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Anti-oxidative Responses on Hepatic Tissue of Zebrafish (Danio rerio) in a Short Duration of Sub-lethal Concentrations of Cadmium Exposure

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Abstract

The aim of the present study was to identify whether the responses of oxidative stress in zebrafish liver are similar to those in mammalians upon low doses of Cd2+ exposure in short durations. Fish were exposed to 1.78 μM Cd2+ (treatment) and 0.0 μM Cd2+ (control) for 0, 1, 3, and 6 h. The reactive oxygen species (ROS) and lipid peroxidation (LPO) of hepatic tissues significantly increased after 3 and 6 h of Cd2+ exposure, respectively. Antioxidants glutathione peroxidase (gpx1a), superoxide dismutase (sod), and catalase (cat) were up regulated after 1–3 h, and metallothionein isoforms (smtB and mt2) increased after 3–6 h of Cd2+ exposure. The caspase-3 and p53 mRNA expressions significantly increased threefolds after 1 h of Cd2+ exposure. Results confirmed that oxidative stress in the hepatic tissue was induced by Cd2+ within 3 h. However, anti-oxidative functions immediately up regulated, causing cell apoptosis levels to decrease after 6 h of Cd2+ exposure.

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Acknowledgements

This work was supported by grants (NSC101-2311-B-415-001-MY3) from the National Science Council, and in part supported by National Chiayi University, Taiwan.

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Authors and Affiliations

  1. Department of Aquatic Biosciences, National Chiayi University, Chiayi, 600, Taiwan, Republic of China

    Su Mei Wu, Li-Hsin Shu & Jia-Hao Liu

  2. Graduate Institute of Biomedical and Biopharmaceutical Sciences, National Chiayi University, Chiayi, 600, Taiwan, Republic of China

    Ching Hsein Chen

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  1. Su Mei Wu
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  2. Li-Hsin Shu
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  3. Jia-Hao Liu
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  4. Ching Hsein Chen
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Correspondence to Su Mei Wu.

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Wu, S.M., Shu, LH., Liu, JH. et al. Anti-oxidative Responses on Hepatic Tissue of Zebrafish (Danio rerio) in a Short Duration of Sub-lethal Concentrations of Cadmium Exposure. Bull Environ Contam Toxicol 98, 612–618 (2017). https://doi.org/10.1007/s00128-017-2063-0

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  • DOI: https://doi.org/10.1007/s00128-017-2063-0

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