Original Paper

Theoretical and Applied Genetics

, Volume 102, Issue 2, pp 177-186

Molecular linkage map for an intraspecific recombinant inbred population of durum wheat (Triticum turgidum L. var. durum)

  • M. M. NachitAffiliated withICARDA, CIMMYT/ICARDA, Durum Improvement Program, P.O. Box 5466, Aleppo, Syria e-mail: M.Nachit@CGIAR.ORG
  • , I. ElouafiAffiliated withICARDA, CIMMYT/ICARDA, Durum Improvement Program, P.O. Box 5466, Aleppo, Syria e-mail: M.Nachit@CGIAR.ORG
  • , A. PagnottaAffiliated withDipartimento di Agrobiologia e Agrochimica, Università della Tuscia, Via S. Camillo de Lellis, 01100 Viterbo, Italy
  • , A. El SalehAffiliated withICARDA, CIMMYT/ICARDA, Durum Improvement Program, P.O. Box 5466, Aleppo, Syria e-mail: M.Nachit@CGIAR.ORG
  • , E. IaconoAffiliated withDipartimento di Agrobiologia e Agrochimica, Università della Tuscia, Via S. Camillo de Lellis, 01100 Viterbo, Italy
  • , M. LabhililiAffiliated withICARDA, CIMMYT/ICARDA, Durum Improvement Program, P.O. Box 5466, Aleppo, Syria e-mail: M.Nachit@CGIAR.ORG
  • , A. AsbatiAffiliated withICARDA, CIMMYT/ICARDA, Durum Improvement Program, P.O. Box 5466, Aleppo, Syria e-mail: M.Nachit@CGIAR.ORG
  • , M. AzrakAffiliated withICARDA, CIMMYT/ICARDA, Durum Improvement Program, P.O. Box 5466, Aleppo, Syria e-mail: M.Nachit@CGIAR.ORG
  • , H. HazzamAffiliated withICARDA, CIMMYT/ICARDA, Durum Improvement Program, P.O. Box 5466, Aleppo, Syria e-mail: M.Nachit@CGIAR.ORG
    • , D. BenscherAffiliated withDepartment of Plant Breeding, Cornell University, Ithaca, NY14853, USA
    • , M. KhairallahAffiliated withCIMMYT, Applied Biotechnology Center, Lisboa 27, A.P. 6–641, 06600 Mexico, D.F. Mexico
    • , J.-M. RibautAffiliated withCIMMYT, Applied Biotechnology Center, Lisboa 27, A.P. 6–641, 06600 Mexico, D.F. Mexico
    • , O. A. TanzarellaAffiliated withDipartimento di Agrobiologia e Agrochimica, Università della Tuscia, Via S. Camillo de Lellis, 01100 Viterbo, Italy
    • , E. PorcedduAffiliated withDipartimento di Agrobiologia e Agrochimica, Università della Tuscia, Via S. Camillo de Lellis, 01100 Viterbo, Italy
    • , M. E. SorrellsAffiliated withDepartment of Plant Breeding, Cornell University, Ithaca, NY14853, USA

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract 

Durum wheat (Triticum turgidum L. var. durum) is an economically and nutritionally important cereal crop in the Mediterranean region. To further our understanding of durum genome organization we constructed a durum linkage map using restriction fragment length polymorphisms (RFLPs), simple sequence repeats (SSRs) known as Gatersleben wheat microsatellites (GWMs), amplified fragment length polymorphisms (AFLPs), and seed storage proteins (SSPs: gliadins and glutenins). A population of 110 F9 recombinant inbred lines (RILs) was derived from an intraspecific cross between two durum cultivars, Jennah Khetifa and Cham 1. The two parents exhibit contrasting traits for resistance to biotic and abiotic stresses and for grain quality. In total, 306 markers have been placed on the linkage map – 138 RFLPs, 26 SSRs, 134 AFLPs, five SSPs, and three known genes (one pyruvate decarboxylase and two lipoxygenases). The map is 3598 cM long, with an average distance between markers of 11.8 cM, and 12.1% of the markers deviated significantly from the expected Mendelian ratio 1:1. The molecular markers were evenly distributed between the A and B genomes. The chromosome with the most markers is 1B (41 markers), followed by 3B and 7B, with 25 markers each. The chromosomes with the fewest markers are 2A (11 markers), 5A (12 markers), and 4B (15 markers). In general, there is a good agreement between the map obtained and the Triticeae linkage consensus maps. This intraspecific map provides a useful tool for marker-assisted selection and map-based breeding for resistance to biotic and abiotic stresses and for improvement of grain quality.

Keywords Durum RFLP SSR AFLP Seed storage proteins Genetic linkage mapping