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Effects of Glyphosate on Growth Rate, Metabolic Rate and Energy Reserves of Early Juvenile Crayfish, Cherax quadricarinatus M.

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Abstract

Early juveniles of the crayfish Cherax quadricarinatus were exposed for 60 days to 10 and 40 mg/L of pure glyphosate (acid form) in freshwater. Mortality was 33 % at the highest concentration, while no differences in molting were noted among treatments. After the first month of exposure, weight gain was significantly (p < 0.05) reduced in the 40 mg/L group. At the end of the assay, lipid levels in muscle, as well as protein level in both hepatopancreas and muscle were significantly (p < 0.05) reduced. These results suggest long-term utilization of both lipid and protein as main energetic reserves, likely in response to the chronic stress associated with herbicide exposure. Besides, the lower pyruvate kinase activity in muscle suggests a possible metabolic depression in this tissue. The hemolymphatic ASAT:ALAT ratio showed higher levels than the control at the highest glyphosate concentration, indicating possible damage to several tissues.

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Acknowledgments

This work was supported by Grants from ANPCYT (PICT 2010-0908), UBACYT 2012-2015 (code 044) and 2011–2014 scientific programs, UBACYT 2011–2014 (code 01/W985), and PIP CONICET (2010–2012 program, code 100884). We also thank Dr. Itzick Vatnick for style revision.

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Authors and Affiliations

  1. Department of Biodiversity and Experimental Biology-IBBEA-CONICET, University of Buenos Aires, Ciudad Universitaria, Pab. II, C1428EHA, Buenos Aires, Argentina

    L. Avigliano, D. A. Medesani & E. M. Rodríguez

  2. Department of Biological Chemistry, IQUIBICEN-CONICET. FCEyN, University of Buenos Aires, Ciudad Universitaria, Pab. II, C1428EHA, Buenos Aires, Argentina

    A. V. Fassiano & M. C. Ríos de Molina

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  1. L. Avigliano
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  2. A. V. Fassiano
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  3. D. A. Medesani
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  4. M. C. Ríos de Molina
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  5. E. M. Rodríguez
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Correspondence to E. M. Rodríguez.

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Avigliano, L., Fassiano, A.V., Medesani, D.A. et al. Effects of Glyphosate on Growth Rate, Metabolic Rate and Energy Reserves of Early Juvenile Crayfish, Cherax quadricarinatus M.. Bull Environ Contam Toxicol 92, 631–635 (2014). https://doi.org/10.1007/s00128-014-1240-7

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  • DOI: https://doi.org/10.1007/s00128-014-1240-7

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