Abstract
Optical coherence tomography (OCT) is a modern technique that allows for in vivo, fast, high-resolution 3D imaging. OCT can be efficiently used in eye research and diagnostics, when retinal images are processed to extract borders of retinal layers. In this paper, we present two novel algorithms for delineation of three main borders in rodent retinal images. The first, fast algorithm is based on row projections in a sliding window. It provides initial borders for a slower but more precise variational algorithm that iteratively refines the borders. The results obtained by the two algorithms are quantitatively evaluated by comparison to the borders manually extracted in a set of retinal images.
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Cabrera Fernández D., Salinas H.M., Puliafito C.A.: Automated detection of retinal layer structures on optical coherence tomography images. Optics Express 13(25), 10200–10216 (2005)
Caselles V., Catté F., Coll T., Dibos F.: A geometric model for active contours in image processing. Numer. Math. 66(1), 1–31 (1993)
Caselles V., Kimmel R., Sapiro G.: Geodesic active contours. Int. J. Comput. Vision 22(1), 61–79 (1997)
Chan T.F., Vese L.A.: Contours without edges. IEEE Trans. Image Process. 10(2), 266–277 (2001)
Fabritius T. et al.: Automated segmentation of the macula by optical coherence tomography. Optics Express 17(18), 15659–15669 (2009)
Faugeras, O., Gomes, J., Keriven, R.: Variational principles in computational stereo. In: Geometric Level Set Methods in Imaging, Vision and Graphics. Springer, Berlin (2003)
Faugeras, O., Keriven, R.: Variational principles, surface evolution, PDE-s, level set methods, and the stereo problem. Technical report 3021, INRIA (1996)
Faugeras O., Keriven R.: Variational principles, surface evolution, PDE-s, level set methods, and the stereo problem. IEEE Trans. Image Process. 7, 336–344 (1998)
Fuller A.R. et al.: Segmentation of three-dimensional retinal image data. IEEE Trans. Visual. Comput. Graph. 13(6), 1719–1726 (2007)
Garvin M.K. et al.: Intraretinal layer segmentation of macular optical coherence tomography images using optimal 3-D graph search. IEEE Trans. Med. Imaging 27(10), 1495–1505 (2008)
Kajić V. et al.: Robust segmentation of intraretinal layers in the normal human fovea using a novel statistical model based on texture and shape analysis. Optics Express 18(14), 14730–14744 (2010)
Kass M., Witkin A., Terzopoulos D.: Snakes: Active contour models. Int. J. Comput. Vision 1(4), 321–331 (1988)
Leitgeb, R.A.: Paradigm shifts in optical coherence tomography. In: Proceedings of SPIE, vol. 6616, 661604 (2007)
Mishra A., Wong A., Bizheva K., Clausi D.A.: Intra-retinal layer segmentation in optical coherence tomography images. Optics Express 17(26), 23719–23728 (2009)
Osher S., Fedkiw R.: Level Set Methods and Dynamic Implicit Surfaces. Springer, Berlin (2003)
Puvanathasan P., Bizheva K.: Speckle noise reduction algorithm for optical coherence tomography based on interval type II fuzzy set. Optics Express 15, 15747–15758 (2007)
Ruggeri M. et al.: In vivo three-dimensional high-resolution imaging of rodent retina with spectral-domain optical coherence tomography. Invest. Ophthalmol. Visual Sci. 48(4), 1808 (2007)
Ruggeri M. et al.: Retinal tumor imaging and volume quantification in mouse model using spectral-domain optical coherence tomography. Optics Express 17(5), 4074 (2009)
Salinas H.M., Fernández D.C.: Comparison of PDE-based nonlinear diffusion approaches for image enhancement and denoising in optical coherence tomography. IEEE Trans. Med. Imaging 26(6), 761–771 (2007)
Sethian J.A.: Level Set Methods and Fast Marching Methods. Cambridge University Press, Cambridge (2002)
Shah D.M.J.: Optimal approximation by piecewise smooth functions and associated variational problems. Commun. Pure Appl. Math. 17, 577–685 (1989)
Szkulmowski M. et al.: Analysis of posterior retinal layers in spectral optical coherence tomography images of the normal retina and retinal pathologies. J. Biomed. Optics 12, 041207 (2007)
Wong A., Mishra A., Bizheva K., Clausi D.A.: General Bayesian estimation for speckle noise reduction in optical coherence tomography retinal imagery. Optics Express 18(8), 8338–8352 (2010)
Yazdanpanah, A., Hamarneh, G., Smith, B., Sarunic M.: Intra-retinal layer segmentation in optical coherence tomography using an active contour approach. Medical image computing and computer-assisted intervention, MICCAI 2009, pp. 649–656 (2009)
Zawadzki R.J. et al.: Adaptation of a support vector machine algorithm for segmentation and visualization of retinal structures in volumetric optical coherence tomography data sets. J. Biomed. Optics 12, 041206 (2007)
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Molnár, J., Chetverikov, D., Cabrera DeBuc, D. et al. Layer extraction in rodent retinal images acquired by optical coherence tomography. Machine Vision and Applications 23, 1129–1139 (2012). https://doi.org/10.1007/s00138-011-0343-y
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DOI: https://doi.org/10.1007/s00138-011-0343-y