Theoretical and Applied Genetics

, Volume 98, Issue 6, pp 1138–1145

Over-expression of the cloned rice thaumatin-like protein (PR-5) gene in transgenic rice plants enhances environmental friendly resistance to Rhizoctonia solani causing sheath blight disease

Authors

  • K. Datta
    • Plant Breeding, Genetics and Biochemistry Division, International Rice Research Institute, P.O. Box 3127, 1271 Makati City, Philippines Fax: +63-2-8911292 E-mail: sdatta@irrl.cgnet.com; sdatta@irrl.cgiar.org
  • R. Velazhahan
    • Department of Biochemistry, Kansas State University, Manhattan, KS 66506-3702, USA
  • N. Oliva
    • Plant Breeding, Genetics and Biochemistry Division, International Rice Research Institute, P.O. Box 3127, 1271 Makati City, Philippines Fax: +63-2-8911292 E-mail: sdatta@irrl.cgnet.com; sdatta@irrl.cgiar.org
  • I. Ona
    • Entomology and Plant Pathology Division, International Rice Research Institute, P.O. Box 933, Manila 1099, Philippines
  • T. Mew
    • Entomology and Plant Pathology Division, International Rice Research Institute, P.O. Box 933, Manila 1099, Philippines
  • G. S. Khush
    • Plant Breeding, Genetics and Biochemistry Division, International Rice Research Institute, P.O. Box 3127, 1271 Makati City, Philippines Fax: +63-2-8911292 E-mail: sdatta@irrl.cgnet.com; sdatta@irrl.cgiar.org
  • S. Muthukrishnan
    • Department of Biochemistry, Kansas State University, Manhattan, KS 66506-3702, USA
  • S. K. Datta
    • Plant Breeding, Genetics and Biochemistry Division, International Rice Research Institute, P.O. Box 3127, 1271 Makati City, Philippines Fax: +63-2-8911292 E-mail: sdatta@irrl.cgnet.com; sdatta@irrl.cgiar.org

DOI: 10.1007/s001220051178

Cite this article as:
Datta, K., Velazhahan, R., Oliva, N. et al. Theor Appl Genet (1999) 98: 1138. doi:10.1007/s001220051178

Abstract

 A 1.1-kb DNA fragment containing the coding region of a thaumatin-like protein (TLP-D34), a member of the PR-5 group, was cloned into the rice transformation vector pGL2, under the control of the CaMV 35S promoter. The Indica rice cultivars, ‘Chinsurah Boro II’, ‘IR72’, and ‘IR51500’ were transformed with the tlp gene construct by PEG-mediated direct gene transfer to protoplasts and by biolistic transformation using immature embryos. The presence of the chimeric gene in T0, T1, and T2 transgenic plants was detected by Southern blot analysis. The presence of the expected 23-kDa TLP in transgenic plants was confirmed by Western blot analysis and by staining with Coomassie Brilliant Blue. Bioassays of transgenic plants challenged with the sheath blight pathogen, Rhizoctonia solani, indicated that over-expression of TLP resulted in enhanced resistance compared to control plants.

Key words Thaumatin-like proteinPR-5Rhizoctonia solaniSheath blight diseaseRice
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© Springer-Verlag Berlin Heidelberg 1999