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Effects of Artemisinin on Photosystem II Performance of Microcystis aeruginosa by In Vivo Chlorophyll Fluorescence

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Abstract

Effects of artemisinin (derived from Artemisia annua) on the photosynthetic activity of Microcystis aeruginosa was investigated by using chlorophyll a (Chl a) fluorescence transient O-J-I–P and JIP-test after exposure to elevated artemisinin concentration. High artemisinin concentration resulted in a significant suppression in photosynthesis and respiration. Results showed that the OJIP curves flattened and the maximal fluorescence yield reached at the J step under artemisinin stress. The decreased values of the energy needed for the RCs’ closure (Sm) and the number of oxidation and reduction (N) suggested that the reduction times of primary bound plastoquinone (QA) was also decreased. The absorption flux (ABS/RC) per photosystem II (PSII) reaction center and the electron transport flux (ET0/RC) decreased with increasing artemisinin concentration. Excess artemisinin had little effect on the trapping flux (TR0/RC). The results showed that the decrease of photosynthesis in exposure to excess artemisinin may be a result of the inactivation of PSII reaction centers and the inhibition of electron transport in the acceptor side.

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Acknowledgments

This work has been supported jointly by the National Natural Science Foundation (Grant No.51109061); the Open Fund of the State Key Laboratory of Hydrology Water Resources and Hydraulic Engineering (Grant No. 2010490211) and the Outstanding Innovative Talents Project of Hohai University.

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Authors and Affiliations

  1. Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, MOE, School of Environment, Hohai University, Nanjing, 210098, China

    Lixiao Ni, Yong Li & Yiping Li

  2. Division of Hydrologic Sciences, Desert Research Institute, Las Vegas, NV, 89119, USA

    Lixiao Ni & Kumud Acharya

  3. Shanxi Lu’an Engineering Investigation and Design Co. Ltd., Changzhi, 046204, China

    Xiangyang Hao

  4. Department of Environmental Science and Engineering, School of Geography Science, Nanjing Normal University, Nanjing, 210097, China

    Shiyin Li

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  1. Lixiao Ni
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  2. Kumud Acharya
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  3. Xiangyang Hao
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  4. Shiyin Li
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  5. Yong Li
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Correspondence to Lixiao Ni.

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Ni, L., Acharya, K., Hao, X. et al. Effects of Artemisinin on Photosystem II Performance of Microcystis aeruginosa by In Vivo Chlorophyll Fluorescence. Bull Environ Contam Toxicol 89, 1165–1169 (2012). https://doi.org/10.1007/s00128-012-0843-0

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  • DOI: https://doi.org/10.1007/s00128-012-0843-0

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