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Co-segregation analysis and mapping of the anthracnose Co-10 and angular leaf spot Phg-ON disease-resistance genes in the common bean cultivar Ouro Negro

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Abstract

Anthracnose (ANT) and angular leaf spot (ALS) are devastating diseases of common bean (Phaseolus vulgaris L.). Ouro Negro is a highly productive common bean cultivar, which contains the Co-10 and Phg-ON genes for resistance to ANT and ALS, respectively. In this study, we performed a genetic co-segregation analysis of resistance to ANT and ALS using an F2 population from the Rudá × Ouro Negro cross and the F2:3 families from the AND 277 × Ouro Negro cross. Ouro Negro is resistant to races 7 and 73 of the ANT and race 63-39 of the ALS pathogens. Conversely, cultivars AND 277 and Rudá are susceptible to races 7 and 73 of ANT, respectively. Both cultivars are susceptible to race 63-39 of ALS. Co-segregation analysis revealed that Co-10 and Phg-ON were inherited together, conferring resistance to races 7 and 73 of ANT and race 63-39 of ALS. The Co-10 and Phg-ON genes were co-segregated and were tightly linked at a distance of 0.0 cM on chromosome Pv04. The molecular marker g2303 was linked to Co-10 and Phg-ON at a distance of 0.0 cM. Because of their physical linkage in a cis configuration, the Co-10 and Phg-ON resistance alleles are inherited together and can be monitored with great efficiency using g2303. The close linkage between the Co-10 and Phg-ON genes and prior evidence are consistent with the existence of a resistance gene cluster at one end of chromosome Pv04, which also contains the Co-3 locus and ANT resistance quantitative trait loci. These results will be very useful for breeding programs aimed at developing bean cultivars with ANT and ALS resistance using marker-assisted selection.

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Acknowledgments

This research was financially supported by the National Council for Scientific and Technological Development (CNPq). Anelise S. Cruz was supported by a scholarship from Capes. We would like to thank Andressa Gonçalves Vidigal for additional review of the manuscript. M.C. Gonçalves-Vidigal and P.S. Vidigal Filho are grateful for grants from CNPq.

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Authors and Affiliations

  1. Departamento de Agronomia, Universidade Estadual de Maringá, Av. Colombo, 5790, Maringá, Paraná, 87020-900, Brazil

    M. C. Gonçalves-Vidigal, A. S. Cruz, G. F. Lacanallo, P. S. Vidigal Filho, L. L. Sousa & C. M. N. A. Pacheco

  2. Genomics and Bioinformatics Program, Department of Plant Sciences, North Dakota State University, Fargo, ND, 58105, USA

    P. McClean

  3. Section of Crop and Ecosystem Sciences, Department of Plant Sciences/MS1, University of California, Davis, CA, 95616-8780, USA

    P. Gepts

  4. Soybean Genomics and Improvement Laboratory, USDA-ARS, BARC-West, Beltsville, MD, 20705, USA

    M. A. Pastor-Corrales

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  1. M. C. Gonçalves-Vidigal
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  2. A. S. Cruz
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  3. G. F. Lacanallo
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  4. P. S. Vidigal Filho
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  5. L. L. Sousa
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  7. P. McClean
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  8. P. Gepts
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  9. M. A. Pastor-Corrales
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Correspondence to M. A. Pastor-Corrales.

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Gonçalves-Vidigal, M.C., Cruz, A.S., Lacanallo, G.F. et al. Co-segregation analysis and mapping of the anthracnose Co-10 and angular leaf spot Phg-ON disease-resistance genes in the common bean cultivar Ouro Negro. Theor Appl Genet 126, 2245–2255 (2013). https://doi.org/10.1007/s00122-013-2131-8

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