Bulletin of Environmental Contamination and Toxicology

, Volume 82, Issue 6, pp 743-748

First online:

Evaluation of a Methodology for Toxicity Testing of Volatile Chlorinated Hydrocarbons on Marine Organisms

  • James HuntAffiliated withEnvironmental Geology Group, School of Geosciences, University of Sydney Email author 
  • , Gavin BirchAffiliated withEnvironmental Geology Group, School of Geosciences, University of Sydney
  • , Michael St. J. WarneAffiliated withCentre for Environmental Contaminants Research, CSIRO
  • , Rick KrassoiAffiliated withEcotox Services Australasia Pty Ltd

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This study evaluated the suitability of sealed containers for toxicity testing to prevent loss of volatile chlorinated hydrocarbons (VCHs) with a range of Australian marine organisms including: micro-algae; sea urchin and oyster larvae in 44 mL sealed vials and fish larvae; amphipods; and juvenile polychaetes in 1 L sealed jars. Vials prevented volatilisation of VCHs during testing. Jars were less effective, with average losses of 46%. Growth and development of algae, sea urchins and oysters in vials was acceptable, indicating suitability of the methodology. Jars were suitable for amphipods and polychaetes; however, further evaluation of the fish test is required.


Volatile chlorinated hydrocarbons Toxicity testing Sealed containers