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FaRCg1: a quantitative trait locus conferring resistance to Colletotrichum crown rot caused by Colletotrichum gloeosporioides in octoploid strawberry

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Abstract

Colletotrichum crown rot (CCR) is an important disease of strawberry (Fragaria ×ananassa) throughout the Southeastern US and in subtropical climates around the world, where hot and humid conditions facilitate rapid disease development. Yet no resistance loci have been described to date, as genetic studies have been historically difficult in allo-octoploid (2n = 8x = 56) strawberry. In the present study, we investigate the genetic architecture of resistance to CCR. Four population sets from the University of Florida were inoculated in four different seasons from 2013–2014 to 2016–2017. Two large, multiparental discovery population sets were used for QTL discovery, and two validation sets of cultivars and advanced selections representing the parent pool of the breeding program were also assessed. Subgenome-specific single-nucleotide polymorphism (SNP) markers were mapped, and FlexQTL™ software was utilized to perform a Bayesian, pedigree-based QTL analysis. A quantitative trait locus on linkage group 6B, which we name FaRCg1, accounts for most of the genetic variation for resistance in the discovery sets (26.8–29.8% in 2013–2014 and 17% in 2015–2016). High-throughput marker assays were developed for the most significant SNPs which correlated with the mode of the QTL region. The discovery and characterization of the FaRCg1 locus and the molecular tools developed from it will be utilized to achieve increased genetic gains for resistance.

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Acknowledgements

The authors acknowledge the strawberry breeding team at the Gulf Coast Research and Education Center for their assistance. Financial support for this project was provided through the Florida Strawberry Growers Association (FSGA) and the U.S. Department of Agriculture/National Institute of Food and Agriculture Specialty Crop Research Initiative project ‘RosBREED: Combining disease resistance with horticultural quality in new rosaceous cultivars’ under Award Number 2014-51181-22378.

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Authors and Affiliations

  1. Department of Horticultural Science, Institute of Food and Agricultural Services, Gulf Coast Research and Education Center, University of Florida, Wimauma, FL, 33598, USA

    Ashlee Anciro, Jozer Mangandi, Sujeet Verma, Vance M. Whitaker & Seonghee Lee

  2. Department of Plant Pathology, Institute of Food and Agricultural Services, Gulf Coast Research and Education Center, University of Florida, Wimauma, FL, 33598, USA

    Natalia Peres

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  1. Ashlee Anciro
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  2. Jozer Mangandi
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  6. Seonghee Lee
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Contributions

AA performed phenotyping and analysis for the 2015–2016 and 2016–2017 seasons and prepared the initial manuscript; JM performed phenotyping and analysis for the 2013–2014 and 2014–2015 seasons; SV prepared the linkage map, provided guidance for QTL analysis, and helped with re-analysis of the 2013–2014 season; SL provided project and HRM guidance and assistance; VW initiated the research and provided project as well as analysis guidance. All authors have reviewed and edited the manuscript.

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Correspondence to Seonghee Lee.

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This article does not contain any studies with human or animal subjects.

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Communicated by Christine A Hackett.

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Anciro, A., Mangandi, J., Verma, S. et al. FaRCg1: a quantitative trait locus conferring resistance to Colletotrichum crown rot caused by Colletotrichum gloeosporioides in octoploid strawberry. Theor Appl Genet 131, 2167–2177 (2018). https://doi.org/10.1007/s00122-018-3145-z

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  • DOI: https://doi.org/10.1007/s00122-018-3145-z

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