Enterovirus infection in human pancreatic islet cells, islet tropism in vivo and receptor involvement in cultured islet beta cells
- P. YlipaastoAffiliated withEnterovirus Laboratory, National Public Health Institute
- , K. KlingelAffiliated withDepartment of Molecular Pathology, University Hospital Tübingen
- , A. M. LindbergAffiliated withDepartment of Chemistry and Biomedical Sciences, University of Kalmar
- , T. OtonkoskiAffiliated withHospital for Children and Adolescents and Program of Developmental and Reproductive Biology, Biomedicum, University of Helsinki
- , R. KandolfAffiliated withDepartment of Molecular Pathology, University Hospital Tübingen
- , T. HoviAffiliated withEnterovirus Laboratory, National Public Health Institute
- , M. RoivainenAffiliated withEnterovirus Laboratory, National Public Health Institute Email author
It is thought that enterovirus infections cause beta-cell damage and contribute to the development of Type 1 diabetes by replicating in the pancreatic islets. We sought evidence for this through autopsy studies and by investigating known enterovirus receptors in cultured human islets.
Autopsy pancreases from 12 newborn infants who died of fulminant coxsackievirus infections and from 65 Type 1 diabetic patients were studied for presence of enteroviral ribonucleic acid by in situ hybridisation. Forty non-diabetic control pancreases were included in the study. The expression and role of receptor candidates in cultured human islets were investigated with receptor-specific antibodies using immunocytochemistry and functional assays.
Enterovirus-positive islet cells were found in some of both autopsy specimen collections, but not in control pancreases. No infected cells were seen in exocrine tissue. The cell surface molecules, poliovirus receptor and integrin αvβ3, which act as enterovirus receptors in established cell lines, were expressed in beta cells. Antibodies to poliovirus receptor, human coxsackievirus and adenovirus receptor and integrin αvβ3 protected islets and beta cells from adverse effects of poliovirus, coxsackie B viruses, and several of the arginine-glycine-aspartic acid motifs containing enteroviruses and human parechovirus 1 respectively. No evidence was found for expression of the decay-accelerating factor which acts as a receptor for several islet-cell-replicating echoviruses in established cell lines.
The results show a definite islet-cell tropism of enteroviruses in the human pancreas. Some enteroviruses seem to use previously identified cell surface molecules as receptors in beta cells, whereas the identity of receptors used by other enteroviruses remains unknown.
KeywordsEnterovirus coxsackievirus echovirus poliovirus human pancreas islets beta cells receptors
- Enterovirus infection in human pancreatic islet cells, islet tropism in vivo and receptor involvement in cultured islet beta cells
Volume 47, Issue 2 , pp 225-239
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- Author Affiliations
- 1. Enterovirus Laboratory, National Public Health Institute, Mannerheimintie 166, 00300, Helsinki, Finland
- 2. Department of Molecular Pathology, University Hospital Tübingen, Tübingen, Germany
- 3. Department of Chemistry and Biomedical Sciences, University of Kalmar, Kalmar, Sweden
- 4. Hospital for Children and Adolescents and Program of Developmental and Reproductive Biology, Biomedicum, University of Helsinki, Helsinki, Finland