Abstract
Key message
A high-resolution genetic map that was constructed for the Lf1 -residing region will provide valuable information for map-based cloning and genetic improvement efforts in soybean.
Abstract
Changes in leaf architecture as photosynthesis factories remain a major challenge for the improvement of crop productivity. Unlike most soybeans, which have compound leaves comprising three leaflets, the soybean Lf1 mutant has a high frequency of compound leaves with five leaflets in a partially dominant manner. Here, we generated a fine genetic map to determine the genetic basis of this multifoliolate leaf trait. A five-leaflet variant Dusam was found in a recently collected landrace cultivar. Phenotypic data were collected from the F2 population of a cross between the Dusam and three-leaflet cultivar V94-5152. The mapping results generated using public markers indicated that the five-leaflet determining gene in Dusam is an allele of the previously studied Lf1 gene on chromosome 8. A high-resolution map delimited the genomic region controlling the leaflet number trait to a sequence length of 49 kb. AP2 domain-containing Glyma.08g281900 annotated in this 49 kb region appeared to be a strong candidate for the Lf1-encoding gene, as members of the AP2-type transcription factor family regulate lateral organ development. Dusam additionally exhibits visually distinct phenotypes for shattering and seed-coat cracking traits. However, the two traits were clearly unlinked to the Lf1 gene in our mapping population. Interestingly, the mapping results suggest that the Lf1 gene most likely exerts a pleiotropic effect on the number of seeds per pod. Thus, our results provide a strong foundation towards the cloning of this compound leaf development gene and marker-assisted selection of the seeds per pod trait.
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Acknowledgements
This study was supported principally supported by the National Research Foundation of Korea grant funded by the Korea government (MSIP) (No. 2015R1A2A2A01002969) and partly by a grant from the Next-Generation BioGreen 21 Program (PJ011015032017), Rural Development Administration, and by the Korea Research Institute of Bioscience and Biotechnology (KRIBB) Research Initiative Program. We thank Dr. Suk Yoon Kwon at KRIBB for collecting the landrace soybean seeds from Dangjin, Korea.
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Communicated by Henry T. Nguyen.
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Jeong, SC., Kim, J.H. & Bae, D.N. Genetic analysis of the Lf1 gene that controls leaflet number in soybean. Theor Appl Genet 130, 1685–1692 (2017). https://doi.org/10.1007/s00122-017-2918-0
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DOI: https://doi.org/10.1007/s00122-017-2918-0