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A Comparative Study of the Sensitivities of Salmonella typhimurium Strains TA 98, TA 100 and TA 102 to Hospital Waste Waters

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Abstract

Hospitals are a release source of many chemical compounds in their wastewaters. In the present study Salmonella typhimurium strains TA 98, TA 100 and TA 102 were analyzed for their sensitivity to hospital waste waters. The results of the study showed that hospital waste waters consists of mutagens causing frame shift mutations and base pair substitutions and amongst the three strains used in this study, TA 102 was most effective which along with TA 98 can be used for quick assessment of genotoxicity of hospital waste waters prior to its discharge. Genotoxic potential of hospital waste waters from five major hospitals located in Jaipur and Delhi was studied. Such waste waters should be treated prior to their discharge. The results of this study call for further detailed study in this area of research.

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References

  • DeFlora S, Comoirano A, Zanacchi P, Bennicelli C (1984) Mutagenicity testing with TA97 and TA102 of 30 DNA-damaging compounds, negative with other Salmonella strains. Mutat Res 134:159–165

    Article  CAS  Google Scholar 

  • Dunnett CW (1955) A multiple comparison procedure for comparing several treatments with a control. JM Stat Assoc 50:1096–1121

    Article  Google Scholar 

  • Emmanuel E, Blanchard JM, Keck G, Perrodin Y (2001) Chemiocal, biological and ecotoxicological characterisation of hospital effluents. De´chets Sciences etTechniques revue francophone d’ecologie industrielle 22(2):31–33

    Google Scholar 

  • Fan M, Byrd C, Compadre CM, Compadre RL (1998) Comparison of CoMFA models for Salmonella typhimurium TA98, TA100, TA98 S9 and TA100 S9 mutagenicity of nitroaromatics, SAR and QSAR. Environ Res 9(3):187–215

    CAS  Google Scholar 

  • Gatehouse D, Haworth S, Cebula T, Gocke YE, Kiev L, Matsushima T, Melcion C, Nokmi T, Ohta T, Venih S, Zeiger E (1994) Recommendations for the performance of bacterial mutation assays. Mutat Res 312:217–233

    Article  CAS  Google Scholar 

  • Gautam AK, Sunil K, Sabumon PC (2006) Preliminary study of physico-chemical treatment options for hospital wastewater. J Environ Manag 83:298–306

    Article  Google Scholar 

  • Gupta P, Bhatnagar P, Mathur N (2009) Genotoxcity evaluation of hospital waste water. Ecotoxicol Environ Saf 72(7):1925–1932

    Article  CAS  Google Scholar 

  • Hartman PE, Ames BN, Roth JR, Barnes WN, Levin DE (1986) Target sequences for mutagenesis in Salmonella histidine-requiring mutants. Environ Mutagen 8:631–641

    Article  CAS  Google Scholar 

  • Hartmann A, Golet EM, Gartiser S, Alder AC, Koller T, Widmer RM (1999) Primary DNA damage but not mutagenicity correlates with ciprofloxacin concentrations in German hospital wastewaters. Arch Environ Contam Toxicol 36:115–119

    Article  CAS  Google Scholar 

  • Jolibois B, Guerbet M (2005) Hospital wastewater genotoxicity. Ann Occup Hyg 50(2):89–196

    Article  Google Scholar 

  • Kaur K, Mathur N, Bhatnagar P (2012) Comparative study of usage of microbial strains for monitoring waste water treatment plants. Univ J Environ Res Technol 2(2):26–37

    CAS  Google Scholar 

  • Koivusalo M, Pukkala E, Vartiainen T (1997) Drinking water chlorination and cancer: a historical cohort study in Finland. Cancer Causes Control 8:192–200

    Article  CAS  Google Scholar 

  • Kümmerer K (2001) Drugs in the environment: emission of drugs, diagnostic aids and disinfectants into wastewater by hospitals in relation to other sources: a review. Chemosphere 45:957–969

    Article  Google Scholar 

  • Levin DE, Ames BN (1986) Classifying mutagens as to their specificity in causing the six possible transitions and transversions: a simple analysis using the Salmonella mutagenicity assay. Environ Mutagen 8:9–28

    Article  CAS  Google Scholar 

  • Levin DE, Hollstein M, Christman MF (1982) A new Salmonella tester strain (TA 102) with A X T base pairs at the site of mutation detects oxidative mutagens. Proc Natl Acad Sci USA 79:7445–7449

    Article  CAS  Google Scholar 

  • Mahon GAT, Middleton B, Robinson WD, Green MHL, IdeG M, Tweats DJ (1989) Analysis of data from microbial colony assays. In: Kirkland DJ (ed) Statistical evaluation of mutagenicity test data. UKEMS. Sub-committee on Guidelines for Mutagenicity Testing Cambridge University Press, Cambridge, pp 26–65 Part III

    Google Scholar 

  • Maron DM, Ames BN (1983) Revised methods for the Salmonella mutagenicity test. Mutat Res 133:173–215

    Article  Google Scholar 

  • Mathur N, Bhatnagar P, Bakre P (2005) Assessing mutagenicity of textile dyes from Pali (Rajasthan) using Ames bioassay. Appl Ecol Environ Res 4(1):111–118

    Article  Google Scholar 

  • Mathur N, Bhatnagar P, Mohan K, Bakre P, Nagar P, Bijarnia MK (2006) Mutagenicity evaluation of industrial sludge from common effluent treatment plant. Chemosphere 67:1229–1235

    Article  Google Scholar 

  • Mortelmans K, Zeiger E (2000) The Ames Salmonella/microsome mutagenicity assay. Mutat Res 455:29–60

    Article  CAS  Google Scholar 

  • Prival MJ, Bell SJ, Mitchell VD, Vaughan VL (1982) Mutagenicity of benzidine and benzidine congener dyes and selected monoazo dyes in a modified Salmonella assay. Mutat Res 136:33–47

    Article  Google Scholar 

  • Vincent-Hubert F, Heas-Moisan K, Munschy C, Tronczynski J (2012) Mutagenicity and genotoxicity of suspended particulate matter in the Seine river estuary. Mutat Res 741(1–2):7–12

    Article  CAS  Google Scholar 

  • White PA, Rasmussen JB (1998) The genotoxic hazards of domestic wastes in surface waters. Mutat Res 410:223–236

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors are thankful to University Grants Commission’s for providing financial support under Dr. DS Kothari’s post doctoral fellowship scheme F.No. F.4-2/2006 (BSR)/13-424/2011 (BSR).

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Correspondence to Preeti Gupta.

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Gupta, P., Mathur, P., Mathur, N. et al. A Comparative Study of the Sensitivities of Salmonella typhimurium Strains TA 98, TA 100 and TA 102 to Hospital Waste Waters. Bull Environ Contam Toxicol 93, 95–100 (2014). https://doi.org/10.1007/s00128-014-1274-x

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  • DOI: https://doi.org/10.1007/s00128-014-1274-x

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