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Identification of Pm58 from Aegilops tauschii

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Abstract

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A novel powdery mildew-resistance gene, designated Pm58, was introgressed directly from Aegilops tauschii to hexaploid wheat, mapped to chromosome 2DS, and confirmed to be effective under field conditions. Selectable KASP™ markers were developed for MAS.

Abstract

Powdery mildew caused by Blumeria graminis (DC.) f. sp. tritici (Bgt) remains a significant threat to wheat (Triticum aestivum L.) production. The rapid breakdown of race-specific resistance to Bgt reinforces the need to identify novel sources of resistance. The d-genome species, Aegilops tauschii, is an excellent source of disease resistance that is transferrable to T. aestivum. The powdery mildew-resistant Ae. tauschii accession TA1662 (2n = 2x = DD) was crossed directly with the susceptible hard white wheat line KS05HW14 (2n = 6x = AABBDD) followed by backcrossing to develop a population of 96 BC2F4 introgression lines (ILs). Genotyping-by-sequencing was used to develop a genome-wide genetic map that was anchored to the Ae. tauschii reference genome. A detached-leaf Bgt assay was used to screen BC2F4:6 ILs, and resistance was found to segregate as a single locus (χ = 2.0, P value = 0.157). The resistance gene, referred to as Pm58, mapped to chromosome 2DS. Pm58 was evaluated under field conditions in replicated trials in 2015 and 2016. In both years, a single QTL spanning the Pm58 locus was identified that reduced powdery mildew severity and explained 21% of field variation (P value < 0.01). KASP™ assays were developed from closely linked GBS-SNP markers, a refined genetic map was developed, and four markers that cosegregate with Pm58 were identified. This novel source of powdery mildew-resistance and closely linked genetic markers will support efforts to develop wheat varieties with powdery mildew resistance.

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Acknowledgements

We are grateful to Ryan Parks for planning and conducting detached-leaf assays, with technical assistance from Tyler Cole, Matt Hargrove, and Will Ostrom. We also want to express thanks to Lee Siler for managing field plots.

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Authors and Affiliations

  1. Department of Plant, Soil and Microbial Sciences, Michigan State University, 1066 Bogue Street, Room A286, East Lansing, MI, 48824, USA

    Andrew T. Wiersma, Linda K. Brown & Eric L. Olson

  2. Department of Plant Biology and Center for Genomics-Enabled Plant Science, Michigan State University, 612 Wilson Rd, Room 166, East Lansing, MI, 48824, USA

    Jane A. Pulman

  3. Department of Plant Pathology, North Carolina State University, USDA-ARS Plant Science Research, 2510 Thomas Hall, Campus Box 7616, Raleigh, NC, 27695, USA

    Christina Cowger

Authors
  1. Andrew T. Wiersma
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  2. Jane A. Pulman
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  3. Linda K. Brown
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  4. Christina Cowger
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  5. Eric L. Olson
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Contributions

A.T.W., C.C., and E.L.O designed research, and wrote and edited the manuscript; A.T.W. performed research; A.T.W., L.K.B., C.C., and E.L.O analyzed data; J.A.P. wrote a custom perl program for BLAST parsing.

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Correspondence to Eric L. Olson.

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Communicated by Aimin Zhang.

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Wiersma, A.T., Pulman, J.A., Brown, L.K. et al. Identification of Pm58 from Aegilops tauschii . Theor Appl Genet 130, 1123–1133 (2017). https://doi.org/10.1007/s00122-017-2874-8

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  • DOI: https://doi.org/10.1007/s00122-017-2874-8

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