Theoretical and Applied Genetics

, Volume 122, Issue 3, pp 543–553

Genotypic effects on the frequency of homoeologous and homologous recombination in Brassica napus × B. carinata hybrids

  • Annaliese S. Mason
  • Matthew N. Nelson
  • Marie-Claire Castello
  • Guijun Yan
  • Wallace A. Cowling
Original Paper

DOI: 10.1007/s00122-010-1468-5

Cite this article as:
Mason, A.S., Nelson, M.N., Castello, MC. et al. Theor Appl Genet (2011) 122: 543. doi:10.1007/s00122-010-1468-5

Abstract

We investigated the influence of genotype on homoeologous and homologous recombination frequency in eight different Brassica napus (AACnCn) × B. carinata (BBCcCc) interspecific hybrids (genome composition CnCcAB). Meiotic recombination events were assessed through microsatellite marker analysis of 67 unreduced microspore-derived progeny. Thirty-four microsatellite markers amplified 83 A-, B-, Cn- and Cc-genome alleles at 64 loci, of which a subset of seven markers amplifying 26 alleles could be used to determine allele copy number. Hybrid genotypes varied significantly in loss of A- and B-genome alleles (P < 0.0001), which ranged from 6 to 22% between hybrid progeny sets. Allele copy number analysis revealed 19 A–C, 3 A–B and 10 B–C duplication/deletion events attributed to homoeologous recombination. Additionally, 55 deletions and 19 duplications without an accompanying dosage change in homoeologous alleles were detected. Hybrid progeny sets varied in observed frequencies of loss, gain and exchange of alleles across the A and B genomes as well as in the diploid C genome. Self-fertility in hybrid progeny decreased as the loss of B-genome loci (but not A-genome loci) increased. Hybrid genotypes with high levels of homologous and homoeologous exchange may be exploited for genetic introgressions between B. carinata and B. napus (canola), and those with low levels may be used to develop stable synthetic Brassica allopolyploids.

Supplementary material

122_2010_1468_MOESM1_ESM.pdf (53 kb)
Supplementary material 1 (PDF 52 kb)
122_2010_1468_MOESM2_ESM.pdf (46 kb)
Supplementary material 2 (PDF 45 kb)

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Annaliese S. Mason
    • 1
  • Matthew N. Nelson
    • 1
  • Marie-Claire Castello
    • 1
  • Guijun Yan
    • 1
  • Wallace A. Cowling
    • 1
  1. 1.School of Plant Biology and The UWA Institute of Agriculture, Faculty of Natural and Agricultural SciencesThe University of Western AustraliaCrawleyAustralia