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Targeted discovery of quantitative trait loci for resistance to northern leaf blight and other diseases of maize

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Abstract

To capture diverse alleles at a set of loci associated with disease resistance in maize, heterogeneous inbred family (HIF) analysis was applied for targeted QTL mapping and near-isogenic line (NIL) development. Tropical maize lines CML52 and DK888 were chosen as donors of alleles based on their known resistance to multiple diseases. Chromosomal regions (“bins”; n = 39) associated with multiple disease resistance (MDR) were targeted based on a consensus map of disease QTLs in maize. We generated HIFs segregating for the targeted loci but isogenic at ~97% of the genome. To test the hypothesis that CML52 and DK888 alleles at MDR hotspots condition broad-spectrum resistance, HIFs and derived NILs were tested for resistance to northern leaf blight (NLB), southern leaf blight (SLB), gray leaf spot (GLS), anthracnose leaf blight (ALB), anthracnose stalk rot (ASR), common rust, common smut, and Stewart’s wilt. Four NLB QTLs, two ASR QTLs, and one Stewart’s wilt QTL were identified. In parallel, a population of 196 recombinant inbred lines (RILs) derived from B73 × CML52 was evaluated for resistance to NLB, GLS, SLB, and ASR. The QTLs mapped (four for NLB, five for SLB, two for GLS, and two for ASR) mostly corresponded to those found using the NILs. Combining HIF- and RIL-based analyses, we discovered two disease QTLs at which CML52 alleles were favorable for more than one disease. A QTL in bin 1.06–1.07 conferred resistance to NLB and Stewart’s wilt, and a QTL in 6.05 conferred resistance to NLB and ASR.

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Acknowledgments

We thank Randall Wisser for integrating the markers used in the HIF analysis onto the disease QTL consensus map, Erik Stromberg for his help with field trials in Virginia, Kent Loeffler for photography, and Pioneer Hi-Bred International Inc. for the supply of sorghum grains used in production of inoculum. Seed for the F5 families and the RIL population from B73 × CML52 were generously provided by Edward Buckler. Seed for the F6 families from S11 × DK888 were kindly provided by Major Goodman. We appreciate help from Oliver Ott, Kristen Kennedy, Zura Kerdieh, and Donna Stephens regarding research aspects. The work was funded by The CGIAR Generation Challenge Program, The McKnight Foundation, USDA-ARS, The Bill & Melinda Gates Foundation, and Ministry of Education, Taiwan.

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  1. Chia-Lin Chung

    Present address: Department of Plant Pathology and Microbiology, National Taiwan University, Taipei, 10617, Taiwan

  2. Jesse Poland

    Present address: U.S. Department of Agriculture, Agricultural Research Service, Department of Agronomy, Kansas State University, Manhattan, KS, 66506, USA

  3. Ellie Walsh

    Present address: Department of Plant Pathology, The Ohio State University, Wooster, OH, 44691, USA

Authors and Affiliations

  1. Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY, 14853, USA

    Chia-Lin Chung, Joy Longfellow, Ellie Walsh & Rebecca Nelson

  2. Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY, 14853, USA

    Jesse Poland, Jacqueline Benson & Rebecca Nelson

  3. Department of Crop Science, North Carolina State University, Raleigh, NC, 27695, USA

    Kristen Kump

  4. U.S. Department of Agriculture, Agricultural Research Service, Department of Plant Pathology, North Carolina State University, Raleigh, NC, 27695, USA

    Peter Balint-Kurti

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Correspondence to Rebecca Nelson.

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Chung, CL., Poland, J., Kump, K. et al. Targeted discovery of quantitative trait loci for resistance to northern leaf blight and other diseases of maize. Theor Appl Genet 123, 307–326 (2011). https://doi.org/10.1007/s00122-011-1585-9

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