Abstract
Key message
Gamma radiation induced a series of structural aberrations involving Thinopyrum bessarabicum chromosome 4J. The aberrations allowed for deletion mapping of 101 4J-specific markers and fine mapping of blue-grained gene BaThb.
Abstract
Irradiation can induce translocations and deletions to assist physically locating genes and markers on chromosomes. In this study, a 12-Gy dosage of 60Co-γ was applied to pollen and eggs of a wheat (Triticum aestivum) landrace Chinese Spring (CS)–Thinopyrum bessarabicum chromosome 4J disomic addition line (DA4J), and the gametes from irradiated plants were fertilized with normal CS eggs or pollen to produce M1 seeds. Based on genomic in situ hybridization analysis of 261 M1 plants, we identified 74 lines carrying structural aberrations involving chromosome 4J with the higher aberration rate in treated pollen (31.2 %) than in the treated eggs (21.3 %). We further identified 43 (53.8 %) lines with structural aberrations on chromosome 4J by analyzing another 80 M1 plants with 74 4J-specific markers, indicating that combining molecular and cytological methods was more efficient for detecting chromosome aberrations. Marker analysis thus was performed prior to cytogenetic identification on M2–M4 seeds to detect chromosome structural aberrations. Sixty-eight M3 lines with structural aberrations on chromosome 4J and six previously obtained chromosome 4J alien lines were then analyzed using 101 chromosome 4J-specific markers. After combining marker results with chromosome aberrations in each line, chromosome 4J was physically divided into 24 segmental blocks with 7 in the short arm and 17 in the long arm. The blue-grained gene BaThb was further mapped into the region corresponding to block 4JL-11. The chromosome aberrations and the physical map developed in this research provide useful stocks and tools for introgression of genes on chromosome 4J into wheat.
Similar content being viewed by others
References
Ashraf M, Foolad MR (2013) Crop breeding for salt tolerance in the era of molecular markers and marker-assisted selection. Plant Breed 132:10–20
Bie TD, Cao YP, Chen PD (2007) Mass production of intergeneric chromosomal translocations through pollen irradiation of Triticum durum–Haynaldia villosa amphiploid. J Integr Plant Biol 49:1619–1626
Chen PD, Zhou B, Qi LL, Liu DJ (1995) Identification of wheat–Haynaldia villosa amphiploid, addition, substitution and translocation lines by in situ hybridization using biotin-labelled genomic DNA as a probe. J Genet Genomics 22:380–386
Chen S, Chen P, Wang X (2008) Inducement of chromosome translocation with small alien segments by irradiating mature female gametes of the whole arm translocation line. Sci China C Life Sci 51:346–352
Chen PD, You CF, Hu Y, Chen SW, Zhou B, Cao AZ, Wang XE (2013) Radiation-induced translocations with reduced Haynaldia villosa chromatin at the Pm21 locus for powdery mildew resistance in wheat. Mol Breed 31:477–484
Cuadrado A, Cardoso M, Jouve N (2008) Physical organization of simple sequence repeats (SSRs) in Triticeae: structural, functional and evolutionary implications. Cytogenet Genome Res 120:210–219
Endo TR (2007) The gametocidal chromosome as a tool for chromosome manipulation in wheat. Chromosome Res 15:67–75
Fukui KN, Suzuki G, Lagudah ES, Rahman S, Appels R, Yamamoto M, Mukai Y (2001) Physical arrangement of retrotransposon-related repeats in centromeric regions of wheat. Plant Cell Physiol 42:189–196
Gao WX, Chen ZJ, Yu JZ, Raska D, Kohel R, Womack J, Stelly D (2004) Wide-cross whole-genome radiation hybrid mapping of cotton (Gossypium hirsutum L.). Genetics 167:1317–1329
Hossain KG, Riera-Lizarazu O, Kalavacharla V, Vales MI, Maan SS, Kianian SF (2004) Radiation hybrid mapping of the species cytoplasm-specific (scs ae) gene in wheat. Genetics 168:415–423
Jiang JM, Friebe B, Gill BS (1994) Recent advances in alien gene transfer in wheat. Euphytica 73:199–212
Kalavacharla V, Hossain K, Gu Y, Riera-Lizarazu O, Vales MI, Bhamidimarri S, Gonzalez-Hernandez JL, Maan SS, Kianian SF (2006) High-resolution radiation hybrid map of wheat chromosome 1D. Genetics 173:1089–1099
Kazi AG, Awais R, Mujeeb-Kazi A (2013) Biotic stress and crop improvement: a wheat focus around novel strategies. In: Hakeem KR, Ahmad P, Ozturk M (eds) Crop improvement: new approaches and modern techniques. Springer, Berlin, pp 239–267. doi:10.1007/978-1-4614-7028-1
King IP, Forster BP, Law CC, Cant KA, Orford SE, Gorham J, Reader S, Miller TE (1997) Introgression of salt-tolerance genes from Thinopyrum bessarabicum into wheat. New Phytol 137:75–81
Liu WX, Danilova TV, Rouse MN, Bowden RL, Friebe B, Gill BS, Pumphrey MO (2013) Development and characterization of a compensating wheat–Thinopyrum intermedium Robertsonian translocation with Sr44 resistance to stem rust (Ug99). Theor Appl Genet 126:1167–1177
Lyderson FV, Carlos RC (2002) Analysis of gamma radiation-induced chromosome variations in maize (Zea mays L). Caryologia 54:319–327
McCarthy LC (1996) Whole genome radiation hybrid mapping. Trends Genet 12:491–493
Michalak de Jimenez MK, Bassi FM, Ghavami F, Simons K, Dizon R, Seetan RI, Alnemer LM, Denton AM, Doğramacı M, Šimková H, Doležel J, Seth K, Luo MC, Dvorak J, Gu YQ, Kianian SF (2013) A radiation hybrid map of chromosome 1D reveals synteny conservation at a wheat speciation locus. Funct Integr Genomics 13:19–32. doi:10.1007/s10142-013-0318-3
Mukai Y, Nakahara Y, Yamamoto M (1993) Simultaneous discrimination of the three genomes in hexaploid wheat by multicolor fluorescence in situ hybridization using total genomic and highly repeated DNA probes. Genome 36:489–494
Niu ZX, Klindworth DL, Friesen TL, Chao S, Jin Y, Cai XW, Xu SS (2011) Targeted introgression of a wheat stem rust resistance gene by DNA marker-assisted chromosome engineering. Genetics 187:1011–1021
Qi Z, Du P, Qian B, Zhuang L, Chen H, Chen T, Shen J, Guo J, Feng Y, Pei Z (2010) Characterization of a wheat–Thinopyrum bessarabicum (T2JS-2BS·2BL) translocation line. Theor Appl Genet 121:589–597
Riera-Lizarazu O, Vales M, Ananiev E, Rines H, Phillips R (2000) Production and characterization of maize chromosome 9 radiation hybrids derived from an oat–maize addition line. Genetics 156:327–339
Sanamyan MF (2003) Evaluation of the effect of pollen irradiation on karyotype variability in M1 cotton plants. Russ J Genet 39:791–798
Shen YF (2011) Development of structural variations of chromosome 4J and physical mapping of the blue-grained gene of Th. bessarabicum. Master’s thesis, Nanjing Agricultural University
Shen YF, Shen J, Dawadondup Zhuang LF, Wang YZ, Pu J, Feng YG, Chu CG, Wang XE, Qi ZJ (2013) Physical localization of a novel blue-grained gene derived from Thinopyrum bessarabicum. Mol Breed 31:195–204
Snape JW, Parker BB, Simpson E, Ainsworth CC, Payne PI, Law CN (1983) The use of irradiated pollen for differential gene transfer in wheat (Triticum aestivum). Theor Appl Genet 65:103–111
Tiwari VK, Riera-Lizarazu O, Gunn HL, Lopez K, Iqbal MJ, Kianian SF, Leonard JM (2012) Endosperm tolerance of paternal aneuploidy allows radiation hybrid mapping of the wheat D-genome and a measure of γ ray-induced chromosome breaks. PLoS One 7:e48815. doi:10.1371/journal.pone.0048
Wang YZ (2013) Development and characterization of small segmental translocations of Thinopyrum bessarabicum and cytological mapping of interest genes. Master’s thesis, Nanjing Agricultural University
Wardrop J, Snape J, Powell W, Machray CG (2002) Constructing plant radiation hybrid panels. Plant J 31:223–228
Wardrop J, Fuller J, Powell W, Machray GC (2004) Exploiting plant somatic radiation hybrids for physical mapping of expressed sequence tags. Theor Appl Genet 108:343–348
Xu SS, Jin Y, Klindworth DL, Wang RC, Cai XW (2009) Evaluation and characterization of seedling resistances to stem rust Ug99 races in wheat–alien species derivatives. Crop Sci 49:2167–2175
Zhang P, Li WL, Fellers J, Friebe B, Gill BS (2004) BAC-FISH in wheat identifies chromosome landmarks consisting of different types of transposable elements. Chromosoma 112:288–299
Zhao RH, Wang HY, Xiao J, Bie TD, Cheng SH, Jia Q, Yuan CX, Zhang RQ, Cao AZ, Chen PD, Wang XE (2013) Induction of 4VS chromosome recombinants using the CS ph1b mutant and mapping of the wheat yellow mosaic virus resistance gene from Haynaldia villosa. Theor Appl Genet 126:2921–2930
Acknowledgments
The authors thank Dr. Mujeeb-Kazi from CIMMYT, Mexico for providing seeds of the CS–Th. bessarabicum amphiploid, and Dr. Gill from Plant Pathology Department, Kansas State University, Manhattan, KS, USA, for providing clones of pSc119.2, pAs1 and 676D4. This project was supported by the National Natural Science Foundation of China (31170302), the 111 Project (B08025) and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
Conflict of interest
The authors declare no conflict of interest.
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by S. S. Xu.
J. Pu, Q. Wang and Y. Shen have contributed equally to this research.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Pu, J., Wang, Q., Shen, Y. et al. Physical mapping of chromosome 4J of Thinopyrum bessarabicum using gamma radiation-induced aberrations. Theor Appl Genet 128, 1319–1328 (2015). https://doi.org/10.1007/s00122-015-2508-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00122-015-2508-y