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Relationships between wheat grain physical characteristics studied through near-isogenic lines with distinct puroindoline-b allele

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Abstract

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Genetic (different forms of puroindoline-b) and environment (through variations in vitreousness), have important effects on wheat grain mechanical properties. The two methods of hardness measurements (NIRS, SKCS) do not give the same information.

Abstract

Bread wheat near-isogenic lines differing in hardness, due to distinct puroindoline-b alleles (the wild type, Pinb-D1a, or the mutated forms, Pinb-D1b or Pinb-D1d), were grown for three years in seven sites and under two nitrogen fertilization levels, to study genetic and environmental effects on grain mechanical properties. Two methods, Near-Infrared Reflectance Spectroscopy (NIRS) and Single Kernel Characterization System (SKCS), currently used for grain hardness characterization, were carried out. Grain vitreousness, which is known to affect the grain mechanical behavior but is generally not studied, was also measured, as well as three other characters (Thousand Grain Weight, Test Weight and protein content). The relationships between the different characters were studied. Results revealed a clear effect of the different Pinb-D1 alleles on NIRS hardness, and a marked impact of the environmental conditions on vitreousness. SKCS hardness was influenced by both Pinb-D1 alleles and environmental conditions. The relationship between SKCS and NIRS hardness was strong when considering together soft and hard genotypes, but moderate within a class of genetical hardness. Vitreousness had only a weak effect on NIRS hardness, whereas vitreousness and SKCS values were strongly correlated, with two distinct regressions for soft and hard genotypes. Vitreousness was positively related to protein content, especially in the case of hard genotypes, which were able to reach high vitreousness values never observed for soft genotypes.

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Acknowledgments

We are grateful to all the INRA and UFS agents implicated in the field trials or the measurements of grain parameters, and to S. Alquié and N. Beaubras (INRA non permanent staff) for their measurement of grain vitreousness.

This work was granted by FSOV (Fonds de Soutien à l’Obtention Végétale, 2007-2010).

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standards

The authors declare that the experiments described in this manuscript comply with the current laws in France.

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Authors and Affiliations

  1. INRA, UMR 1095 Génétique Diversité et Ecophysiologie des Céréales, 234 avenue du Brézet, 63100, Clermont-Ferrand, France

    François-Xavier Oury

  2. INRA, UMR 1208, Ingénierie des Agropolymères et Technologies Emergentes, 2 Place Viala, 34060, Montpellier Cedex 01, France

    P. Lasme, J. Abecassis & V. Lullien-Pellerin

  3. UFS, 17 rue du Louvre, 75001, Paris, France

    C. Michelet

  4. INRA-UPS-AgroParisTech, UMR de Génétique Végétale, Ferme du Moulon, 91190, Gif-Sur-Yvette, France

    M. Rousset

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  1. François-Xavier Oury
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  2. P. Lasme
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  3. C. Michelet
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  4. M. Rousset
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  5. J. Abecassis
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  6. V. Lullien-Pellerin
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Correspondence to François-Xavier Oury.

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Communicated by I. D. Godwin.

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Oury, FX., Lasme, P., Michelet, C. et al. Relationships between wheat grain physical characteristics studied through near-isogenic lines with distinct puroindoline-b allele. Theor Appl Genet 128, 913–929 (2015). https://doi.org/10.1007/s00122-015-2479-z

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  • DOI: https://doi.org/10.1007/s00122-015-2479-z

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