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Tolerance and Antioxidant Response of a Dark Septate Endophyte (DSE), Exophiala pisciphila, to Cadmium Stress

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Abstract

The growth, oxidative damage and antioxidant response of Exophiala pisciphila ACCC32496, a dark septate endophyte isolated from an abandoned lead–zinc mining area, were measured at cadmium (Cd) concentrations of 0, 25, 50, 100, 200 and 400 mg L−1. The EC50 values of E. pisciphila ACCC32496 to Cd were 332.2 mg L−1 after 30 days on solid medium and 111.2 mg L−1 after 7 days in liquid medium. Cd stress markedly stimulated the production of superoxide anion, H2O2 and malondialdehyde in the fungal mycelia. The activities of superoxide dismutase and catalase reached their maxima at 100 mg L−1 Cd. The glutathione and non-protein thiol contents, along with the total antioxidant capability, reached their maxima at 50 mg L−1 Cd. Low Cd concentrations induced a noticeable increase in antioxidant defense, while high Cd concentrations decreased the antioxidant defense.

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References

  • Addy HD, Piercey MM, Currah RS (2005) Microfungal endophytes in roots. Botany 83:1–13

    Google Scholar 

  • Azevedo MM, Carvalho A, Pascoal C, Rodrigues F, Cassio F (2007) Responses of antioxidant defenses to Cu and Zn stress in two aquatic fungi. Sci Total Environ 377:233–243

    Article  CAS  Google Scholar 

  • Ban Y, Tang M, Chen H, Xu Z, Zhang H, Yang Y (2012) The response of dark septate endophytes (DSE) to heavy metals in pure culture. PLoS One 7(10):e47968

    Article  CAS  Google Scholar 

  • Deram A, Languereau-Leman F, Howsam M, Petit D, Haluwyn CV (2008) Seasonal patterns of cadmium accumulation in Arrhenatherum elatius (Poaceae): influence of mycorrhizal and endophytic fungal colonisation. Soil Biol Biochem 40:845–848

    Article  CAS  Google Scholar 

  • Diao YH, Li T, Zhao ZW (2013) Zinc accumulation characteristics of two Exophiala strains and their antioxidant response to Zn2+ stress. J Environ Prot 4:12–19

    Article  CAS  Google Scholar 

  • Elstner EF, Heupel A (1976) Inhibition of nitrite formation from hydroxylammoniumchloride: a simple assay for superoxide dismutase. Anal Biochem 70:616–620

    Article  CAS  Google Scholar 

  • Guelfi A, Azevedo RA, Lea PJ, Molina SMG (2003) Growth inhibition of the filamentous fungus Aspergillus nidulans by cadmium: an antioxidant enzyme approach. J Gen Appl Microbiol 49:63–73

    Article  CAS  Google Scholar 

  • Guimaraes-Soares L, Pascoal C, Cassio F (2007) Effects of heavy metals on the production of thiol compounds by the aquatic fungi Fontanospora fusiramosa and Flagellospora curta. Ecotoxicol Environ Saf 66:36–43

    Article  CAS  Google Scholar 

  • Hasan SA, Fariduddin Q, Ali B, Hayat S, Ahmad A (2009) Cadmium: toxicity and tolerance in plants. J Environ Biol 30:165–174

    CAS  Google Scholar 

  • Hegedus N, Emri T, Szilagyi J, Karanyi Z, Nagy I, Penninckx MJ, Pocsi I (2007) Effect of heavy metals on the glutathione status in different ectomycorrhizal Paxillus involutus strains. World J Microbiol Biotechnol 23:1339–1343

    Article  CAS  Google Scholar 

  • Jaeckel P, Krauss G, Krauss G (2005) Cadmium and zinc response of the fungi Heliscus lugdunensis and Verticillium cf. alboatrum isolated from highly polluted water. Sci Total Environ 346:274–279

    Article  CAS  Google Scholar 

  • Janero DR (1990) Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Radical Bio Med 9:515–540

    Article  CAS  Google Scholar 

  • Jumpponen A, Trappe JM (1998) Dark septate endophytes: a review of facultative biotrophic root-colonizing fungi. New Phytol 140:295–310

    Article  Google Scholar 

  • Li DD, Zhou DM, Wang P, Weng NY, Zhu XD (2011a) Subcellular Cd distribution and its correlation with antioxidant enzymatic activities in wheat (Triticum aestivum) roots. Ecotox Environ Saf 74:874–881

    Article  CAS  Google Scholar 

  • Li T, Liu MJ, Zhang XT, Zhang HB, Sha T, Zhao ZW (2011b) Improved tolerance of maize (Zea mays L.) to heavy metals by colonization of a dark septate endophyte (DSE) Exophiala pisciphila. Sci Total Environ 409:1069–1074

    Article  CAS  Google Scholar 

  • Li H, Li D, He C, Zhou Z, Mei T, Xu H (2012) Diversity and heavy metal tolerance of endophytic fungi from six dominant plant species in a Pb–Zn mine wasteland in China. Fungal Ecol 5:309–315

    Article  Google Scholar 

  • Miersch J, Neumann D, Menge S, Barlocher F, Baumbach R, Lichtenberger O (2005) Heavy metals and thiol pool in three strains of Tetracladium marchalianum. Mycol Prog 4:185–194

    Article  Google Scholar 

  • Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7:405–410

    Article  CAS  Google Scholar 

  • Mukherjee A, Das D, Kumar Mondal S, Biswas R, Kumar Das T, Boujedaini N, Khuda-Bukhsh AR (2010) Tolerance of arsenate-induced stress in Aspergillus niger, a possible candidate for bioremediation. Ecotoxicol Environ Saf 73:172–182

    Article  CAS  Google Scholar 

  • Paraszkiewicz K, Bernat P, Naliwajski M, Długonski J (2010) Lipid peroxidation in the fungus Curvularia lunata exposed to nickel. Arch Microbiol 192:135–141

    Article  CAS  Google Scholar 

  • Pocsi I, Prade RA, Penninckx MJ (2004) Glutathione, altruistic metabolite in fungi. Adv Microb Physiol 49:1–76

    Article  CAS  Google Scholar 

  • Raspanti E, Cacciola SO, Gotor C, Romero LC, Garcia I (2009) Implications of cysteine metabolism in the heavy metal response in Trichoderma harzianum and in three Fusarium species. Chemosphere 76:48–54

    Article  CAS  Google Scholar 

  • Razinger J, Dermastia M, Koce JD, Zrimec A (2008) Oxidative stress in duckweed (Lemna minor L.) caused by short-term cadmium exposure. Environ Pollut 153:687–694

    Article  CAS  Google Scholar 

  • Rehman A, Anjum MS (2011) Multiple metal tolerance and biosorption of cadmium by Candida tropicalis isolated from industrial effluents: glutathione as detoxifying agent. Environ Monit Assess 174:585–595

    Article  CAS  Google Scholar 

  • Serafin Munoz AH, Wrobel K, Gutierrez Corona JF, Wrobel K (2007) The protective effect of selenium inorganic forms against cadmium and silver toxicity in mycelia of Pleurotus ostreatus. Mycol Res 111:626–632

    Article  CAS  Google Scholar 

  • Wang SH, Zhang H, Cui YR, He QY, Zhang Q (2008) Effects of cadmium stress on the antioxidative system in Ganoderma lucidum mycelia. Chin J Appl Ecol 19:1355–1361

    CAS  Google Scholar 

  • Xu H, Song P, Gu W, Yang Z (2011) Effects of heavy metals on production of thiol compounds and antioxidant enzymes in Agaricus bisporus. Ecotoxicol Environ Saf 74:1685–1692

    Article  CAS  Google Scholar 

  • Zhan FD, He YM, Zu YQ, Li T, Zhao ZW (2011) Characterization of melanin isolated from a dark septate endophyte (DSE), Exophiala pisciphila. World J Microbiol Biotechnol 27:2483–2489

    Article  CAS  Google Scholar 

  • Zhang YJ, Zhang Y, Liu MJ, Shi XD, Zhao ZW (2008) Dark septate endophyte (DSE) fungi isolated from metal polluted soils: their taxonomic position, tolerance, and accumulation of heavy metals in vitro. J Microbiol 46:624–632

    Article  Google Scholar 

  • Zhang WW, Hu YJ, Cao YR, Huang FG, Xu H (2012) Tolerance of lead by the fruiting body of Oudemansiella radicata. Chemosphere 88:467–475

    Article  CAS  Google Scholar 

  • Zhang Y, Li T, Zhao ZW (2013) Colonization characteristics and composition of dark septate endophytes (DSE) in a lead and zinc slag heap in Southwest China. Soil Sediment Contam 22:532–545

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was financially supported by The National Natural Science Foundation of China (Nos. 41101486, 31160009, 31460114, U1202236).

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Authors and Affiliations

  1. College of Resources and Environment, Yunnan Agricultural University, Kunming, 650201, China

    Fangdong Zhan & Yongmei He

  2. Laboratory for Conservation and Utilization of Bio-Resources, and Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming, 650091, China

    Fangdong Zhan, Tao Li & Zhiwei Zhao

  3. Soil and Water Quality Laboratory, Gulf Coast Research and Education Center, University of Florida, Wimauma, FL, 33598, USA

    Yun-ya Yang & Gurpal S. Toor

Authors
  1. Fangdong Zhan
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  2. Yongmei He
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  3. Tao Li
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  4. Yun-ya Yang
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  5. Gurpal S. Toor
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  6. Zhiwei Zhao
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Correspondence to Zhiwei Zhao.

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Zhan, F., He, Y., Li, T. et al. Tolerance and Antioxidant Response of a Dark Septate Endophyte (DSE), Exophiala pisciphila, to Cadmium Stress. Bull Environ Contam Toxicol 94, 96–102 (2015). https://doi.org/10.1007/s00128-014-1401-8

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  • DOI: https://doi.org/10.1007/s00128-014-1401-8

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