/s00194-015-0036-3

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