Theoretical and Applied Genetics

, Volume 98, Issue 6, pp 1091-1098

Abundance and polymorphism of microsatellite markers in the tea tree (Melaleuca alternifolia, Myrtaceae)

  • M. RossettoAffiliated withCentre for Plant Conservation Genetics, Southern Cross University, PO Box 157 Lismore, NSW 2480, Australia Fax: +61-2-6622 2080 E-mail: mrossett@scu.edu.au
  • , A. McLauchlanAffiliated withCentre for Plant Conservation Genetics, Southern Cross University, PO Box 157 Lismore, NSW 2480, Australia Fax: +61-2-6622 2080 E-mail: mrossett@scu.edu.au
  • , F. C. L. HarrissAffiliated withCentre for Plant Conservation Genetics, Southern Cross University, PO Box 157 Lismore, NSW 2480, Australia Fax: +61-2-6622 2080 E-mail: mrossett@scu.edu.au
  • , R. J. HenryAffiliated withCentre for Plant Conservation Genetics, Southern Cross University, PO Box 157 Lismore, NSW 2480, Australia Fax: +61-2-6622 2080 E-mail: mrossett@scu.edu.au
  • , P. R. BaverstockAffiliated withCentre for Animal Conservation Genetics, Southern Cross University, PO Box 157 Lismore, NSW 2480, Australia
  • , L. S. LeeAffiliated withCentre for Plant Conservation Genetics, Southern Cross University, PO Box 157 Lismore, NSW 2480, Australia Fax: +61-2-6622 2080 E-mail: mrossett@scu.edu.au
  • , T. L. MaguireAffiliated withCentre for Coastal Management, Southern Cross University, PO Box 157 Lismore, NSW 2480, Australia
  • , K. J. EdwardsAffiliated withIACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol, BS18 9AF, UK

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Abstract

 The sequencing of 831 clones from an enriched microsatellite library of Melaleuca alternifolia (Myrtaceae) yielded 715 inserts containing repeat motifs. The majority of these (98%) were dinucleotide repeats or trinucleotide repeats averaging 22 and 8 repeat motifs respectively. The AG/GA motif was the most common, accounting for 43% of all microsatellites. From a total of 139 primer pairs designed, 102 produced markers within the expected size range. The majority of these (93) were polymorphic. Primer pairs were tested on five selected M. alternifolia genotypes. Loci based on dinucleotide repeats detected on average a greater number of alleles (4.2) than those based on trinucleotide repeats (2.9). The loci described will provide a large pool of polymorphisms useful for population studies, genetic mapping, and possibly application in other Myrtaceae.

Key words Simple sequence repeats Enriched microsatellite library Molecular marker Myrtaceae Melaleuca