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QTL analysis of cotton fiber length in advanced backcross populations derived from a cross between Gossypium hirsutum and G. mustelinum

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QTLs for fiber length mapped in three generations of advanced backcross populations derived from crossing Gossypium hirsutum and Gossypium mustelinum showed opportunities to improve elite cottons by introgression from wild relatives.

Abstract

The molecular basis of cotton fiber length in crosses between Gossypium hirsutum and Gossypium mustelinum was dissected using 21 BC3F2 and 12 corresponding BC3F2:3 and BC3F2:4 families. Sixty-five quantitative trait loci (QTLs) were detected by one-way analysis of variance. The QTL numbers detected for upper-half mean length (UHM), fiber uniformity index (UI), and short fiber content (SFC) were 19, 20, and 26 respectively. Twenty-three of the 65 QTLs could be detected at least twice near adjacent markers in the same family or near the same markers across different families/generations, and 32 QTLs were detected in both one-way variance analyses and mixed model-based composite interval mapping. G. mustelinum alleles increased UHM and UI and decreased SFC for five, one, and one QTLs, respectively. In addition to the main-effect QTLs, 17 epistatic QTLs were detected which helped to elucidate the genetic basis of cotton fiber length. Significant among-family genotypic effects were detected at 18, 16, and 16 loci for UHM, UI, and SFC, respectively. Six, two, and two loci showed genotype × family interaction for UHM, UI and SFC, respectively, illustrating complexities that might be faced in introgression of exotic germplasm into cultivated cotton. Co-location of many QTLs for UHM, UI, and SFC accounted for correlations among these traits, and selection of these QTLs may improve the three traits simultaneously. The simple sequence repeat (SSR) markers associated with G. mustelinum QTLs will assist breeders in transferring and maintaining valuable traits from this exotic source during cultivar development.

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Acknowledgements

We appreciate financial help from the National Science Foundation (PFI award IIP 0917856; AIR award IIP 1127755), Cotton Incorporated, and Key Research and Development Project of Jiangsu Province, China (Modern Agriculture, BE2015353). We thank Jennifer McCurdy for help in field work and Cornelia Lemke for technical assistance in the lab.

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Author notes

  1. O. Lloyd May

    Present address: Monsanto Cotton Breeding, Tifton, GA, 31793, USA

Authors and Affiliations

  1. Plant Genome Mapping Laboratory, University of Georgia, 111 Riverbend Road, Athens, GA, 30602, USA

    Baohua Wang, Zhengsheng Zhang, Min Liu & Andrew H. Paterson

  2. School of Life Sciences, Nantong University, Nantong, 226019, Jiangsu, China

    Baohua Wang & Zhimin Zhuang

  3. Department of Crop and Soil Sciences, University of Georgia, 2356 Rainwater Road, Tifton, GA, 31793, USA

    Baohua Wang, Zhimin Zhuang, Edward L. Lubbers, O. Lloyd May & Peng W. Chee

  4. Université catholique de Louvain, Place Croix du Sud 2/11, 1348, Louvain-la-Neuve, Belgium

    Xavier Draye

  5. Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, Chongqing, 400716, China

    Zhengsheng Zhang

  6. Cotton Incorporated, Cary, NC, 27513, USA

    Don Jones

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  1. Baohua Wang
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Communicated by Joshua A. Udall.

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Wang, B., Draye, X., Zhuang, Z. et al. QTL analysis of cotton fiber length in advanced backcross populations derived from a cross between Gossypium hirsutum and G. mustelinum . Theor Appl Genet 130, 1297–1308 (2017). https://doi.org/10.1007/s00122-017-2889-1

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