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Fine mapping and characterization of Sr21, a temperature-sensitive diploid wheat resistance gene effective against the Puccinia graminis f. sp. tritici Ug99 race group

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Abstract

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The diploid wheat stem rust resistance gene Sr21 confers temperature-sensitive resistance to isolates of the Ug99 group and maps to the middle of the long arm of chromosome 2A m.

Abstract

A race of Puccinia graminis f. sp. tritici, the causal pathogen of stem rust of wheat, known as Ug99, and its variants, are virulent to plants carrying stem rust resistance genes currently deployed in most wheat cultivars worldwide. Therefore, identification, mapping and deployment of effective resistance genes are critical to reduce this threat. Resistance gene Sr21 identified in diploid wheat T. monococcum can be effective against races from the Ug99 race group, but both susceptible and partial resistant reactions have been reported in previous studies. To clarify this conflicting information we screened four monogenic lines with Sr21 and four susceptible controls with 16 Pgt isolates including five isolates of the Ug99 race group under three different temperatures and three different photoperiods. We observed that, temperature influences the interaction between monogenic lines with Sr21 and Ug99 race group isolates, and may be one source of previous inconsistencies. This result indicates that, although Sr21 confers partial resistance against Ug99, its effectiveness can be modulated by environmental conditions and should not be deployed alone. Using two large diploid wheat-mapping populations (total 3,788 F2 plants) we mapped Sr21 approximately 50 cM from the centromere on the long arm of chromosome 2Am within a 0.20 cM interval flanked by sequence-based markers FD527726 and EX594406. The closely linked markers identified in this study will be useful to reduce the T. monococcum segments introgressed into common wheat, accelerate Sr21 deployment in wheat breeding programs, and facilitate the map-based cloning of this gene.

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Acknowledgments

This project was supported by the Borlaug Global Rust Initiative, by the National Research Initiative Competitive Grants 2011-68002-30029 (Triticeae-CAP) and 2012-67013-19401 from the United States Department of Agriculture (USDA) National Institute of Food and Agriculture (NIFA), USDA-Agricultural Research Service appropriated project 3640-21220-021-00, USDA National Plant Disease Recovery System, and by support from the Howard Hughes Medical Institute and the Gordon and Betty Moore Foundation. We thank Mariana Padilla for excellent technical support.

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The authors declare that they have no conflict of interest.

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Correspondence to Jorge Dubcovsky.

Additional information

Communicated by Evans Lagudah.

S. Chen, M. N. Rouse and W. Zhang contributed equally to this work.

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122_2015_2460_MOESM1_ESM.pdf

Supplementary material 1 (PDF 105 kb) Supplementary Table S1 Seedling infection types of wheat lines with and without Sr21 to diverse isolates (and corresponding races) of Puccinia graminis f. sp. tritici at various temperatures and photoperiods

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Chen, S., Rouse, M.N., Zhang, W. et al. Fine mapping and characterization of Sr21, a temperature-sensitive diploid wheat resistance gene effective against the Puccinia graminis f. sp. tritici Ug99 race group. Theor Appl Genet 128, 645–656 (2015). https://doi.org/10.1007/s00122-015-2460-x

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  • DOI: https://doi.org/10.1007/s00122-015-2460-x

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