Skip to main content
Log in

Effect of 1-Octyl-3-methylimidazolium Chloride on Cell Replication and Membrane Permeability of Escherichia coli DH5α

  • Published:
Bulletin of Environmental Contamination and Toxicology Aims and scope Submit manuscript

Abstract

Ionic liquids (ILs) have elicited attention due to their unique properties. ILs may pose environmental risks to aquatic ecosystems once released into water during generation and application. Therefore, the toxic and antimicrobial properties of ILs should be analysed. This study aims to evaluate the cytotoxicity of 1-octyl-3-methylimidazolium chloride ([C8mim] [Cl]) on Escherichia coli DH5α by MTT (3-[4,5-dimethylthiazol-2yl]-2,5 diphenyl tetrazolium bromide) assay, and to determine the effect of [C8mim] [Cl] on the replication and membrane permeability of E. coli DH5α. The results showed that [C8mim] [Cl] decreased cell viability and inhibited bacterial cell replication. [C8mim] [Cl] increased protein and nucleic acid contents in the extracellular fluid, damaged the cell membrane, and increased membrane permeability. The increase of cell membrane permeability induced by [C8mim] [Cl] could be the cause of decreased cell viability and replication.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Brennecke JF, Maginn EJ (2001) Ionic liquids: innovative fluids for chemical processing. AIChE J 47:2384–2389

    Article  CAS  Google Scholar 

  • Clark JH (1991) Green chemistry: challenges and opportunities. Green Chem 1:1–8

    Article  Google Scholar 

  • Couling DJ, Bernot RJ, Docherty KM, Dixon JK, Maginn EJ (2006) Assessing the factors responsible for ionic liquid toxicity to aquatic organisms via quantitative structure–property relationship modeling. Green Chem 8:82–90

    Article  CAS  Google Scholar 

  • Czerwicka M, Stolte S, Muller A, Siedlecka EM, Golebiowski M, Kumirska J, Stepnowski P (2009) Identification of ionic liquid breakdown products in an advanced oxidation system. J Hazard Mater 171:478–483

    Article  CAS  Google Scholar 

  • Dipeolu O, Green E, Stephens G (2008) Effects of water-miscible ionic liquids on cell growth and nitro reduction using Clostridium sporogenes. Green Chem 11:397–401

    Article  Google Scholar 

  • Garćıa-Lorenzo A, Tojo E, Tojo J, Teijeira M, Rodŕıguez-Berrocal FJ, Gonźalez MP, Marínez-Zorzano VS (2008) Cytotoxicity of selected imidazolium-derived ionic liquids in the human Caco-2 cell line. Sub-structural toxicological interpretation through a QSAR study. Green Chem 10:508–516

    Article  Google Scholar 

  • Gathergood N, Garcia MT, Scammells PJ (2004) Biodegradable ionic liquids: part I. Concept, preliminary targets and evaluation. Green Chem 6:166–175

    Article  CAS  Google Scholar 

  • Jing C, Li X, Zhang J, Wang J (2013a) Responses of the antioxidant system in QGY-7701 cells to the cytotoxicity and apoptosis induced by 1-octyl-3-methylimidazolium chloride. J Biochem Mol Toxicol 27:330–336

    Article  CAS  Google Scholar 

  • Jing CQ, Chen HL, Li XY (2013b) Cytotoxicity and possible mechanism of 1-octyl-3-methylimidazolium chloride on EMT6 cells. Acta Scientiae Circumstantiae 33:1809–1814 (in Chinese)

    CAS  Google Scholar 

  • Latała A, Nędzi M, Stepnowski P (2010) Toxicity of imidazolium ionic liquids towards algae. Influence of salinity variations. Green Chem 12:60–64

    Article  Google Scholar 

  • Li XY, Zeng SH, Dong XY, Ma JG, Wang JJ (2011) Acute toxicity and responses of antioxidant systems to 1-methyl-3-octylimidazolium bromide at different developmental stages of goldfish. Ecotoxicology 21:253–259

    Article  Google Scholar 

  • Li XY, Jing CQ, Zang XY, Yang S, Wang JJ (2012a) Toxic cytological alteration and mitochondrial dysfunction in PC12 cells induced by 1-octyl-3-methylimidazolium chloride. Toxicol In Vitro 26:1087–1092

    Article  CAS  Google Scholar 

  • Li XY, Zeng SH, Dong XY, Ma JG, Wang JJ (2012b) Acute toxicity and responses of antioxidant systems to 1-methyl-3-octylimidazolium bromide at different developmental stages of goldfish. Ecotoxicology 21:253–259

    Article  CAS  Google Scholar 

  • Luo YR, Li XY, Chen XX, Zhang BJ, Sun ZJ, Wang JJ (2008) The developmental toxicity of 1-methyl-3-octylimidazolium bromide on Daphnia magna. Environ Toxicol 23:736–744

    Article  CAS  Google Scholar 

  • Ma JM, Cai LL, Zhang BJ, Hu LW, Li XY, Wang JJ (2010) Acute toxicity and effects of 1-alkyl-3-methylimidazolium bromide ionic liquids on green algae. Ecotoxicol Environ Saf 73:1465–1469

    Article  CAS  Google Scholar 

  • Petkovic M, Ferguson JL, Nimal Gunaratne HQ, Ferreira R, Leitão MC, Seddon KR, Rebelo LPN, Pereira CS (2010) Novel biocompatible cholinium-based ionic liquids: toxicity and biodegradability. Green Chem 12:643–649

    Article  CAS  Google Scholar 

  • Pham TP, Cho CW, Yun YS (2010) Environmental fate and toxicity of ionic liquids: a review. Water Res 44:352–372

    Article  CAS  Google Scholar 

  • Ranke J, Molter K, Stock F, Bottin-Weber U, Poczobutt J, Hoffmann J, Ondruschka B, Filser J, Jastorff B (2004) Biological effects of imidazolium ionic liquids with varying chain lengths in acute Vibrio fischeri and WST-1 cell viability assays. Ecotoxicol Environ Saf 58:396–404

    Article  CAS  Google Scholar 

  • Ranke J, Muller A, Bottin-Weber U, Stock F, Stolte S, Arning J, Stormann R, Jastorff B (2007a) Lipophilicity parameters for ionic liquid cations and their correlation to in vitro cytotoxicity. Ecotoxicol Environ Saf 67:430–438

    Article  CAS  Google Scholar 

  • Ranke J, Stolte S, Störmann R, Arning J, Jastorff B (2007b) Design of sustainable chemical products: the example of ionic liquids. Chem Rev 107:2183–2206

    Article  CAS  Google Scholar 

  • Samori C, Malferrari D, Valbonesi P, Montecavalli A, Moretti F, Galletti P, Sartor G, Tagliavini E, Fabbri E, Pasteris A (2010) Introduction of oxygenated side chain into imidazolium ionic liquids: evaluation of the effects at different biological organization levels. Ecotoxicol Environ Saf 73:1456–1464

    Article  CAS  Google Scholar 

  • Sheldon R (2001) Catalytic reactions in ionic liquids. Chem Commun 23:2399–2407

    Article  Google Scholar 

  • Ventura SP, Goncalves AM, Goncalves F, Coutinho JA (2010) Assessing the toxicity on [C3mim][Tf2 N] to aquatic organisms of different trophic levels. Aquatic Toxicol 96:290–297

    Article  CAS  Google Scholar 

  • Ventura SP, Marques CS, Rosatella AA, Afonso CA, Goncalves F, Coutinho JA (2012) Toxicity assessment of various ionic liquid families towards Vibrio fischeri marine bacteria. Ecotoxicol Environ Saf 76:162–168

    Article  CAS  Google Scholar 

  • Welton T (1999) Room-temperature ionic liquids. Solvents for synthesis and catalysis. Chem Rev 99:2071–2084

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This research was supported by the Science and Technology Foundation of Henan Province, China (No: 12A180022).

Conflict of interest

The authors report no conflict of interest.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Changqin Jing.

Additional information

Lingmin Mu have contributed equally to the first author.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jing, C., Mu, L., Ren, T. et al. Effect of 1-Octyl-3-methylimidazolium Chloride on Cell Replication and Membrane Permeability of Escherichia coli DH5α. Bull Environ Contam Toxicol 93, 60–63 (2014). https://doi.org/10.1007/s00128-014-1269-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00128-014-1269-7

Keywords

Navigation