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Fine genetic mapping of the white immature fruit color gene w to a 33.0-kb region in cucumber (Cucumis sativus L.)

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Abstract

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The white immature fruit color gene w was rapidly mapped to a 33.0-kb region to identify a valuable candidate gene that encodes peroxidase.

Abstract

The skin color of immature fruit is a crucial external trait of cucumbers, and white skin is shared by limited numbers of commercial cultivars. Herein, one BC1 population and two F2 segregating populations were constructed using four inbred parental lines (WD3 × B-2-2 and Q30 × Q24) to investigate the inheritance patterns and chromosomal locations of immature fruit color genes in cucumbers. Consequently, a single recessive gene, w, was identified that controls white immature fruit color. A total of 526 markers, which were derived from published genetic maps, two reference cucumber genomes (“9930” and GY14), and two parents (Q30 and Q24) for which whole-genome sequence information is available, were used to map the target gene w to a 33.0-kb region flanked by two SNP-based markers, ASPCR39262 and ASPCR39229, which are physically located at 39262450 and 39229482 of chromosome 3 (“9930” draft genome assembly), respectively. Gene prediction indicated that four potential genes were located in the target region. One gene that encodes peroxidase is likely to be a valuable candidate gene because quantitative real-time PCR revealed an eightfold difference in its transcriptional level, and several amino acid variations were found when the deduced amino acid sequence was aligned. A co-segregating marker was used synergistically to test its ability to predict the skin colors of 83 dark green/white germplasms, and the validity of its utility in marker-assisted selection was confirmed. Fine mapping of this locus will assist in cloning the gene and in marker-assisted breeding to develop dark green/white cucumber cultivars.

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Acknowledgments

We are grateful for the assistance provided by the laboratory of Yiqun Weng (USDA-ARS, Vegetable Crops Research Unit and Horticultural Department, University of Wisconsin, 1575 Linden Drive, Madison, WI 53706). We also thank Zheng Li for ideas and Dr. Sikandar Hayat and Husain Ahmad for critical reading of the manuscript. This research was supported by a grant from the National Natural Science Foundation of China (Project #31372074).

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  1. College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Hanqiang Liu, Huanwen Meng, Yupeng Pan, Xinjing Liang, Jianqing Jiao, Yuhong Li, Shuxia Chen & Zhihui Cheng

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  1. Hanqiang Liu
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  2. Huanwen Meng
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Correspondence to Zhihui Cheng.

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Liu, H., Meng, H., Pan, Y. et al. Fine genetic mapping of the white immature fruit color gene w to a 33.0-kb region in cucumber (Cucumis sativus L.). Theor Appl Genet 128, 2375–2385 (2015). https://doi.org/10.1007/s00122-015-2592-z

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