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Allelic variation in genes contributing to glycoalkaloid biosynthesis in a diploid interspecific population of potato

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Variation for allelic state within genes of both primary and secondary metabolism influences the quantity and quality of steroidal glycoalkaloids produced in potato leaves.

Abstract

Genetic factors associated with the biosynthesis and accumulation of steroidal glycoalkaloids (SGAs) in potato were addressed by a candidate gene approach and whole genome single nucleotide polymorphism (SNP) genotyping. Allelic sequences spanning coding regions of four candidate genes [3-hydroxy-3-methylglutaryl coenzyme A reductase 2 (HMG2); 2,3-squalene epoxidase; solanidine galactosyltransferase; and solanidine glucosyltransferase (SGT2)] were obtained from two potato species differing in SGA composition: Solanum chacoense (chc 80-1) and Solanum tuberosum group Phureja (phu DH). An F2 population was genotyped and foliar SGAs quantified. The concentrations of α-solanine, α-chaconine, leptine I, leptine II and total SGAs varied broadly among F2 individuals. F2 plants with chc 80-1 alleles for HMG2 or SGT2 accumulated significantly greater leptines and total SGAs compared to plants with phu DH alleles. Plants with chc 80-1 alleles at both loci expressed the greatest levels of total SGAs, α-solanine and α-chaconine. A significant positive correlation was found between α-solanine and α-chaconine accumulation as well as between leptine I and leptine II. A whole genome SNP genotyping analysis of an F2 subsample verified the importance of chc 80-1 alleles at HMG2 and SGT2 for SGA synthesis and accumulation and suggested additional candidate genes including some previously associated with SGA production. Loci on five and seven potato pseudochromosomes were associated with synthesis and accumulation of SGAs, respectively. Two loci, on pseudochromosomes 1 and 6, explained phenotypic segregation of α-solanine and α-chaconine synthesis. Knowledge of the genetic factors influencing SGA production in potato may assist breeding for pest resistance.

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Acknowledgments

This research was supported by Research Grant No. IS-4134-08 R from BARD, The United States—Israel Binational Agricultural Research and Development Fund. We thank Suzanne Piovano for technical assistance, Maichel Miguel Aguayo Bustos for programming help, Anne Ryan of the Laboratory for Interdisciplinary Statistical Analysis LISA for statistical consultation.

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Authors and Affiliations

  1. Department of Horticulture, Virginia Polytechnic Institute and State University, Blacksburg, VA, 24061, USA

    Norma Constanza Manrique-Carpintero, James G. Tokuhisa & Richard E. Veilleux

  2. Department of Vegetable Research, The Volcani Center, Agricultural Research Organization, Bet Dagan, Israel

    Idit Ginzberg

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  1. Norma Constanza Manrique-Carpintero
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  2. James G. Tokuhisa
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  3. Idit Ginzberg
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  4. Richard E. Veilleux
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Correspondence to Richard E. Veilleux.

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Communicated by G. Bryan.

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Manrique-Carpintero, N.C., Tokuhisa, J.G., Ginzberg, I. et al. Allelic variation in genes contributing to glycoalkaloid biosynthesis in a diploid interspecific population of potato. Theor Appl Genet 127, 391–405 (2014). https://doi.org/10.1007/s00122-013-2226-2

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