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In Situ Assessment of the Paraguay River Water, in Brazilian Pantanal, by Means of Micronucleus Assay with Fish and Chemical Analysis

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Abstract

The aim of this research was to assess water quality in a stretch of the Paraguay River within the Brazilian Pantanal by means of a micronucleus assay with fish, and by water and sediment physicochemical analysis. Significant increases (p >0.05) in the frequency of micronuclei (MN) and micronucleated cells (MNC) occurred in erythrocytes of Pimelodus maculatus and Leporinus friderici at two river sites in the town of Caceres relative to an upstream reference site. The results demonstrate that the Paraguay River water near Caceres has been receiving genotoxic effluents, which may be associated with the presence of chromium, sulfides, oil and grease, and/or other chemicals.

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References

  • Abdo MSA, Da Silva CJ (2004) Limnological characteristics of the water bodies of the Corutuba nesting site in Brazil’s Pantanal. Acta Limnol Bras 16(4):359–368

    Google Scholar 

  • Al-Sabti K, Metcalfe CD (1995) Fish micronuclei for assessing genotoxicity in water. Mutat Res 343:121–135

    Article  CAS  Google Scholar 

  • Assine ML, Soares PC (2004) Quaternary of the pantanal, west-central Brazil. Quatern Int 114:23–34

    Article  Google Scholar 

  • Ayllón F, Suciu R, Gephard S, Juanes F, Garciá-Vázquez E (2000) Conventional armament wastes induce micronuclei in wild brown trout Salmo trutta. Mutat Res 470:169–176

    Article  Google Scholar 

  • Bresler V, Bissinger V, Abelson A, Dizer H, Sturm A, Kratke R, Fishelson L, Hansen PD (1999) Marine molluscs and fish as biomarkers of pollution stress in littoral regions of the red sea, mediterranean sea and north sea. Helgoland Mar Res 53:219–243

    Article  Google Scholar 

  • Buss DF, Baptista DF, Nessimian JL (2003) Conceptual basis for the application of biomonitoring on stream water quality programs. Caderno Saúde Pública, Rio de Janeiro, Brazil, pp 465–473

  • Çavas T, Ergene-Gözükara S (2005a) Micronucleus test in fish cells: a bioassay for in situ monitoring of genotoxic pollution in the marine Environment. Environ Mol Mutagen 46:64–70

    Article  Google Scholar 

  • Çavas T, Ergene-Gözükara S (2005b) Induction of micronuclei and nuclear abnormalities in Oreochromis niloticus following exposure to petroleum refinery and chromium processing plant effluents. Aquat Toxicol 74(3):264–271

    Article  Google Scholar 

  • Çavas T, Könen S (2008) In vivo genotoxicity testing of the amnesic shellfish poison (domoic acid) in piscine erythrocytes using the micronucleus test and the comet assay. Aquat Toxicol 90:154–159

    Article  Google Scholar 

  • CONAMA - Resolução No 357 de 17/03/2005 do Conselho Nacional de meio Ambiente. (2005) MMA Brasil

  • Costa C, Silvari V, Melchinib A, Cataniab S, Heffronc JJ, Trovatob A, Pasqualeb RD (2009) Genotoxicity of imidacloprid in relation to metabolic activation and composition of the commercial product. Mutat Res 672:40–44

    Article  CAS  Google Scholar 

  • Cristaldi M, Ieradi LA, Udroiu I, Zilli R (2004) Comparative evaluation of background micronucleus frequencies in domestic mammals. Mutat Res 559:1–9

    Article  CAS  Google Scholar 

  • Dorea JG, Antonio C, Barbosa AC, Silva GS (2006) Fish mercury bioaccumulation as a function of feeding behavior and hydrological cycles of the Rio Negro, Amazon. Comp Biochem Physiol C Toxicol Pharmacol 142(3–4):275–283

    Article  Google Scholar 

  • Duez P, Dehon G, Kumps A, Dubois J (2003) Statistics of the Comet assay: a key to discriminate between genotoxic effects. Mutagenesis 18:159–166

    Article  CAS  Google Scholar 

  • FEMA (2003) Projeto Implementação de Práticas de Gerenciamento Integrado De Bacia Hidrográfica Para O Pantanal E Bacia Do Alto Paraguai. Available at: www.ana.gov.br/gefap—Componente III, Accessed 16 Jan 2012

  • Graf U, Heo OS, Ramirez OO (1992) The genotoxicity of chromium(VI) oxide in the wing spot test of Drosophila melanogaster is over 90% due to mitotic recombination. Mutat Res 266(2):197–203

    Article  CAS  Google Scholar 

  • Grisolia CK, Cordeiro CMT (2000) Variability in micronucleus induction with different mutagens applied to several species of fish. Genet Mol Biol 23(1):235–239

    Article  CAS  Google Scholar 

  • Grisolia CK, Starling FLR (2001) Micronuclei monitoring of fish from Lake Paranoá, under influence of sewage treatment plant discharges. Mutat Res 491:39–44

    Article  CAS  Google Scholar 

  • Grisolia CK, Oliveira ABBO, Bonfim H, Klautau-Guimarães MN (2005) Genotoxicity evaluation of domestic sewage in a municipal wastewater treatment plant. Genet Mol Biol 28(2):334–338

    Article  Google Scholar 

  • Hamilton SK, Sippel SJ, Calheiros DF, Melak JM (1997) An anoxic event and other biogeochemical effects of the Pantanal wetland on the Paraguay River. Limnol Oceanogr 42(2):257–272

    Article  CAS  Google Scholar 

  • Hayashi M, Ueda T, Uyeno K, Wada K, Kinae N, Saotome K, Tanaka N, Takai A, Sasaki YF, Asano N, Sofuni T, Ojiva Y (1998) Development of genotoxicity assay systems that use aquatic organisms. Mut Res 399:125–133

    Article  CAS  Google Scholar 

  • Hylander LD, Pinto FN, Guimarães JRD, Meili M, Oliveira LJ, Silva EC (2000) Fish mercury concentration in the Alto Pantanal, Brazil: influence of season and water parameters. Sci Total Environ 261:9–20

    Article  CAS  Google Scholar 

  • Lemos CT, Iranço FA, Oliveira NCD, Souza GD, Fachel JMG (2008) Biomonitoring of genotoxicity using micronuclei assay in native population of Astyanax jacuhiensis (Characiformes: characidae) at sites under petrochemical influence. Sci Total Environ 406:337–343

    Article  Google Scholar 

  • Liu JR, Don HW, Tang XL, Sun XR, Han XH, Chen BQ, Sun CH, Yang FB (2009) Genotoxicity of water from the Songhua River, China, in 1994–1995 and 2002–2003: potential risks for human health. Environ Pollut 157:357–364

    Article  CAS  Google Scholar 

  • Minissi S, Ciccotti E, Rizzoni M (1996) Micronucleus test in erythrocytes of Barbus plebejus (Teleostei, Pisces) from two natural environments: a bioassay for the in situ detection of mutagens in freshwater. Mutat Res 367:245–251

    Article  CAS  Google Scholar 

  • Nepomuceno JC, Ferrari I, Spanó MA, Centeno AJ (1997) Detection of micronuclei in peripheral erythrocytes of Cyprinus carpio exposed to metallic mercury. Environ Mol Mutagen 30:293–297

    Article  CAS  Google Scholar 

  • Organization of American States (2005) Pantanal and the upper Paraguay River. Available at: http://www.oas.org/dsd/Events/english/Documents/OSDE_3Pantanal.pdf Accessed 10 April 2012

  • Palhares D, Grisolia CG (2002) Comparison between the micronucleus frequencies of kidney and gill erythrocytes in tilapia fish, following mitomycin C treatment. Genet Mol Biol 25(3):282–284

    Article  Google Scholar 

  • Pimenta VMSD, Nepomuceno JC, Pavanin LA (2008) Genotoxicity of water from the paraguay river near caceres-MT, Brazil in the drosophila wing-spot Test. Environ Mol Mutagen 49(6):458–466

    Article  CAS  Google Scholar 

  • Prá D, Lau AH, Knakievicz T, Carneiro FR, Erdtmann B (2005) Environmental genotoxicity assessment of an urban stream using freshwater planarians. Mutat Res 585(1–2):79–85

    Google Scholar 

  • Rodrigues AL, Bellinaso ML, Dick T (1989) Effect of some metal ions on blood and liver delta-aminolevulinate dehydratase of Pimelodus maculatus (Pisces pimelodidae). Comp Biochem Phys B 94(1):65–69

    CAS  Google Scholar 

  • Sakuragui MM, Paulino MG, Henrique HS, Fernandes MN (2007) Na+/K+ATPase activity in the fish gills of Pimelodus maculatus. In: 7th international congress of comparative physiology and biochemistry, 2007, Salvador, BA, Brazil. Comp Biochem Phys A 148:S73

  • Schmid W (1975) Micronucleus test for cytogenetic analysis in: Hollanender A. chemical mutagens. principles and methods for their detection. plenum Press, New York and London. 4(36):31–53

  • Yi H, Meng Z (2003) Genotoxicity of hydrated sulfur dioxide on root tips of Allium sativum and Vicia faba. Mutat Res 537:109–114

    Article  CAS  Google Scholar 

  • Zar JH (2010) Biostatistical analysis. Prentice Hall, New Jersey, p 944

    Google Scholar 

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Acknowledgments

To the UNEMAT, UFU and the CAPES for the financial support. To Dr. Francisco Langeani, UNESP—São José do Rio Preto Campus, for identifying the species Leporinus friderici.

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Correspondence to Joaquim Manoel da Silva.

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Pimenta, V.M.S.D., da Silva, J.M., Nepomuceno, J.C. et al. In Situ Assessment of the Paraguay River Water, in Brazilian Pantanal, by Means of Micronucleus Assay with Fish and Chemical Analysis. Bull Environ Contam Toxicol 90, 427–433 (2013). https://doi.org/10.1007/s00128-012-0935-x

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  • DOI: https://doi.org/10.1007/s00128-012-0935-x

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