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QTL for resistance to root lesion nematode (Pratylenchus thornei) from a synthetic hexaploid wheat source

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A whole genome average interval mapping approach identified eight QTL associated with P. thornei resistance in a DH population from a cross between the synthetic-derived wheat Sokoll and cultivar Krichauff.

Abstract

Pratylenchus thornei are migratory nematodes that feed and reproduce within the wheat root cortex, causing cell death (lesions) resulting in severe yield reductions globally. Genotypic selection using molecular markers closely linked to Pratylenchus resistance genes will accelerate the development of new resistant cultivars by reducing the need for laborious and expensive resistance phenotyping. A doubled haploid wheat population (150 lines) from a cross between the synthetic-derived cultivar Sokoll (P. thornei resistant) and cultivar Krichauff (P. thornei moderately susceptible) was used to identify quantitative trait loci (QTL) associated with P. thornei resistance. The resistance identified in the glasshouse was validated in a field trial. A genetic map was constructed using Diversity Array Technology and the QTL regions identified were further targeted with simple sequence repeat (SSR) and single-nucleotide polymorphism (SNP) markers. Six significant and two suggestive P. thornei resistance QTL were detected using a whole genome average interval mapping approach. Three QTL were identified on chromosome 2B, two on chromosome 6D, and a single QTL on each of chromosomes 2A, 2D and 5D. The QTL on chromosomes 2BS and 6DS mapped to locations previously identified to be associated with Pratylenchus resistance. Together, the QTL on 2B (QRlnt.sk-2B.1–2B.3) and 6D (QRlnt.sk-6D.1 and 6D.2) explained 30 and 48 % of the genotypic variation, respectively. Flanking PCR-based markers based on SSRs and SNPs were developed for the major QTL on 2B and 6D and provide a cost-effective high-throughput tool for marker-assisted breeding of wheat with improved P. thornei resistance.

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Acknowledgments

This research was supported by the Grains Research and Development Corporation (GRDC) through a Grains Industry Research Scholarship to K.J. Linsell and for funding the original screening of the mapping population and field evaluations. The project was also supported by the South Australian Research and Development Institute (SARDI) and Molecular Plant Breeding Cooperative Research Centre (MPBCRC). The authors would like to thank K. Willsmore for MapManger QTX training, to the SARDI Nematology Group, especially D. Pounsett for assistance with culturing of the nematodes, to A. McKay (SARDI) and A. Cook (Minnipa Agricultural Centre) for coordinating the field trials, and to I. Riley at SARDI and K. Davies at the University of Adelaide for manuscript editing. Further thanks are to H. Eagles, University of Adelaide, for the information on pedigrees and COP value.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. South Australian Research and Development Institute, University of Adelaide, Molecular Plant Breeding CRC, GPO Box 397, Adelaide, SA, 5001, Australia

    Katherine J. Linsell, Muhammad S. Rahman, Rowena S. Davey, Hugh Wallwork & Klaus H. Oldach

  2. Australian Centre for Plant Functional Genomics, Glen Osmond, SA, 5064, Australia

    Muhammad S. Rahman

  3. Department of Environment and Primary Industry, AgriBiosciences Centre Bundoora, Bundoora, VIC, 3083, Australia

    Kerrie L. Forrest & Matthew J. Hayden

  4. School of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, SA, 5064, Australia

    Julian D. Taylor & Beverley J. Gogel

  5. CRC for National Plant Biosecurity, University Drive, University of Canberra, Bruce, ACT, 2617, Australia

    Sharyn P. Taylor

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  1. Katherine J. Linsell
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  2. Muhammad S. Rahman
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  3. Julian D. Taylor
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  5. Beverley J. Gogel
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  6. Hugh Wallwork
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  7. Kerrie L. Forrest
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  10. Klaus H. Oldach
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Corresponding author

Correspondence to Klaus H. Oldach.

Additional information

Communicated by M. Xu.

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Supplementary Fig. 1

Whole genome average interval mapping iterations for the Sokoll x Krichauff doubled haploid wheat population. Supplementary material 1 (DOCX 232 kb)

Supplementary Table 1

SNP sequences on iSelect 90 K SNP wheat bead chip linked to Pratylenchus thornei resistance QTL on chromosome 2B and 6D in the Sokoll x Krichauff wheat. Supplementary material 2 (DOCX 15 kb)

Supplementary Table 2

STS markers derived from DArT sequences and mapped in the Sokoll x Krichauff population to chromosomes 2B and 6D. Supplementary material 3 (DOCX 12 kb)

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Linsell, K.J., Rahman, M.S., Taylor, J.D. et al. QTL for resistance to root lesion nematode (Pratylenchus thornei) from a synthetic hexaploid wheat source. Theor Appl Genet 127, 1409–1421 (2014). https://doi.org/10.1007/s00122-014-2308-9

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