Abstract
Hospitals are a release source of many chemical compounds in their wastewaters. In the present study Salmonella typhimurium strains TA 98, TA 100 and TA 102 were analyzed for their sensitivity to hospital waste waters. The results of the study showed that hospital waste waters consists of mutagens causing frame shift mutations and base pair substitutions and amongst the three strains used in this study, TA 102 was most effective which along with TA 98 can be used for quick assessment of genotoxicity of hospital waste waters prior to its discharge. Genotoxic potential of hospital waste waters from five major hospitals located in Jaipur and Delhi was studied. Such waste waters should be treated prior to their discharge. The results of this study call for further detailed study in this area of research.
Similar content being viewed by others
References
DeFlora S, Comoirano A, Zanacchi P, Bennicelli C (1984) Mutagenicity testing with TA97 and TA102 of 30 DNA-damaging compounds, negative with other Salmonella strains. Mutat Res 134:159–165
Dunnett CW (1955) A multiple comparison procedure for comparing several treatments with a control. JM Stat Assoc 50:1096–1121
Emmanuel E, Blanchard JM, Keck G, Perrodin Y (2001) Chemiocal, biological and ecotoxicological characterisation of hospital effluents. De´chets Sciences etTechniques revue francophone d’ecologie industrielle 22(2):31–33
Fan M, Byrd C, Compadre CM, Compadre RL (1998) Comparison of CoMFA models for Salmonella typhimurium TA98, TA100, TA98 S9 and TA100 S9 mutagenicity of nitroaromatics, SAR and QSAR. Environ Res 9(3):187–215
Gatehouse D, Haworth S, Cebula T, Gocke YE, Kiev L, Matsushima T, Melcion C, Nokmi T, Ohta T, Venih S, Zeiger E (1994) Recommendations for the performance of bacterial mutation assays. Mutat Res 312:217–233
Gautam AK, Sunil K, Sabumon PC (2006) Preliminary study of physico-chemical treatment options for hospital wastewater. J Environ Manag 83:298–306
Gupta P, Bhatnagar P, Mathur N (2009) Genotoxcity evaluation of hospital waste water. Ecotoxicol Environ Saf 72(7):1925–1932
Hartman PE, Ames BN, Roth JR, Barnes WN, Levin DE (1986) Target sequences for mutagenesis in Salmonella histidine-requiring mutants. Environ Mutagen 8:631–641
Hartmann A, Golet EM, Gartiser S, Alder AC, Koller T, Widmer RM (1999) Primary DNA damage but not mutagenicity correlates with ciprofloxacin concentrations in German hospital wastewaters. Arch Environ Contam Toxicol 36:115–119
Jolibois B, Guerbet M (2005) Hospital wastewater genotoxicity. Ann Occup Hyg 50(2):89–196
Kaur K, Mathur N, Bhatnagar P (2012) Comparative study of usage of microbial strains for monitoring waste water treatment plants. Univ J Environ Res Technol 2(2):26–37
Koivusalo M, Pukkala E, Vartiainen T (1997) Drinking water chlorination and cancer: a historical cohort study in Finland. Cancer Causes Control 8:192–200
Kümmerer K (2001) Drugs in the environment: emission of drugs, diagnostic aids and disinfectants into wastewater by hospitals in relation to other sources: a review. Chemosphere 45:957–969
Levin DE, Ames BN (1986) Classifying mutagens as to their specificity in causing the six possible transitions and transversions: a simple analysis using the Salmonella mutagenicity assay. Environ Mutagen 8:9–28
Levin DE, Hollstein M, Christman MF (1982) A new Salmonella tester strain (TA 102) with A X T base pairs at the site of mutation detects oxidative mutagens. Proc Natl Acad Sci USA 79:7445–7449
Mahon GAT, Middleton B, Robinson WD, Green MHL, IdeG M, Tweats DJ (1989) Analysis of data from microbial colony assays. In: Kirkland DJ (ed) Statistical evaluation of mutagenicity test data. UKEMS. Sub-committee on Guidelines for Mutagenicity Testing Cambridge University Press, Cambridge, pp 26–65 Part III
Maron DM, Ames BN (1983) Revised methods for the Salmonella mutagenicity test. Mutat Res 133:173–215
Mathur N, Bhatnagar P, Bakre P (2005) Assessing mutagenicity of textile dyes from Pali (Rajasthan) using Ames bioassay. Appl Ecol Environ Res 4(1):111–118
Mathur N, Bhatnagar P, Mohan K, Bakre P, Nagar P, Bijarnia MK (2006) Mutagenicity evaluation of industrial sludge from common effluent treatment plant. Chemosphere 67:1229–1235
Mortelmans K, Zeiger E (2000) The Ames Salmonella/microsome mutagenicity assay. Mutat Res 455:29–60
Prival MJ, Bell SJ, Mitchell VD, Vaughan VL (1982) Mutagenicity of benzidine and benzidine congener dyes and selected monoazo dyes in a modified Salmonella assay. Mutat Res 136:33–47
Vincent-Hubert F, Heas-Moisan K, Munschy C, Tronczynski J (2012) Mutagenicity and genotoxicity of suspended particulate matter in the Seine river estuary. Mutat Res 741(1–2):7–12
White PA, Rasmussen JB (1998) The genotoxic hazards of domestic wastes in surface waters. Mutat Res 410:223–236
Acknowledgments
The authors are thankful to University Grants Commission’s for providing financial support under Dr. DS Kothari’s post doctoral fellowship scheme F.No. F.4-2/2006 (BSR)/13-424/2011 (BSR).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Gupta, P., Mathur, P., Mathur, N. et al. A Comparative Study of the Sensitivities of Salmonella typhimurium Strains TA 98, TA 100 and TA 102 to Hospital Waste Waters. Bull Environ Contam Toxicol 93, 95–100 (2014). https://doi.org/10.1007/s00128-014-1274-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00128-014-1274-x