Nicotinamide mononucleotide protects against pro-inflammatory cytokine-mediated impairment of mouse islet function
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- Caton, P.W., Kieswich, J., Yaqoob, M.M. et al. Diabetologia (2011) 54: 3083. doi:10.1007/s00125-011-2288-0
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Nicotinamide phosphoribosyltransferase (NAMPT), the rate-limiting enzyme for NAD+ biosynthesis, exists as intracellular NAMPT (iNAMPT) and extracellular NAMPT (eNAMPT). eNAMPT, secreted from adipose tissue, promotes insulin secretion. Administration of nicotinamide mononucleotide (NMN), a product of the eNAMPT reaction, corrects impaired islet function in Nampt+/− mice. One of its potential targets is the NAD+-dependent deacetylase sirtuin 1. We hypothesised that altered NAMPT activity might contribute to the suppression of islet function associated with inflammation, and aimed to determine whether NMN could improve cytokine-mediated islet dysfunction.
Acute effects of NMN on cytokine-mediated islet dysfunction were examined in islets incubated with TNFα and IL1β, and in mice fed a fructose-rich diet (FRD) for 16 weeks. Changes in iNAMPT, eNAMPT and inflammation levels were determined in FRD-fed mice.
FRD-fed mice displayed markedly lower levels of circulating eNAMPT, with impaired insulin secretion and raised islet expression of Il1b. NMN administration lowered Il1b expression and restored suppressed insulin secretion in FRD-fed mice. NMN also restored insulin secretion in islets cultured with pro-inflammatory cytokines. The changes in islet function corresponded with changes in key markers of islet function and differentiation. The anti-inflammatory effects of NMN were partially blocked by inhibition of sirtuin 1.
Chronic fructose feeding causes severe islet dysfunction in mice. Onset of beta cell failure in FRD-fed mice may occur via lowered secretion of eNAMPT, leading to increased islet inflammation and impaired beta cell function. Administration of exogenous NMN to FRD-fed mice corrects inflammation-induced islet dysfunction. Modulation of this pathway may be an attractive target for amelioration of islet dysfunction associated with inflammation.
KeywordsAdipose tissue Fructose-rich diet IL1β Islet dysfunction Nicotinamide phosphoribosyltransferase TNFα
Brown adipose tissue
Control + vehicle
Control + nicotinamide mononucleotide
Extracellular nicotinamide phosphoribosyltransferase
Fructose-rich diet + nicotinamide mononucleotide
Fructose-rich diet + vehicle
Glucose-stimulated insulin secretion
Hanks’ buffered salt solution
Intracellular nicotinamide phosphoribosyltransferase
Leucine-stimulated insulin secretion
Nuclear factor κB
White adipose tissue
The prevalence of insulin resistance and type 2 diabetes has increased dramatically over the past four decades [1, 2]. However, as a consequence of pancreatic islet beta cell compensation, insulin resistance does not immediately lead to onset of type 2 diabetes . Only when pancreatic islet function becomes impaired, resulting in markedly decreased beta cell mass and suppressed glucose-stimulated insulin secretion (GSIS), does type 2 diabetes fully develop . The precise mechanisms responsible for pancreatic beta cell failure in type 2 diabetes are unclear; however, chronic inflammation plays a crucial role in the process [5, 6]. Type 2 diabetes and insulin resistance are progressive chronic inflammatory states and exposure to pro-inflammatory cytokines, such as IL1β and TNFα, leads to pancreatic beta cell death and suppressed insulin secretion [5, 6, 7]. Exposure of pancreatic beta cells to pro-inflammatory cytokines can occur via numerous sources, including secretion by resident or invading immune cells within the islet or as a result of exposure to circulating cytokines . In addition, exposure of islets to pro-inflammatory cytokines or high levels of NEFA and glucose (termed glucolipotoxicity) can induce IL1β and TNFα production within the pancreatic beta cells themselves .
Recent studies have demonstrated the importance of nicotinamide phosphoribosyltransferase (NAMPT) for pancreatic beta cell function . NAMPT catalyses the synthesis of nicotinamide mononucleotide (NMN) from nicotinamide and 5-phosphoribosyl-pyrophosphate. NMN in turn is converted to NAD+ by NMN adenylyltransferase . NAMPT exists in two forms, intracellular NAMPT (iNAMPT) and extracellular NAMPT (eNAMPT) . iNAMPT levels are high in brown adipose tissue (BAT), liver and kidney, intermediate in white adipose tissue (WAT), lung, spleen, testes and skeletal muscle, and undetectable in brain and pancreas . eNAMPT, thought to be produced through post-translational modification of iNAMPT, is released into plasma predominantly from adipose tissue, where it catalyses the synthesis of NMN . Due to the absence of iNAMPT in the pancreas, islets rely on circulating eNAMPT as a source of NAD+. Recent studies have highlighted the essential role of eNAMPT for GSIS. Nampt+/− mice display decreased circulating levels of eNAMPT and NMN, are glucose-intolerant and show impaired GSIS. Administration of NMN restores GSIS and improves glucose tolerance in these mice . The mechanism by which NMN corrects impaired GSIS has yet to be fully elucidated, but may involve sirtuin 1, a NAD+-dependent protein deacetylase that promotes GSIS [11, 12, 13]. Consistent with this, the phenotype of the beta-cell-specific Sirt1-overexpressing (BESTO) mouse, which shows enhanced GSIS, was found to be lost with increased age. This loss of phenotype with age correlated with reduced plasma NMN levels and was restored by NMN administration . Despite evidence suggesting that eNAMPT/NMN functions positively regulate beta cell function, the role of this pathway in insulin resistance and progression to impaired beta cell function has yet to be described.
Dietary sugar consumption has risen over recent decades, in parallel with increased precedence of the metabolic syndrome and type 2 diabetes, with the widespread use of high-fructose corn syrup contributing significantly to this increase [1, 2, 15]. Correspondingly, in rodent models, consumption of a fructose-rich diet (FRD) can lead to onset of aspects of the metabolic syndrome, including hyperglycaemia, dyslipidaemia and inflammation [16, 17, 18, 19, 20]. In humans, elevated intake of sugar-sweetened beverages is associated with an increased risk of the metabolic syndrome and type 2 diabetes [21, 22], whilst fructose consumption has been reported to worsen metabolic abnormalities in obese humans to a greater extent than glucose consumption [23, 24]. Fructose consumption has been linked to development of a pro-inflammatory phenotype in rodent models [18, 19, 25]. To explore the links between eNAMPT/NMN, inflammation and islet function, we investigated whether mice fed an FRD displayed an inflammatory phenotype and impaired islet function, and whether these could be rectified by NMN administration, thus implicating impaired NAMPT function as an underlying mechanism. In addition, we investigated whether NMN was protective against the negative effects of pro-inflammatory cytokines in isolated islets.
Male C57BL/6 mice aged 7 weeks (Charles River, Margate, UK) were fed an FRD (60% fructose) (TD.89247; Harlan Laboratories, Wyton, UK) or standard rodent diet (control) for 16 weeks. Subsets of FRD-fed mice were administered NMN (500 mg/kg body weight; i.p.)  or an equal volume of vehicle (saline) 16 h prior to tissue sampling to create four groups: (1) control + vehicle (CON+V); (2) control + NMN (CON+NMN); (3) FRD + vehicle (FRD+V); and (4) FRD+NMN. A separate group of 16 week FRD-fed mice were fasted for 24 h prior to tissue sampling. Islets were isolated as described below. Blood was collected in heparin-coated tubes and centrifuged to obtain plasma. BAT and WAT were snap-frozen in liquid nitrogen for analysis of protein levels and gene expression. Animal experiments were conducted in accordance with the Home Office regulations on the Operation of Animals (Scientific Procedures) Act 1986, published by HMSO, London. Animals were maintained on a 12 h light–dark cycle.
Blood chemistry analysis
Blood was collected between 09:00 and 11:00 hours. Plasma glucose (Thermo Electron, Melbourne, VIC, Australia) was analysed via colorimetric assay. Plasma insulin (Mercodia, Uppsala, Sweden) and eNAMPT (Phoenix Pharmaceuticals, Burlingame, CA, USA) concentrations were determined via ELISA.
Mouse pancreases were digested in 2 ml Hanks’ buffered salt solution (HBSS) containing collagenase P (1 mg/ml) and DNase I (0.15 mg/ml) (both from Roche Diagnostics, Burgess Hill, UK). Islets were hand-picked into RPMI 1640 medium containing 11 mmol/l glucose, supplemented with 10% (vol./vol.) heat-inactivated FBS, 100 U/ml penicillin and 100 μg/ml streptomycin (Sigma Aldrich, Poole, UK). Islets isolated from control mice were cultured for 24 h (37°C; 5% CO2) to allow recovery from isolation. After 24 h, islets were transferred to fresh RPMI medium and incubated with the following treatments for 48 h, before RNA and protein extraction or ex vivo islet function assays. Treatments for 48 h were as follows: IL1β (5 ng/ml) and TNFα (10 ng/ml); palmitate (100 μmol/l); NMN (100 μmol/l); co-incubation with IL1β (5 ng/ml), TNFα (10 ng/ml) and NMN (100 μmol/l); co-incubation with IL1β (5 ng/ml), TNFα (10 ng/ml), NMN (100 μmol/l) and the specific sirtuin 1 inhibitor EX-527 (10 μmol/l); or co-incubation with palmitate (100 μmol/l) and NMN (100 μmol/l). Palmitate was initially dissolved in ethanol before being complexed with BSA (10% wt/vol.). Final concentrations of ethanol (0.5%, vol./vol.) and BSA (1%, wt/vol.) were not toxic to islets. Islets isolated from FRD-fed mice were either picked into RPMI and immediately lysed for RNA extraction, or transferred to RPMI and allowed to recover for 2 h, prior to ex vivo insulin secretion assay.
Insulin secretion ex vivo
For islet insulin secretion assays, batches of eight size-matched islets were pre-incubated for 1 h at 37°C in HBSS containing 3 mmol/l glucose, 10 mmol/l HEPES (pH 7.4) and 0.2% BSA (wt/vol.). For GSIS, islets were incubated for 1 h at 37°C in HBSS (10 mmol/l HEPES [pH 7.4], 0.2% BSA) supplemented with 3 or 17 mmol/l glucose. For leucine-stimulated insulin secretion (LSIS), islets were incubated for 1 h at 37°C in HBSS (3 mmol/l glucose, 10 mmol/l HEPES [pH 7.4], 0.2% BSA) supplemented with 2 or 20 mmol/l leucine. After 1 h, media were collected for determination of insulin levels.
Gene expression was measured by quantitative RT-PCR, using Sybr Green methodology (Invitrogen, Paisley, UK). Gene expression was determined by standard curve methodology, normalised against TaqMan 18S ribosomal RNA (Applied Biosystems, Warrington, UK). Changes in gene expression are represented as fold change relative to 1, where control equals 1. For primer and probe details (Eurogentec, Southampton, UK for all), see electronic supplementary material (ESM) Table 1.
Solubilised protein samples (2–10 μg) that had been measured and equalised in each fraction (RC-DC System; Bio-Rad, Hemel Hempstead, UK) were separated by SDS-PAGE and transferred on to polyvinylidene difluoride membrane (GE Healthcare, Amersham, UK). Blots were blocked for 1 h in 5% (wt/vol.) milk protein in Tris-buffered saline/0.1% Tween-20 (vol./vol.) (TBS/T) solution and then incubated overnight in anti-NAMPT (Sigma) or anti-IL1β (Abcam, Cambridge, UK) primary antibody. Detection of bands was achieved by chemiluminescence substrate (SuperSignal West Pico; Pierce, Rockford, IL, USA). Reference protein measurements were made with mouse monoclonal anti-β-actin (clone AC-15) primary antibody in a 3% (wt/vol.) milk/TBS-T solution, at 4°C.
Results are expressed as mean±SEM. Statistical comparisons were obtained using GraphPad (GraphPad Software, La Jolla, CA, USA). Statistical differences were calculated using a paired t test or one-way ANOVA followed by Bonferroni’s post test where appropriate.
FRD-fed mice display a pro-inflammatory phenotype with elevated blood glucose
FRD increases iNAMPT abundance in adipose tissue while suppressing circulating eNAMPT concentrations
Insulin secretion is suppressed in FRD-fed mice
NMN administration protects against islet dysfunction in FRD-fed mice
Since Nampt+/− mice show impaired islet function , we reasoned that the suppressed eNAMPT levels observed in FRD-fed mice might play a role in the onset of islet dysfunction in these mice. To further examine this, we investigated whether the reaction product of eNAMPT, NMN, provided protection in vivo against beta cell dysfunction in FRD-fed mice. NMN (500 mg/kg body weight; i.p.) [9, 14] was administered to FRD-fed mice 16 h prior to islet isolation. The effects of NMN administration in vivo on insulin secretion ex vivo were first examined with islets isolated from control mice maintained on a standard diet (CON+NMN group). Islets isolated from CON+NMN mice displayed elevated GSIS (twofold; p < 0.01) (Fig. 3c) compared with CON+V mice (which had been injected with saline). Similarly, LSIS was also modestly but significantly increased in CON+NMN mice (37 ± 8%) compared with CON+V mice (Fig. 3d). NMN had no effect on basal rates of insulin secretion measured at 3 mmol/l glucose or 2 mmol/l leucine (Fig. 3c, d). Significantly, in vivo administration of NMN abolished the suppressive effects of FRD on GSIS and LSIS (Fig. 3e, f). Whereas NMN administration to FRD-fed mice completely restored LSIS ex vivo (Fig. 3f), GSIS was significantly elevated above rates seen in CON+V mice (Fig. 3e) and also greatly exceeded those of CON+NMN mice.
NMN protects against pro-inflammatory cytokine-mediated islet dysfunction
Glucolipotoxicity, which results from elevated circulating levels of glucose and NEFA such as palmitate, can also lead to suppression of islet insulin secretion [26, 27, 28], in part through induction of Il1b expression [5, 6, 29]. Consistent with this, exposure of islets to palmitate (100 μmol/l) and glucose (20 mmol/l) in combination for 48 h induced Il1b mRNA (Fig. 4g), and suppressed GSIS and LSIS (Fig. 4h, i). Similarly to the effects seen in cytokine-exposed islets, the effects of palmitate/glucose were reversed by co-incubation with NMN (Fig. 4h, i).
NMN reverses FRD and pro-inflammatory cytokine-mediated changes in expression of genes encoding islet markers
Similarly, isolated islets incubated with IL1β/TNFα displayed reduced expression of Pdx1 (72%; p < 0.001), Glut2 (90%; p < 0.01) and Gk (42%; p < 0.05), as well as increased expression of Inos (3.9-fold; p < 0.001) and Bax (twofold; p < 0.001). These changes in gene expression elicited by IL1β/TNFα were reversed by co-incubation with NMN (ESM Fig. 1a–e). Moreover, the IL1β/TNFα-mediated induction of Il1b gene expression and production of the corresponding protein that are described above were also suppressed by NMN (ESM Fig. 1f), supporting the notion of an anti-inflammatory mechanism of NMN action. Expression of two other islet transcription factors, Tfam and Hnf1a, were unchanged by IL1β/TNFα and/or NMN (data not shown). These changes in gene expression in response to pro-inflammatory cytokines and NMN are reminiscent of those observed in islets isolated from FRD-fed mice.
Taken together, these data indicate that NMN improves islet function in FRD-fed mice in association with beneficial changes in expression of genes involved in glucometabolic, anti-inflammatory and apoptotic processes.
NMN-mediated induction of insulin secretion involves sirtuin 1
We next assessed a possible role for sirtuin 1 as a target mediating the actions of NMN in islets. Consistent with a potential role for sirtuin 1 in mediating the effects of NMN, expression of Sirt1 was suppressed by 65% in FRD+V mice (p < 0.05) (Fig. 5h). In addition, FRD+V mice displayed decreased mRNA levels of the mitochondrial sirtuin, Sirt3 (29%; p < 0.05) (Fig. 5i). The role of sirtuin 3 in islet function is not yet known, but it is known to positively regulate mitochondrial ATP production [33, 34, 35], which is important for nutrient-stimulated insulin secretion, and to be induced by NAMPT [36, 37]. These effects were reversed by NMN in FRD+NMN mice (Fig. 5h, i). Similarly, IL1β/TNFα treatment led to a 46% (p < 0.01) reduction in Sirt1 mRNA and a 54% (p < 0.001) decrease in Sirt3 mRNA. Co-incubation of NMN with TNFα/IL1β restored Sirt1 and Sirt3 expression to control levels (ESM Fig. 2a, b). Consistent with the notion of a mechanistic role for sirtuin 1 in mediating the effects of NMN, co-incubation of islets with the specific sirtuin 1 inhibitor EX-527 blocked the enhancing effects of NMN alone upon insulin secretion (ESM Fig. 2c). We next investigated whether EX-527 could inhibit the effect of NMN on cytokine-mediated islet dysfunction. The effects of NMN in restoring TNFα/IL1β-mediated suppression of GSIS were partially blocked by EX-527 (45%) (ESM Fig. 2d), suggesting that sirtuin 1 mediates approximately 55% of the protective effects of NMN against cytokine-mediated impairment of GSIS.
Previous studies have described a role for pro-inflammatory cytokines in the mediation of beta cell failure [5, 6], a key factor in the progression from insulin resistance to type 2 diabetes. Separately, studies using Nampt+/− mice have highlighted a role for eNAMPT and its reaction product NMN in enhancing pancreatic beta cell function . Here, we report suppressed insulin secretion in response to glucose and leucine in islets cultured with the inflammatory cytokines IL1β and TNFα. Importantly, IL1β/TNFα-mediated suppression of beta cell function was reversed by co-incubation with NMN, indicating that NMN may protect against beta cell failure through an anti-inflammatory mechanism. IL1β can induce its own production through autoinflammatory mechanisms [5, 6]. Consistent with an anti-inflammatory role, co-incubation with NMN reversed IL1β/TNFα-mediated increases in Il1b expression and IL1β abundance.
Increased consumption of diets rich in sugar, particularly fructose, have been linked to the development of type 2 diabetes [21, 22]. We report here that FRD-fed mice displayed dramatically impaired islet insulin secretion compared with control, together with the development of fasting hyperglycaemia and hyperinsulinaemia, as well as islet and adipose tissue inflammation. We therefore further explored the links between NMN and inflammation in vivo in mice fed an FRD. Decreased plasma eNAMPT levels in FRD-fed mice were associated with marked suppression of insulin secretion and elevated islet Il1b expression, both of which were reversed by administration of NMN.
Therefore we propose that adequate tissue delivery and uptake of eNAMPT/NMN are essential for preservation of beta cell function during insulin resistance and that the protective effects of eNAMPT/NMN may occur in part through suppressed expression of genes involved in inflammation. In contrast, during beta cell failure, a decline in eNAMPT/NMN uptake results in loss of protection against chronic inflammation and a consequent rise of IL1β levels, with eventual beta cell death and suppressed insulin secretion.
Further studies will be required to test this hypothesis, with regard to the effects of eNAMPT/NMN on glycaemia and the resolution of insulin resistance, by investigating the impact of NMN administration on insulin and glucose levels during a glucose tolerance test. However, given the positive effects of NMN administration on the resolution of insulin resistance in transgenic models [9, 14], it seems likely that NMN will improve insulin resistance in models of diet-induced insulin resistance. In addition, measurements of plasma NMN are required in models of insulin resistance and type 2 diabetes. NMN measurements were carried out during this study using HPLC; however, these measurements proved to be inconclusive due to variability within groups (data not shown), and a larger study will be required to accurately establish the relationship between eNAMPT, islet dysfunction and NMN.
Interestingly, patients homozygous for either of two single nucleotide polymorphisms in the NAMPT promoter display lower plasma insulin levels, suggesting a connection between NAMPT dysfunction and regulation of insulin secretion in humans . Based on our studies of the effects of NMN on insulin secretion in vitro, it is plausible that elevation of eNAMPT/NMN levels may be part of the mechanism allowing beta cell compensation. We predict that this mechanism maintains insulin secretion by providing protection against chronic inflammation through lowering of islet IL1β levels, which may otherwise be increased. Further studies will be required to assess whether these changes are unique to fructose consumption, but given recent increases in dietary sugar consumption, the results described here are likely to be of relevance in humans. Consistent with the present findings in mice maintained on an FRD for 16 weeks, another study has described increased beta cell apoptosis in rats fed an FRD for 3 weeks , but with increased insulin secretion, suggesting that the adverse effects of fructose feeding on islet function is progressive. Further studies will elucidate how these progressively deleterious changes relate to alterations in eNAMPT regulation.
Interestingly, lower plasma levels of eNAMPT/NMN in FRD-fed mice corresponded with increased abundance of iNAMPT in BAT and, to a lesser extent, in WAT. This suggests that lower eNAMPT levels in FRD-fed mice occur through a defect either in eNAMPT secretion, or in the putative post-translational modification  that may process iNAMPT for secretion.
In summary, we provide evidence for a novel mechanism that mediates onset of beta cell failure in dietary-induced inflammation via reduced exposure to eNAMPT, leading to increased islet inflammation and impaired beta cell function. Moreover, we show that NMN, an intermediary in the NAMPT reaction, can correct cytokine-induced islet dysfunction, suggesting that modulation of this pathway could be an attractive target for treatment of islet inflammation in type 2 diabetes. Finally, this study provides further evidence of the dangers of consumption of a sugar-rich diet, and may have important implications regarding the pathophysiology of type 2 diabetic patients.
This research was supported by Diabetes UK grants BDA:RD08/0003665, BDA:RD06/0003424 and BDA:RD03/0002725. We thank N.K. Nayuni (William Harvey Research Institute, Queen Mary University of London) for help with HPLC measurements of NMN.
PWC, JK, MMY, MJH and MCS were responsible for the conception and design, or analysis and interpretation of data. PWC, JK, MJH and MSC contributed to the experimental work. PWC, JK, MMY, MJH and MSC drafted the article or revised it critically. PWC, JK, MMY, MJH and MSC gave final approval of the version to be published.
Duality of interest
The authors declare that there is no duality of interest associated with this manuscript.