Bulletin of Environmental Contamination and Toxicology

, Volume 82, Issue 6, pp 743–748

Evaluation of a Methodology for Toxicity Testing of Volatile Chlorinated Hydrocarbons on Marine Organisms


    • Environmental Geology Group, School of GeosciencesUniversity of Sydney
  • Gavin Birch
    • Environmental Geology Group, School of GeosciencesUniversity of Sydney
  • Michael St. J. Warne
    • Centre for Environmental Contaminants ResearchCSIRO
  • Rick Krassoi
    • Ecotox Services Australasia Pty Ltd

DOI: 10.1007/s00128-009-9678-8

Cite this article as:
Hunt, J., Birch, G., Warne, M.S.J. et al. Bull Environ Contam Toxicol (2009) 82: 743. doi:10.1007/s00128-009-9678-8


This study evaluated the suitability of sealed containers for toxicity testing to prevent loss of volatile chlorinated hydrocarbons (VCHs) with a range of Australian marine organisms including: micro-algae; sea urchin and oyster larvae in 44 mL sealed vials and fish larvae; amphipods; and juvenile polychaetes in 1 L sealed jars. Vials prevented volatilisation of VCHs during testing. Jars were less effective, with average losses of 46%. Growth and development of algae, sea urchins and oysters in vials was acceptable, indicating suitability of the methodology. Jars were suitable for amphipods and polychaetes; however, further evaluation of the fish test is required.


Volatile chlorinated hydrocarbons Toxicity testing Sealed containers

Copyright information

© Springer Science+Business Media, LLC 2009