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Partial Gene Sequencing of CYP1A, Vitellogenin, and Metallothionein in Mosquitofish Gambusia yucatana and Gambusia sexradiata

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Abstract

Ground characteristics in the Yucatan Peninsula make recovery and treatment of wastewater very expensive. This situation has contributed to an increase of pollutants in the aquifer. Unfortunately, studies related to the effects of those pollutants in native organisms are scarce. The aim of this work was to obtain partial sequences of widely known genes used as biomarkers of pollutant effect in Gambusia yucatana and Gambusia sexradiata. The studied genes were: cytochrome P450 1A (CYP1A); vitellogenin (VTG); metallothionein (MT), and two housekeeping genes, 18S and β-actin. From reported sequences of Gambusia affinis, primers were designed and amplification was done in the local Gambusia species exposed for 48 h to gasoline (100 µL/L, stirred for 24 h pre-exposure). Preliminary results revealed partial sequences of all genes with an approximate average length of 200 bp. BLAST analysis of found sequences indicated a minimum of 97% identity with reported sequences for G. affinis or Gambusia holbrooki showing great similarity.

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Acknowledgements

The authors wish to acknowledge UNAM-PAPIIT-DGAPA grant IA200214 and the Postdoctoral Fellowship Program of the Mexican National Council of Science and Technology (CONACyT) for the financial support of this study. We also acknowledge Ismael Oceguera-Vargas, Anita Arroyo-Silva and Fernando Mex-Esquivel for their valuable efforts during the field work.

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Authors and Affiliations

  1. Unidad de Química en Sisal, Facultad de Química, Universidad Nacional Autónoma de México, Puerto de Abrigo s/n, 97356, Sisal, YUC, Mexico

    Roberto Vázquez-Euán & Gabriela Rodríguez-Fuentes

  2. Unidad Multidisciplinaria de Docencia e Investigación, Facultad de Ciencias, Universidad Nacional Autónoma de México, Puerto de Abrigo s/n, 97356, Sisal, YUC, Mexico

    Karla S. Escalante-Herrera

  3. CONACYT-Departamento de Investigaciones Científicas y Tecnológicas de la Universidad de Sonora, Hermosillo, SON, Mexico

    Roberto Vázquez-Euán

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  1. Roberto Vázquez-Euán
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  2. Karla S. Escalante-Herrera
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  3. Gabriela Rodríguez-Fuentes
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Correspondence to Gabriela Rodríguez-Fuentes.

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Vázquez-Euán, R., Escalante-Herrera, K.S. & Rodríguez-Fuentes, G. Partial Gene Sequencing of CYP1A, Vitellogenin, and Metallothionein in Mosquitofish Gambusia yucatana and Gambusia sexradiata . Bull Environ Contam Toxicol 98, 41–45 (2017). https://doi.org/10.1007/s00128-016-1994-1

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