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Real-Time PCR Investigation of the Dynamic Expression of Three “RNA Processing and Modification” Genes of Phanerochaete chrysosporium Exposed to Lead

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Abstract

The expression of three ribosome binding proteins namely; polyadenylate-binding protein, splicing factor RNPS1 and ATP-dependent RNA helicase of Phanerochaete chrysosporium exposed to lead were analyzed by real-time PCR. The mRNA level of splicing factor RNPS1 showed 2.7 (p < 0.05), 2.6 (p < 0.05) and 4.9-fold (p < 0.001) increase when the cells were exposed to 25, 50 and 100 μM lead, respectively. 50 and 100 μM lead exposure resulted in almost 2-fold (p < 0.01and p < 0.05, respectively) increase in the expression of ATP-dependent RNA helicase. Polyadenylate-binding protein mRNA levels did not reveal any significant increase when cells exposed to the concentrations of lead employed. However, the mRNA level of polyadenylate-binding protein and splicing factor RNPS1 within a period of 1 and 2 h temporal exposure to 100 μM lead showed 2.5 (p < 0.001) and 3.4-fold (p < 0.001) increase, respectively. Expression level of ATP-dependent RNA helicase was not affected from the period of exposure to this metal.

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Acknowledgments

This study was supported by Middle East Technical University Research Fund METU BAP-07.02.2009.00.01.

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Authors and Affiliations

  1. Department of Biological Sciences, Middle East Technical University, 06800, Ankara, Turkey

    Elif Tekin & Gulay Ozcengiz

  2. Department of Environmental Engineering, Middle East Technical University, 06531, Ankara, Turkey

    Bulent Icgen

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  1. Elif Tekin
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  2. Bulent Icgen
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  3. Gulay Ozcengiz
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Correspondence to Bulent Icgen.

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Tekin, E., Icgen, B. & Ozcengiz, G. Real-Time PCR Investigation of the Dynamic Expression of Three “RNA Processing and Modification” Genes of Phanerochaete chrysosporium Exposed to Lead. Bull Environ Contam Toxicol 90, 27–33 (2013). https://doi.org/10.1007/s00128-012-0886-2

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  • DOI: https://doi.org/10.1007/s00128-012-0886-2

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