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Extracellular Polymeric Substances of Aphanizomenon flos-aquae (EPS-A) Induced Apoptosis in Astrocytes of Zebrafish

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Abstract

In this study, extracellular polymeric substances of Aphanizomenon flos-aquae (EPS-A) were investigated in order to explore their effect on astrocytes of zebrafish and potential risk for environment. Astrocytes were treated with varying concentrations of EPS-A, the results showed that EPS-A inhibited astrocytes growth in a dose-and time-dependent manner. With the concentrations of EPS-A increasing, the adherent ability of astrocytes decreased and the number of astrocytes floating in the culture medium increased. When treated with 2.35 µg/mL EPS-A, EPS-A induced cell cycle arrest and made the collapse of mitochondrial membrane potential and then led to astrocytes apoptosis. The results suggested that EPS-A could pose a threat to zebrafish and represent risk for environment, so regularly monitoring the presence of EPS-A was very important in nutrient-rich freshwaters when A. flos-aquae blooms broke out.

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Acknowledgments

This work was supported by Program for Changjiang Scholars and Innovative Research Team in University (IRT0966) and by the National Natural Science Foundation of China (No. 31560234).

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Authors and Affiliations

  1. School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou, 730070, Gansu, China

    Ying Lv, Ling Tao & Jun Ren

  2. Department of Anesthesiology, Gansu Provincial Maternity and Child-Care Hospital, Lanzhou, 730050, Gansu, China

    Xing Xue

  3. Department of Life Science and Biotechnology, College of Science, Wuhan University of Technology, Wuhan, 430070, Hubei, China

    Delu Zhang

  4. Key Laboratory of Algal Biology, Institute of Hydrobiology, The Chinese Academy of Sciences, Wuhan, 430072, Hubei, China

    Chunxiang Hu

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  1. Ying Lv
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  2. Xing Xue
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  3. Ling Tao
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  5. Chunxiang Hu
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Correspondence to Jun Ren.

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Lv, Y., Xue, X., Tao, L. et al. Extracellular Polymeric Substances of Aphanizomenon flos-aquae (EPS-A) Induced Apoptosis in Astrocytes of Zebrafish. Bull Environ Contam Toxicol 96, 320–325 (2016). https://doi.org/10.1007/s00128-016-1729-3

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  • DOI: https://doi.org/10.1007/s00128-016-1729-3

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